Validation of a liquid chromatography–tandem mass spectrometry method for the simultaneous quantification of 11 (fluoro)quinolone antibiotics in swine kidney

Toussaint, B.; Bordin, Guy; Amaya, Janosi; Rodríguez, A. R.

doi:10.1016/s0021-9673(02)00948-2

Cited by 62 publications

(25 citation statements)

References 30 publications

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“…The quinolones were then eluted with MeOH, the extract was evaporated and reconstituted in 2 mL of mobile phase. Toussaint et al developed a method for isolating 11 quinolones from pig kidney [306]. Other groups have developed methods for isolating quinolone residues from animal tissues [307].…”

Section: Quinolonesmentioning

confidence: 99%

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

Kinsella

O’Mahony

Malone

et al. 2009

Journal of Chromatography A

182

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A comprehensive review is presented on the current trends in sample preparation for isolation of veterinary drugs and growth promotors from foods. The objective of the review is to firstly give an overview of the sample preparation techniques that are applied in field. The review will focus on new techniques and technologies, which improve efficiency and coverage of residues. The underlying theme to the paper is the developments that have been made in multi-residue methods and particularly multi-class methods for residues of licensed animal health products, which have been developed in the last couple of years. The role of multi-class methods is discussed and how they can be accommodated in future residue surveillance.

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Section: Quinolonesmentioning

confidence: 99%

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

Kinsella

O’Mahony

Malone

et al. 2009

Journal of Chromatography A

182

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“…Earlier methods used UV almost exclusively 8,9 but more recently fluorescence detection has been preferred 10,11,12,13 . On the other hand, LC with MS is also used at the international level since this technique has a higher sensitivity and selectivity and is particularly suited for multi-residue determination in complex matrices 14,5,6,7,8 . The present paper reports on the analytical method based on LC with fluorescence (LC-Fl) deetection and its validation according the scheme suggested by European Union guideline EC/657/2002.…”

Section: Introductionmentioning

confidence: 99%

Validation of a Liquid Chromatography-Fluorescence Spectrometric Method for the Quantification of Four Quinolones in Pig and Chicken Muscle Following the European Union Guideline Ec/657/2002

VASQUEZ-MARTINEZ¹,

R²,

Villa³

et al. 2011

J. Chil. Chem. Soc.

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Quinolones are a group of structurally related antibacterial compounds. Over the last years their use in animal production has been greatly increased. This increase can cause the resistance of bacteria. In order to protect human health the European Union (EU), as well as other countries, has established Maximum Residue Limits (MRL) for these antibacterial agents in several foods. Among these foods, pig and chicken muscle have different MRLs in the sub-ppm range.A liquid chromatography-fluorescence spectrometric method has been developed and validated for the simultaneous assessment of four of the most used quinolones in edible animal matrices production. Simultaneous quantification of enrofloxacin, ciprofloxacin, oxolinic acid and flumequine was achieved by a method involving an initial aqueous extraction step followed by a solid-liquid-phase extraction using reverse-phase columns (C-18) and a final injection of all quinolones in a single chromatographic run using HPLC-Fluorescence detection. Nalidixic acid was used as internal standard.In addition to the description of the analytical method developed for the quantification of the studied quinolones, the different validation parameters of this method according to Commission Decision EC /657/2002 of the EU are presented and discussed.

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“…Most of the above mentioned meth-ods use fluorescence detection [4,13,15,18] or fluorescence detection coupled either with a photodiode array detector [14] or with an MS detector [9]. Two methods involve the use of photodiode array detector [6,12], one method applies UV detection [11], and three apply mass spectrometry [5,7,10].…”

Section: Introductionmentioning

confidence: 99%

Development of an HPLC multi‐residue method for the determination of ten quinolones in bovine liver and porcine kidney according to the European Union Decision 2002/657/EC

Christodoulou

Samanidou²,

Papadoyannis

2008

J of Separation Science

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A sensitive multi-residue analytical method was developed for the determination of ten quinolones: enoxacin, ofloxacin, norfloxacin, ciprofloxacin, danofloxacin, enrofloxacin, sarafloxacin, oxolinic acid, nalidixic acid, and flumequine in bovine liver and porcine kidney. A simple liquid extraction step followed by a solid phase extraction clean up procedure was applied for the extraction of quinolones from liver and kidney tissues. Recoveries of the extraction varied between 82 and 88% for bovine liver and 92 and 95% for porcine kidney. Separation was performed on an ODS-3 PerfectSil Target (250 x 4 mm) 5 microm analytical column at 25 degrees C. The mobile phase consisted of a mixture of TFA 0.1%-CH(3)CN-CH(3)OH, delivered at a flow rate of 1.2 mL/min according to a gradient program. Elution of quinolones and the internal standard (caffeine, 7.5 ng/microL) was complete within 27 min. Photodiode array detection was used for monitoring the eluants at 275 and 255 nm. The method was fully validated according to the European Union Decision 2002/657/EC, determining linearity, selectivity, decision limit, detection capability, accuracy, and precision. The LODs of the specific method of quinolone determination in bovine liver varied between 3 and 7 microg/kg and in porcine kidney between 3 and 4 microg/kg.

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Validation of a liquid chromatography–tandem mass spectrometry method for the simultaneous quantification of 11 (fluoro)quinolone antibiotics in swine kidney

Cited by 62 publications

References 30 publications

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

Validation of a Liquid Chromatography-Fluorescence Spectrometric Method for the Quantification of Four Quinolones in Pig and Chicken Muscle Following the European Union Guideline Ec/657/2002

Development of an HPLC multi‐residue method for the determination of ten quinolones in bovine liver and porcine kidney according to the European Union Decision 2002/657/EC

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