Validated spectrophotometric methods for the determination of bifonazole in pharmaceuticals by charge transfer complexation

Abstract

Two simple and selective visible spectrophotometric methods were developed for assay of bifonazole in pure drug and in its pharmaceutical formulation. The developed methods were based on the charge transfer complexation reaction of bifonazole with 2,5-dichloro-3,6-dihydroxy-1,4-benzoquinone (CAA) for method A and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) for method B, resulting in the formation of colored complex. The colored reaction products were quantitated spectrophotometrically at 517 nm and 457 nm for bifonazole-CAA and bifonazole-DDQ complexes, respectively. The complexes obeyed Beer's law in the concentration range of 50.00-400.00 and 5.00-50.00 lg mL À1 with molar absorptivities at 0.0956 · 10 4 and 0.6953 · 10 4 L mol À1 cm À1 for method A and method B, respectively. The proposed procedures were successfully applied for the quantitative determination of bifonazole in its pharmaceutical formulation. The solid charge transfer complexes of bifonazole with each of the p-acceptor reagent were also synthesized and characterized by FT-IR spectroscopy.ª 2014 Production and hosting by Elsevier B.V. on behalf of University of Bahrain.

Spectrophotometric method for pregabalin determination: An experimental design approach for method development

“…The reagents include potassium iodate and potassium iodide (Gujral et al, 2009), fluorescamine, 2,4-diclorofluorobenzene and 2,3,5,6-tetrachloro-1, 4-benzoquinone (Shaalan, 2010), ninhydrin (Bali and Gaur, 2011), 1,2-naphthoquinone-4-sulfonate and 2,4-dinitrofluoro benzene (Walash et al, 2011), ascorbic acid and salicylaldehyde (Hammad and Abdallah, 2012) and bromocresol green (Merin et al, 2013). In the current literature Ismail et al (2014) and Kumble and Narayana (2014) also developed spectrophotometric methods for the determination of bifonazole and febantel, respectively. In methods mentioned in the foregoing, the experimental variables, such as the reagent concentration, reagent volume, heating temperature, heating time and pH of the reaction mixture were optimized using the one variable at a time method (OVAT).…”