Validation and application of analytical method for glyphosate and glufosinate in foods by liquid chromatography-tandem mass spectrometry

Liao, Yang; Berthion, J. M.; Colet, Isabelle; Merlo, Mathilde; Nougadère, Alexandre; Hu, Renwei

doi:10.1016/j.chroma.2018.03.036

Cited by 75 publications

(46 citation statements)

References 21 publications

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“…The use of glyphosate causes a global contamination not only in the soil, the surface and underground waters, and the atmosphere, but also in human and animal foods and objects of common use, such as diapers, medical gauze, and feminine hygiene products (Torretta et al 2018;Zhao et al 2018). In food and beverage contamination, glyphosate residues was found in cereals-based products (Liao et al 2018), both conventional and organic honeys (Rubio et al 2013), in legumes (Çetin et al 2017), in beer (Jansons et al 2018) and wine and fruit juice (Zoller et al 2018). Glyphosate was then detectable in human milk (Torretta et al 2018) and urine (Conrad et al 2017;Connolly et al 2018).…”

Section: Glyphosate and Public Healthmentioning

confidence: 99%

Perinatal Exposure to Glyphosate and a Glyphosate-Based Herbicide Affect Spermatogenesis in Mice

Pham

Derian

Kervarrec

et al. 2019

Toxicological Sciences

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Glyphosate is the most widely used herbicide in the world. Several studies have investigated the effects of glyphosate and glyphosate-based-herbicides (GBHs) on male reproduction, but there is still little and conflicting evidence for its toxicity. In this study, we analyzed the effects of glyphosate, alone or in formula, on the male reproductive system. Pregnant mice were treated from E10.5 to 20 days postpartum (dpp) by adding glyphosate or a GBH (Roundup ® 3 Plus) to their drinking water at 0.5 (the acceptable daily intake, ADI dose), 5 and 50 mg/kg/day. Male offspring derived from treated mice were sacrificed at 5, 20 and 35 days-old (d.o.) and 8 monthsold (m.o.) for analysis. Our result showed that exposure to glyphosate, but not GBH, affect testis morphology in 20 d.o. and decrease serum testosterone concentrations in 35 d.o. males. We identified that the spermatozoa number decreased by 89% and 84% in 0.5 and 5 mg/kg/day of GBH and glyphosate groups, respectively. Moreover, the undifferentiated spermatogonia numbers were decreased by 60% in 5 mg/kg/day glyphosate group, which could be due to the alterations in the expression of genes involved in germ cell differentiation such as Sall4 and Nano3 and apoptosis as Bax and Bcl2. In 8 m.o. animals, a decreased testosterone level was observed in GBH groups. Our data demonstrate that glyphosate and GBHs could cause endocrine-disrupting effects on male reproduction at low doses. As glyphosate has effects at the ADI level, our data suggests that the current ADI for glyphosate could be overestimated.

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Section: Glyphosate and Public Healthmentioning

confidence: 99%

Perinatal Exposure to Glyphosate and a Glyphosate-Based Herbicide Affect Spermatogenesis in Mice

Pham

Derian

Kervarrec

et al. 2019

Toxicological Sciences

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“…A Chinese-French scientific paper in 2018 dealt the determination of GLYP and GLUF in 136 food samples, of which 34% of samples with high (banana, apple, orange, potato, carrot, and juice) and low (biscuits or bread) water contents and 66% of animal origin samples (milk-based foods included, e.g., milk, cheese, and butter) [58]. After a solvent extraction (acidified water, methanol, and dichloromethane), the authors performed a derivatization by means of FMOC and a solid phase extraction (SPE) C 18 for purifying and concentrating the extract and a HPLC-MS/MS analysis for determining the two compounds.…”

Section: Cereal Grainsmentioning

confidence: 99%

“…Finally, about the GLYP determination in cheese or, basically, in milk-based foods, the authors just discussed above the only paper present in the literature [58]. Please note that the milk as beverage will be discussed in other section.…”

Section: Meat Fish and Cheesementioning

confidence: 99%

A Review of the Analytical Methods Based on Chromatography for Analyzing Glyphosate in Foods

Avino¹,

Notardonato²,

Russo³

2020

Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production

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Glyphosate is a pesticide widely used in agriculture, horticulture, and silviculture as well as around homes and gardens. It was introduced by Monsanto in the early 1970s, and it is a broad spectrum, nonselective, post-emergence herbicide that inhibits plants' shikimic acid pathway. Glyphosate is considered as "difficult herbicide" in terms of trace analysis. It has low molecular weight, low volatility, thermal lability, and good water solubility. These properties cause problems in its extraction, purification, and detection. The determination often requires additional processes that may allow quantification by chromatographic methods. Several analytical procedures have been developed based on solid-phase extraction, ion-exchange chromatography, or matrix solid phase dispersion. Most published methods involve liquid extraction followed by clean-up. This review would like to revise the literature on this issue discussing the relevant chromatographic methods reported in the literature in terms of analytical parameters for analyzing such compound in food chain.

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“…Chromatography-mass spectrometry (LC/MS), or alternatively HPLC/MS, is the most common method to detect GLY in environmental samples due to its higher sensitivity (Liao et al 2018). Low analysis time has been achieved using solid-phase extractions with LC-SPE (Delmonico et al 2014), but with higher LOD.…”

Section: Chromatography-mass Spectrometrymentioning

confidence: 99%

“…It was reported that the LC/MS-MS method sufficiently detects GLY, but this method requires longer equilibration time, suffers from poor robustness and still has adverse impacts on column lifetime (Liao et al 2018). Kaczyński and Łozowicka compared LC/MS-MS and LC/FLD to detect traces of GLY in rapeseeds.…”

Section: Chromatography-mass Spectrometrymentioning

confidence: 99%

Nanocristais na Detecção de Glifosato

Valle¹

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Validation and application of analytical method for glyphosate and glufosinate in foods by liquid chromatography-tandem mass spectrometry

Cited by 75 publications

References 21 publications

Perinatal Exposure to Glyphosate and a Glyphosate-Based Herbicide Affect Spermatogenesis in Mice

Perinatal Exposure to Glyphosate and a Glyphosate-Based Herbicide Affect Spermatogenesis in Mice

A Review of the Analytical Methods Based on Chromatography for Analyzing Glyphosate in Foods

Nanocristais na Detecção de Glifosato

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