Validation and diagnostic efficacy of a lipase assay using the substrate 1,2‐o‐dilauryl‐rac‐glycero glutaric acid‐(6′methyl resorufin)‐ester for the diagnosis of acute pancreatitis in dogs

Abstract

On the basis of this study, the DGGR method is considered adequate for assaying serum lipase activity in dogs. The high sensitivity of the DGGR assay suggests it may be a useful screening test for canine pancreatitis.

Association between abdominal ultrasound findings, the specific canine pancreatic lipase assay, clinical severity indices, and clinical diagnosis in dogs with pancreatitis

“…A further potential limitation is the lack of histopathology in the cases evaluated. Despite histopathology being the historical gold standard, it has many limitations as previously discussed, and many recent studies have utilized a clinical gold standard for the diagnosis of pancreatitis …”

Comparison of measurements of 18 analytes in canine and feline blood samples using the in‐practice Falcor 350 and the reference KoneLab 30i analysers

“…The limits of agreement (mean of the differences ±2 sd) were too wide (−700 to 150 iu/l) for clinical reliability, and there were four outliers (192 versus 920, 185 versus 1104, 195 versus 1200 and 239 versus 1286 iu/l) for which sample quality, operator or instrument errors could not be found. The underestimation is almost certainly because of the different substrates (1,2‐O‐dilauryl‐rac‐glycero‐3‐glutaric acid‐(6′‐methylresorufin)‐ester [DGGR] versus 1,2 diglyceride [1,2 DiG]) used in the methodologies of the two analysers (Table 1) as it has been shown that in dogs, the DGGR‐based methods generate lower lipase activity results than the 1,2 DiG methods (Graca and others 2005).…”

“…The different substrates employed by the two methodologies can explain the poor correlation between the two analysers, while potential differences between species in the measurement of lipase activity using the DGGR substrate may explain the different correlations obtained for feline (r s =0·25) and canine (r s =0·88) samples in the present study. Species differences have been reported, as it has been shown that the lipase activity measured by the DGGR assay can be lower in healthy human beings than the activity measured by the same assay in clinically healthy dogs (Panteghini and others 2001, Graca and others 2005).…”

Diabetic Ketoacidosis

“…8-15); however, results are equipment and operator dependent. Preliminary studies evaluating a less expensive novel catalytic assay for colorimetric determination of serum lipase activity in dogs and cats found substantial agreement with SPEC cPL and SPEC fPL results from the same blood samples, suggesting that the novel catalytic assay may offer a cost-effective alternative diagnostic test for pancreatitis in dogs and cats (Graca et al, 2005;Oppliger et al, 2013). Measurement of canine and feline pancreatic-specific lipase (SPEC cPL and SPEC fPL) is currently the blood test of choice for identifying pancreatitis (Forman et al, 2004;Trivedi et al, 2011;McCord et al, 2012).…”