Validation of a method for the targeted analysis of 96 drugs in hair by UPLC–MS/MS

Montesano, Camilla; Johansen, Sys Stybe; Nielsen, Marie Katrine Klose

doi:10.1016/j.jpba.2013.08.050

Cited by 74 publications

(43 citation statements)

References 47 publications

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“…Possibly, newer and more advanced techniques for hair testing (e.g., Montesano et al, 2014) could have yielded better detection and better concordance with self-report. However, these techniques were not offered by the laboratories we queried.at the time we planned the study.…”

Section: Discussionmentioning

confidence: 99%

Hair drug testing results and self-reported drug use among primary care patients with moderate-risk illicit drug use

Gryczynski

Schwartz

Mitchell

et al. 2014

Drug and Alcohol Dependence

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Background This study sought to examine the utility of hair testing as a research measure of drug use among individuals with moderate-risk drug use based on the internationally-validated Alcohol, Smoking, and Substance Involvement Screening Test (ASSIST). Methods This study is a secondary analysis using baseline data from a randomized trial of brief intervention for drug misuse, in which 360 adults with moderate-risk drug use were recruited from two community clinics in New Mexico, USA. The current study compared self-reported drug use on the ASSIST with laboratory analysis of hair samples using a standard commercially-available 5-panel test with assay screening and gas chromatography/mass spectrometry (GC/MS) confirmation. Both self-report and hair testing covered a 3 month period. Results Overall concordance between hair testing and self-report was 57.5% (marijuana), 86.5% (cocaine), 85.8% (amphetamines), and 74.3% (opioids). Specificity of hair testing at standard laboratory cut-offs exceeded 90% for all drugs, but sensitivity of hair testing relative to self-report was low, identifying only 52.3% (127/243) of self-disclosed marijuana users, 65.2% (30/46) of cocaine users, 24.2% (8/33) of amphetamine users, and 2.9% (2/68) of opioid users. Among participants who disclosed using marijuana or cocaine in the past 3 months, participants with a negative hair test tended to report lower-frequency use of those drugs (p< .001 for marijuana and cocaine). Conclusions Hair testing can be useful in studies with moderate-risk drug users, but the potential for under-identification of low-frequency use suggests that researchers should consider employing low detection cut-offs and using hair testing in conjunction with self-report.

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Section: Discussionmentioning

confidence: 99%

Hair drug testing results and self-reported drug use among primary care patients with moderate-risk illicit drug use

Gryczynski

Schwartz

Mitchell

et al. 2014

Drug and Alcohol Dependence

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“…Given the generalized consumption of caffeine, intake of a test dose would no longer be required in the case of a phenotyping procedure involving hair analysis. Caffeine has been determined in hair using a variety of extraction media and analytical techniques, such as GC-MS, LC-UV, ion mobility MS, LC-QTOF-MS and LC-MS/MS [15][16][17][18][19][20], although only one published method was fully validated for caffeine [19]. To the best of our knowledge, our study is the first to provide an optimized and validated method for the combined determination of caffeine and its metabolite paraxanthine in hair.…”

Section: Introductionmentioning

confidence: 99%

An optimized and validated SPE-LC–MS/MS method for the determination of caffeine and paraxanthine in hair

Kesel

Lambert

Stove

2015

Talanta

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Caffeine is the probe drug of choice to assess the phenotype of the drug metabolizing enzyme CYP1A2. Typically, molar concentration ratios of paraxanthine, caffeine's major metabolite, to its precursor are determined in plasma following administration of a caffeine test dose. The aim of this study was to develop and validate an LC-MS/MS method for the determination of caffeine and paraxanthine in hair. The different steps of a hair extraction procedure were thoroughly optimized. Following a three-step decontamination procedure, caffeine and paraxanthine were extracted from 20 mg of ground hair using a solution of protease type VIII in Tris buffer (pH 7.5). Resulting hair extracts were cleaned up on Strata-X ™ SPE cartridges.All samples were analyzed on a Waters Acquity UPLC ® system coupled to an AB SCIEX API 4000™ triple quadrupole mass spectrometer. The final method was fully validated based on international guidelines. Linear calibration lines for caffeine and paraxanthine ranged from 20 to 500 pg/mg. Precision (%RSD) and accuracy (%bias) were below 12 and 7 %, respectively. The isotopically labeled internal standards compensated for the ion suppression observed for both compounds. Relative matrix effects were below 15 %RSD. The recovery of the sample preparation procedure was high (> 85 %) and reproducible. Caffeine and paraxanthine were stable in hair for at least 644 days. The effect of the hair decontamination procedure was evaluated as well. Finally, the applicability of the developed procedure was demonstrated by determining caffeine and paraxanthine concentrations in hair samples of ten healthy volunteers. The optimized and validated method for determination of caffeine and paraxanthine in hair proved to be reliable and may serve to evaluate the potential of hair analysis for CYP1A2 phenotyping.

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“…Application of the developed method in such cases as described in the paper may 475 assess the health risk of a child and, in turn, the implementation of an appropriate therapeutic 476 intervention. The UPLC-MS/MS method was also developed for the determination of a wide group 492 of 96 drugs, such as BZD, opiates, amphetamines, hallucinogens, antihistamines, 493 antidepressants, antipsychotics, barbiturates and other sedatives, and muscle relaxants in hair 494 [83]. The low range of the LOD and LOQ, as well as a recovery value higher than 70%, and M a n u s c r i p t…”

mentioning

confidence: 99%

Analytical methodologies for the determination of benzodiazepines in biological samples

Persona

Madej

Knihnicki

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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Validation of a method for the targeted analysis of 96 drugs in hair by UPLC–MS/MS

Cited by 74 publications

References 47 publications

Hair drug testing results and self-reported drug use among primary care patients with moderate-risk illicit drug use

Hair drug testing results and self-reported drug use among primary care patients with moderate-risk illicit drug use

An optimized and validated SPE-LC–MS/MS method for the determination of caffeine and paraxanthine in hair

Analytical methodologies for the determination of benzodiazepines in biological samples

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