In recent literature, many researchers have highlighted that the feeling of loneliness can be considered a sign of relevant distress with short- and long-term consequences on the health of people who needs to be appropriately monitored and treated. In this view, the Interpersonal Acceptance–Rejection Loneliness Scale (IPARLS) has been developed to evaluate the subjective feelings and distress related to interpersonal loneliness and to overcome the limits of the previous scales; however, its psychometric characteristics have not yet been fully investigated. Starting from these considerations, two studies have been conducted. The first study, involving 688 adults (19–69 years; 51% females), assessed dimensionality, reliability, and validity of and tested the measurement invariance (MI) of the Italian version of the IPARLS. The second study, involving 205 adults (20–69 years; 51% females), investigated the relationship between loneliness and psychological adjustment. Results confirmed the unidimensionality of the scale and showed that it is reliable, valid, and fully invariant as a function of age and gender. Moreover, data showed a strong association between perceived loneliness and psychological adjustment. The IPARLS is confirmed to be a valid and reliable measure to investigate loneliness in the life cycle from clinical and research perspectives.