Validating porcine  SCD  haplotype effects on fatty acid desaturation and fat deposition in different genetic backgrounds

Fernández, Ana; Óvilo, Cristina; Barragán, C.; Rodríguez, M. C.; Silió, L.; Folch, Josep María; Fernández, Almudena

doi:10.1016/j.livsci.2017.09.021

Cited by 12 publications

(19 citation statements)

References 34 publications

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“…These SNPs may be used in breeding schemes with the purpose of improving meat quality by favoring a better fatty acid profile in terms of nutritional, organoleptic, technological and manufacturing properties. In the case of SCD mutation, the favorable allele is already at high frequencies in most populations, in agreement with the high desaturation potential and favorable fatty acid profile and meat quality parameters observed in these breeds [ 74 , 94 – 101 ]but there are few as Black Slavonian and Mora Romagnola with selection possibilities. For ACSL4 , in contrast, high level of segregation is observed in most breeds.…”

Section: Resultssupporting

confidence: 69%

“…The SCD is a very relevant enzyme because it catalyzes the desaturation of palmitic and stearic to palmitoleic and oleic acids. For this gene a mutation g.2228T>C (g.111461751C>T in Sscrofa11.1), positioned in the core sequence of several putative transcription factor binding sites, has been repeatedly associated to MUFA content in different genetic backgrounds [ 52 , 94 ]. There are several plausible mechanisms by which allele T enhances 18∶1/18∶0 ratio and, consequently, the proportion of monounsaturated to saturated fatty acids, increasing meat quality.…”

Section: Resultsmentioning

confidence: 99%

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Diversity across major and candidate genes in European local pig breeds

Muñoz¹,

Bozzi

García³

et al. 2018

PLoS ONE

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The aim of this work was to analyse the distribution of causal and candidate mutations associated to relevant productive traits in twenty local European pig breeds. Also, the potential of the SNP panel employed for elucidating the genetic structure and relationships among breeds was evaluated. Most relevant genes and mutations associated with pig morphological, productive, meat quality, reproductive and disease resistance traits were prioritized and analyzed in a maximum of 47 blood samples from each of the breeds (Alentejana, Apulo-Calabrese, Basque, Bísara, Majorcan Black, Black Slavonian (Crna slavonska), Casertana, Cinta Senese, Gascon, Iberian, Krškopolje (Krškopoljski), Lithuanian indigenous wattle, Lithuanian White Old Type, Mora Romagnola, Moravka, Nero Siciliano, Sarda, Schwäbisch-Hällisches Schwein (Swabian Hall pig), Swallow-Bellied Mangalitsa and Turopolje). We successfully analyzed allelic variation in 39 polymorphisms, located in 33 candidate genes. Results provide relevant information regarding genetic diversity and segregation of SNPs associated to production and quality traits. Coat color and morphological trait-genes that show low level of segregation, and fixed SNPs may be useful for traceability. On the other hand, we detected SNPs which may be useful for association studies as well as breeding programs. For instance, we observed predominance of alleles that might be unfavorable for disease resistance and boar taint in most breeds and segregation of many alleles involved in meat quality, fatness and growth traits. Overall, these findings provide a detailed catalogue of segregating candidate SNPs in 20 European local pig breeds that may be useful for traceability purposes, for association studies and for breeding schemes. Population genetic analyses based on these candidate genes are able to uncover some clues regarding the hidden genetic substructure of these populations, as the extreme genetic closeness between Iberian and Alentejana breeds and an uneven admixture of the breeds studied. The results are in agreement with available knowledge regarding breed history and management, although largest panels of neutral markers should be employed to get a deeper understanding of the population’s structure and relationships.

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Section: Resultssupporting

confidence: 69%

Section: Resultsmentioning

confidence: 99%

Diversity across major and candidate genes in European local pig breeds

Muñoz¹,

Bozzi

García³

et al. 2018

PLoS ONE

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“…This single nucleotide polymorphism (SNP) has an allele (g.2228T) associated with a higher expression of the gene, which is fixed in our Iberian pigs but segregating in the Duroc ones. The SCD gene upregulation in the Iberian pigs may be associated with the higher desaturation potential of this breed [ 30 ], although, in our work, this difference in gene expression is not translated in a higher MUFA content in Iberian pig tissues. One explanation for this fact is the predominance of de novo lipogenesis over FA desaturation in the growing period analyzed here, which may lead to intense increases in SFA and to a dilution of the other FA.…”

Section: Discussionmentioning

confidence: 69%

Modulatory Effects of Breed, Feeding Status, and Diet on Adipogenic, Lipogenic, and Lipolytic Gene Expression in Growing Iberian and Duroc Pigs

Benítez¹,

Fernández²,

Isabel

et al. 2017

IJMS

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Meat quality depends on tissue composition which is in turn influenced by different factors, such as diet, genotype, age, or sex. We evaluated the effects of breed, 24 h fasting, and dietary energy source (HO: oleic acid versus CH: carbohydrates) on the expression of candidate genes involved in adipogenesis, lipogenesis, and lipolysis in the adipose tissue from Iberian and Duroc growing pigs. The Iberian pigs showed greater feed intake, backfat thickness, and saturated fatty acids (SFA) content in the subcutaneous fat, whereas the Duroc pigs had greater ham weight and polyunsaturated fatty acids (PUFA) content. In both breeds, the diet induced changes in the fatty acid (FA) composition of subcutaneous fat samples. The HO group had higher monounsaturated fatty acids (MUFA) and oleic acid, and lower SFA than the CH group. Regarding gene expression, breed and feeding status (fasting versus postprandial) had significant effects on gene expression, with quantitative interactions between them, while diet showed negligible effects. In general, adipogenic and lipogenic genes were upregulated in the Iberian pigs and in postprandial samples. In contrast, the expression of lipolytic genes showed complex interaction effects. Our results agree with the phenotypic differences between the Iberian and Duroc breeds and with the inhibition of lipogenesis by fasting. Quantitative interactions between breed and feeding status effects were observed, which indicates a different response to fasting of the two breeds, with the obese Iberian breed showing a more stable expression of lipogenic genes. These results highlight the complexity of lipid metabolism regulation, especially in relation to lipolysis processes.

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“…La enzima acetil-CoA carboxilasa (ACACA) junto con ACP y FASN, desempeñan un papel fundamental en el metabolismo de los ácidos grasos, catalizando la formación de malonil-CoA (acetil-ACP y malonil-ACP) a partir de acetil-CoA que, a su vez, actúa como intermediario en la síntesis de novo de los ácidos grasos de cadena larga Muñoz et al, 2007). La SCD se relaciona con esterificación de ácidos grasos; ACP, ACACA y FASN a lipogénesis, y SREBP1 como factor de transcripción y regulación de la expresión de diferentes genes lipogénicos (Benítez et al, 2018;Fernández et al, 2017;Li et al, 2020;Mohan et al, 2012;Wang et al, 2020). El cambio en la dieta puede modificar las proporciones de los lípidos, hasta llegar al punto de que la carne, pueda aportar un beneficio a la salud del consumidor (Duran-Montgé et al, 2009ª, 2009 ).…”

Section: Introductionunclassified

Efecto de dietas con harina de aguacate sobre lípidos en músculo, antioxidantes y expresión de genes en cerdos finalizados

Avalos

Lemus-Flores

Bugarin-Prado

et al. 2020

rbio

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El objetivo de esta investigación fue valorar el nivel de inclusión de harina de aguacate (HA) 0, 5 y 10% en la alimentación de cerdos por 56 días antes del sacrificio a 109±4 kg, sobre efectos en la modulación de los lípidos, presencia de antioxidantes en el Longissimus dorsi y expresión de genes en los tejidos Longissimus dorsi e hígado. Se emplearon en cada dieta ocho cerdos machos de 55±3 kg de peso inicial, para compararlas bajo un diseño completamente al azar, con análisis de varianza y prueba de Tukey. Los niveles de actividad antioxidante ABTS y DPPH, compuestos fenólicos totales y γ-tocoferol incrementan linealmente con inclusión de HA en la dieta, disminuyendo grasa intramuscular. La HA influye en incrementar el perfil de ácidos grasos poliinsaturados y la expresión de genes involucrados en el metabolismo de lípidos. La expresión alta en HA5 de genes lipogénicos (ACP, ACACA y FASN) se puede asociar al aumento de araquidónico (20:4 n6) y eicosapentanoico (20:3 n3), mientras que la esterificación de ácidos grasos (SCD) se puede asociar a la disminución de esteárico (18:0) y aumento de mayor relación 18:1/18:0 (9.66). Respecto a HA10 SREBP1 fue mayor en Longissimus dorsi, disminuyendo palmítico (16:0); aumentando: araquídico (20:0), linoleico (18:2 n6), PUFA, PUFA/SFA y PUFA/MUFA. Con HA5 aumenta el contenido de expresión de ACP, ACACA, FASN y SCD en ambos tejidos, siendo mayor en comparación con las dietas HA10.

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Validating porcine SCD haplotype effects on fatty acid desaturation and fat deposition in different genetic backgrounds

Cited by 12 publications

References 34 publications

Diversity across major and candidate genes in European local pig breeds

Diversity across major and candidate genes in European local pig breeds

Modulatory Effects of Breed, Feeding Status, and Diet on Adipogenic, Lipogenic, and Lipolytic Gene Expression in Growing Iberian and Duroc Pigs

Efecto de dietas con harina de aguacate sobre lípidos en músculo, antioxidantes y expresión de genes en cerdos finalizados

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