Validating biochemical features at the genome level in the Antarctic bacterium <i>Hymenobacter</i> sp. strain UV11

Marizcurrena, Juan José; Herrera, Lorena; Costábile, Alicia; Morales, Danilo; Villadóniga, Carolina; Eizmendi, Agustina; Davyt, Danilo; Castro‐Sowinski, Susana

doi:10.1093/femsle/fnz177

Cited by 16 publications

(7 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The genus Hymenobacter accommodates species that are aerobic and non-motile. The species grow under conditions of extremely low organic substances using limited carbon sources like isoprene, sugars, amino-acids, organic-acids and alcohols (22). Flavobacterium spp.…”

Section: Bacterial Organisms Associated With Annona Muricata and Malu...mentioning

confidence: 99%

Comparative Bacterial Metagenomics of Soursop (Annona muricata L.) and Apple (Malus domestica B.)

2022

View full text Add to dashboard Cite

Illumina Next Generation Sequencing (NGS) platform targeting the conserved regions of bacteria ribosomal DNA (16s rRNA) was used to identify the bacterial community associated with soursop (Annona muricata L.). The aim of this work is to compare the diversities of the bacterial communities of Annona muricata and Malus domestica (obtained from National Centre for Biotechnology Information (NCBI) database). The functional genes in these communities were also predicted. A total of 167,693 high quality reads was obtained from Annona muricata and Malus domestica. Clustering on GREENGENES database revealed 570 Operational Taxonomic Units (OTUs). Alpha-diversity indices indicated high diversity and abundance of microbial community. Taxonomic analysis revealed that bacterial community was grouped into 24 phyla and 455 genera. The microbiome of the samples was dominated by distinct populations of four phyla viz Proteobacteria (58.41%), Bacteroidetes (18.59%), Actinobacteria (11.13%) and Firmicutes (7.29%). The functional genes were predicted for 16S rRNA gene sequences based on Kyoto Encyclopedia of Genes and Genomes (KEGG) which indicated amino acid metabolism, carbohydrate metabolism, xenobiotics biodegradation and lipid metabolism, metabolism of terpenoids and polypeptides and biosynthesis of other secondary metabolites as predominant metabolic categories. Thus, the study revealed the structure of microbial community and functional genes composition in A. muricata and M. domestica fruits and this will help to expand the knowledge concerning the structure of plant-associated bacterial communities, revealing valuable information of their impact and indicating their crucial roles in evolutionary and ecological processes.

show abstract

Section: Bacterial Organisms Associated With Annona Muricata and Malu...mentioning

confidence: 99%

Comparative Bacterial Metagenomics of Soursop (Annona muricata L.) and Apple (Malus domestica B.)

2022

View full text Add to dashboard Cite

show abstract

“…5 ). Synthesis of starch or glycogen can be used to store energy which is important in oligotrophic conditions [ 73 , 74 ]. Carbon starvation protein coding regions were also present in three MAGs and the unbinned metagenomic reads.…”

Section: Resultsmentioning

confidence: 99%

“…Group 1 l [NiFe]-hydrogenase [ 67 ] was found in the unbinned metagenomic reads, as well as three MAGs identified as the families Chitinophagaceae , Pyrinomonadaceae , and Cyclobacteriaceae . The potential for the oxidation of atmospheric hydrogen by Group 1 h and 1 l [NiFe]-hydrogenase has been found in multiple metagenomic studies of Antarctic deserts, often with hydrogen oxidation genes often being present across all metagenomes [ 33 , 74-76 ]. Oxidation of atmospheric H 2 by membrane bound hydrogenases produces energy through the electron transport chain and produces metabolic water which could be a significant source of hydration for cells in extremely arid environments [ 76 ].…”

Section: Resultsmentioning

confidence: 99%

Active microbiota persist in dry permafrost and active layer from Elephant Head, Antarctica

Wood,

Bruinink,

Trembath-Reichert

et al. 2024

ISME Communications

View full text Add to dashboard Cite

Dry permafrost is a challenging environment for microbial life due to the cold, dry, and often oligotrophic conditions. In 2016, Elephant Head, Antarctica was confirmed as the second site on Earth to contain dry permafrost. It is geographically distinct from the McMurdo Dry Valleys where dry permafrost has been studied previously. Here we present the first study of the microbial activity, diversity, and functional potential of Elephant Head dry permafrost. Microbial activity was measured using radiorespiration assays with radiolabelled acetate as a carbon source at 5, 0 and − 5°C. Low, but detectable, rates of microbial activity were measured in some samples at 0 and − 5°C. This is distinct from previous studies of McMurdo Dry Valley dry permafrost which concluded that dry permafrost represents a cold-arid limit to life on the planet. The isolation of cold adapted organisms from these soils, including one capable of sub-zero growth further supports that Elephant Head dry active layer and dry permafrost harbor viable microbial life which may be active in situ. Metagenomic, 16S rRNA gene and ITS and amplicon sequencing identified similar microbial communities to other Antarctic and cold environments. The Elephant Head microbial community appears adapted for survival in cold, dry, and oligotrophic conditions based on the presence of cold adaptation and stress response genes in the metagenomes. Together our results show that dry permafrost environments do not exclude active microbial life at sub-zero temperatures and suggests that the cold, dry soils of Mars may also not be as inhospitable as previously thought.

show abstract

“…Cytophagales are Gram-negative bacteria that are known to be less resistant to UV radiation than Gram-positive bacteria 37 . In addition, 91.68% of Cytophagales sequences belong to Hymenobacter , a heterotrophic genus composed of mostly heterotrophic, aerobic, Gram-negative bacteria which have been described in extreme environments like Antarctica, where a strain with extremely high resistance to solar radiation was isolated 38 , 39 . Armatimonadota and Frankiales were never reported as abundant inhabitants of cryoconite holes in other geographical areas.…”

Section: Discussionmentioning

confidence: 99%

Geographical variability of bacterial communities of cryoconite holes of Andean glaciers

Pittino

Ambrosini

Seeger

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Cryoconite holes, ponds full of melting water with sediment on the bottom, are hotspots of biodiversity on glacier surfaces and host dynamic micro-ecosystems. They have been extensively investigated in different areas of the world (e.g., the Arctic, Antarctic, Alps, and Himalaya), but so far no study has described the bacterial communities of the glaciers in the Andes, the world’s longest mountain range. In this study, we describe the bacterial communities of three small (< 2 km2) high-elevation (< 4200 m a.s.l.) glaciers of the Central Andes (Iver, East Iver and Morado glaciers) and two large (> 85 km2) glaciers of the Patagonian Andes (Exploradores and Perito Moreno glaciers) whose ablation tongues reach low altitude (< 300 m a.s.l.). Results show that the bacterial communities were generally similar to those observed in the cryoconite holes of other continents, but with few cyanobacteria (0.5% of sequences). The most abundant orders were Betaproteobacteriales, Cytophagales, Chitinophagales, Acetobacterales, Frankiales, Armatimonadales, Sphingobacteriales, Rhizobiales, Bacteroidales, Sphingomonadales, and Micrococcales. The bacterial communities differed between glaciers and both water pH and O2 concentration appeared to influence the bacterial community composition. This work thus provides the first description of the bacterial communities in cryoconite holes of South American glaciers.

show abstract

Validating biochemical features at the genome level in the Antarctic bacterium Hymenobacter sp. strain UV11

Cited by 16 publications

References 57 publications

Comparative Bacterial Metagenomics of Soursop (Annona muricata L.) and Apple (Malus domestica B.)

Comparative Bacterial Metagenomics of Soursop (Annona muricata L.) and Apple (Malus domestica B.)

Active microbiota persist in dry permafrost and active layer from Elephant Head, Antarctica

Geographical variability of bacterial communities of cryoconite holes of Andean glaciers

Contact Info

Product

Resources

About