Validation of a direct-to-PCR COVID-19 detection protocol utilizing mechanical homogenization: A model for reducing resources needed for accurate testing

Abstract

Efficient and effective viral detection methodologies are a critical piece in the global response to COVID-19, with PCR-based nasopharyngeal and oropharyngeal swab testing serving as the current gold standard. With over 100 million confirmed cases globally, the supply chains supporting these PCR testing efforts are under a tremendous amount of stress, driving the need for innovative and accurate diagnostic solutions. Herein, the utility of a direct-to-PCR method of SARS-CoV-2 detection grounded in mechanical homogenization is examined for reducing resources needed for testing while maintaining a comparable sensitivity to the current gold standard workflow of nasopharyngeal and oropharyngeal swab testing. In a head-to-head comparison of 30 patient samples, this initial clinical validation study of the proposed homogenization-based workflow demonstrated significant agreeability with the current extraction-based method utilized while cutting the total resources needed in half.

Covid19predictor: Kli̇ni̇k Veri̇lere Ve Ruti̇n Testlere Dayali Olarak Covid-19 Teşhi̇si̇ İçi̇n Maki̇ne Öğreni̇mi̇ Modelleri̇ Geli̇şti̇rmeye Yarayan Web Tabanli Arayüz

“…Anahtar Kelimeler: Covid-19, Makine Öğrenmesi, Shiny, Arayüz For timely and accurate diagnosis, not only physicians but also nurses, paramedics and other healthcare professionals should have the ability to diagnose basic ECG rhythms [4]. The diagnosis of cardiac rhythms and initiation of appropriate treatment and intervention in the early period by paramedics, especially those providing service in the prehospital field, has an important position in reducing morbidity and mortality [5]. At the same time, correct interpretation of ECG can prevent unnecessary medical interventions [6].…”

“…While abdominal wall endometriosis (AWE) can be seen spontaneously, it usually develops secondary to a surgical operation such as a cesarean section (CS), hysterectomy, or laparoscopy [5][6][7]. ÖZ Amaç: Covid-19 salgını sağlıkla ilgili, sosyal, ekonomik ve bireysel etkiler nedeniyle birçok ülkenin birincil sağlık sorunu haline gelmiştir.…”

“…It is critical that we as a global community continue to commit to the development of testing strategies to assist in Covid-19 public health efforts in all areas of the world if we are going to implement successful pandemic mitigation efforts [5].…”

Fast, cheap and sensitive: Homogenizer-based RNA extraction free method for SARS-CoV-2 detection by RT-qPCR

“…All these extraction methods are time consuming and causes a delay in clinical diagnosis. Moreover, this RNA extraction step increases the cost of the analysis by using more reagents and disposable supplies ( Morehouse et al., 2021 ). One of the most conventional nucleic acid extraction methods is isolation by solid chromatography, commonly known as ARN extraction kit by columns, which traps genomic material by resins charged positively, followed by elution steps that yield a high-quality pure sample.…”

Comparison of mechanical homogenization versus enzymatic digestion sample preparation methodologies for SARS-CoV-2 detection in saliva for surveillance of variants of concern on the University of Tennessee campus in early 2021

“…In this study, we compare three qPCR based methodologies for SARS-CoV-2 diagnostics using human saliva samples collected on the University of Tennessee, Knoxville campus. Two of these methodologies, the SalivaDirect with Proteinase K and the KingFisher RNA Extraction method, are commercially available and were compared to the Omni Direct-to-PCR (dPCR) method [9][10][11]. This comparative evaluation provides insight into the ability of different workflows to accurately detect viral RNA within saliva samples.…”