Validating tyrosinase homologue <i>Mel</i>A as a photoacoustic reporter gene for imaging<i> Escherichia coli</i>

Paproski, Robert J.; Li, Yan; Barber, Quinn; Lewis, John D.; Campbell, Robert E.; Zemp, Roger J.

doi:10.1117/12.2079638

Cited by 3 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tyr alone is sufficient for producing melanin in non-melanogenic cells from tyrosine without necessitating an extrinsic agent [236]. The tyrosinase homologue melA that produces melanin in bacteria has also been suggested [237]. Indeed, melanin is a dark and strongly absorbing pigment easily detectable with optoacoustic imaging systems.…”

Section: Dynamic Contrast Enhancement Approachesmentioning

confidence: 99%

Advanced optoacoustic methods for multiscale imaging of in vivo dynamics

et al. 2017

View full text Add to dashboard Cite

Visualization of dynamic functional and molecular events in an unperturbed in vivo environment is essential for understanding the complex biology of living organisms and of disease state and progression. To this end, optoacoustic (photoacoustic) sensing and imaging have demonstrated the exclusive capacity to maintain excellent optical contrast and high resolution in deep-tissue observations, far beyond the penetration limits of modern microscopy. Yet, the time domain is paramount for the observation and study of complex biological interactions that may be invisible in single snapshots of living systems. This review focuses on the recent advances in optoacoustic imaging assisted by smart molecular labeling and dynamic contrast enhancement approaches that enable new types of multiscale dynamic observations not attainable with other bio-imaging modalities. A wealth of investigated new research topics and clinical applications is further discussed, including imaging of large-scale brain activity patterns, volumetric visualization of moving organs and contrast agent kinetics, molecular imaging using targeted and genetically expressed labels, as well as three-dimensional handheld diagnostics of human subjects.

show abstract

Section: Dynamic Contrast Enhancement Approachesmentioning

confidence: 99%

Advanced optoacoustic methods for multiscale imaging of in vivo dynamics

et al. 2017

View full text Add to dashboard Cite

show abstract

“…However, Tyr is barely expressed in bacterial, causing the limitation on monitoring the bacterial infections or theranostic applications. To overcome this problem, Paproski et al 58 found the bacterial Tyr homologue melA, which could be a promising candidate as bacterial photoacoustic RG with strong°uorescence and luciferase activity.…”

Section: Tyrosinasementioning

confidence: 99%

Photoacoustic reporter genes for noninvasive molecular imaging and theranostics

Zhao

Zeng²,

Zhang

et al. 2020

J. Innov. Opt. Health Sci.

View full text Add to dashboard Cite

Noninvasive molecular imaging makes the observation and comprehensive understanding of complex biological processes possible. Photoacoustic imaging (PAI) is a fast evolving hybrid imaging technology enabling in vivo imaging with high sensitivity and spatial resolution in deep tissue. Among the various probes developed for PAI, genetically encoded reporters attracted increasing attention of researchers, which provide improved performance by acquiring images of a PAI reporter gene’s expression driven by disease-specific enhancers/promoters. Here, we present a brief overview of recent studies about the existing photoacoustic reporter genes (RGs) for noninvasive molecular imaging, such as the pigment enzyme reporters, fluorescent proteins and chromoproteins, photoswitchable proteins, including their properties and potential applications in theranostics. Furthermore, the challenges that PAI RGs face when applied to the clinical studies are also examined.

show abstract

“…Escherichia coli is a pathogenic bacteria transmitted by infected food. Many diseases caused by E. coli have been reported worldwide [10] Staphylococcus aureus is a gram-positive bacterium colonized on the skin, and present in the nose of 25% to 30% of healthy people [11]. It causes a range of skin infections, pneumonia, cardiovascular infections and other infections globally [12,13].…”

Section: Introductionmentioning

confidence: 99%

Effects of Helianthus annuus and Anthocleista djalonensis on Staphylococcus aureus and Escherichia coli

Ilori¹,

Okeke²,

Adeneye³

et al. 2022

JABB

View full text Add to dashboard Cite

Plant based medicines have been a part of traditional healthcare in most parts of the world for thousands of years. The medicinal plants are of great interest to human health. It has been proven that antimicrobials of plant origin work more efficiently with fewer side effects. The study aimed to identify the phytochemicals in the extracts of Helianthus annus and Anthocleista djalonensis and examine the effects of extracts of these plants on the growth of clinical bacterial isolates. Leaves of Helianthus annus and Anthocleista djalonensis were extracted in methanol, distilled water and hot water. The extract solution (100%) was further diluted to various concentrations (75%, 50%, and 25%). The data obtained were analyzed by Analysis of Variance (ANOVA) to determine significant (P< 0.05) effects. Significant differences between means were determined using Duncan’s Multiple Range Test (DMRT). The phytochemical screening indicated the presence of alkaloids, phenols, glycosides, flavonoids, terpenoids, saponins and quinone. The extracts of the plants inhibited the growth of the bacteria tested with varied effectiveness. The maximum antibacterial activities were observed in the aqueous extracts. Thus the extracts of Helianthus annus and Anthocleista djalonensis can be used in the development of new pharmaceuticals that address unmet therapeutic use.

show abstract

Validating tyrosinase homologue MelA as a photoacoustic reporter gene for imaging Escherichia coli

Cited by 3 publications

References 30 publications

Advanced optoacoustic methods for multiscale imaging of in vivo dynamics

Advanced optoacoustic methods for multiscale imaging of in vivo dynamics

Photoacoustic reporter genes for noninvasive molecular imaging and theranostics

Effects of Helianthus annuus and Anthocleista djalonensis on Staphylococcus aureus and Escherichia coli

Contact Info

Product

Resources

About