Validation of a method of high performance liquid chromatography with fluorescence detection for melamine determination in UHT whole bovine milk

Finete, Virgínia de Lourdes Mendes; Gouvêa, Marcos Martins; Marques, Flávia Ferreira de Carvalho; Netto, Annibal Duarte Pereira

doi:10.1016/j.foodcont.2014.12.001

Cited by 34 publications

(5 citation statements)

References 27 publications

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“…The flow rate was set at 0.6 mL/min, MEL retention times were 4.6 min (RSD= 1.21%). This result is entirely consistent with the previous works [17].…”

Section: Chromatographic and Ms Conditionssupporting

confidence: 94%

Validation of the Method for Determination of Melamine and Investigation its Trace in Milk from Vietnam by LC-MS/MS

Hieu-Tran

2021

AJACR

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This work describes a rapid, selective, and sensitive method by using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to detect melamine (MEL) in milk and dairy products. The optimal conditions of liquid chromatographic separation extraction and mass spectroscopy of MEL have also been examined. The linear range for analyte detected by the method was 0.5÷100.0 ng/mL, with correlation coefficients was 0.999. Mean recoveries of the method in the real samples at three spike levels (low, medium, and high) were within the range of 98.5% ÷102.5% (n =7). LOD, LOQ values of the method were 10 and 30 ng/mL, respectively. The influence of the matrix effect on the accuracy, repeatability, and recovery of the process was insignificant. The proposed method was used to quantify the content of this compound in various real samples, which were collected in Ho Chi Minh City-Vietnam in 2020.

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“…The flow rate was set at 0.6 mL/min, MEL retention times were 4.6 min (RSD= 1.21%). This result is entirely consistent with the previous works [17].…”

Section: Chromatographic and Ms Conditionssupporting

confidence: 94%

Validation of the Method for Determination of Melamine and Investigation its Trace in Milk from Vietnam by LC-MS/MS

Hieu-Tran

2021

AJACR

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“…The current literature includes numerous chromatographic techniques for detecting melamine in milk, but these methods are highly sophisticated and necessitate intricate pretreatment procedures prior to detection. − Some colorimetric approaches are also reported in literature; however, these methods entail a long pretreatment process and an extended sensing procedure, thereby demanding a substantial amount of chemicals for effective detection of the adulterant. ,− …”

Section: Introductionmentioning

confidence: 99%

One-Pot Interference-Based Colorimetric Detection of Melamine in Raw Milk via Green Tea-Modified Silver Nanostructures

Das,

Biswas,

Mazumder

2024

ACS Omega

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Detection of melamine has proven to be a challenge, requiring the use of complex analytical techniques. This study introduces an innovative, straightforward one-pot technique for qualitative assessment of the milk adulterant melamine. Originally, silver nanoparticles (AgNPs) were synthesized by utilizing green tea extract, which acted as both a reducing and sensing element. The synthesized AgNPs were characterized using UV–vis spectroscopy, X-ray diffraction, zeta potential, transmission electron microscopy, field emission scanning electron microscopy, and Fourier transform infrared spectroscopy. Melamine, rich in –NH2 groups, interacts with the biopolyphenols of green tea extract through hydrogen bonding. This interaction inhibits the formation of nanoparticles, resulting in a noticeable colorimetric response. The data obtained were confirmed by a standard UV–vis spectrophotometer and validated by the high-performance liquid chromatography technique. The limit of detection achieved by this scheme was quite low, falling below the permissible levels recommended by government bodies, e.g., the Food Safety and Standards Authority of India (FSSAI).

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“…Although recent work exploits new and elaborate techniques for the detection and analysis of melamine present in different matrices, research works for the improvement of conventional techniques continue also to develop nowadays [2][3][4][5][6][7][8]. Chromatographic separation methods are the most largely and commonly used methods for the analysis of melamine.…”

Section: Introductionmentioning

confidence: 99%

Thin Melamine Imprinted Sol Gel Coating on Silica Beads: Experimental and Statistical Physics Study

et al. 2018

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In this work, a surface molecular imprinted polymer was developed at the silica surface by the action of ethylene glycol dimethacrylate crosslinking agent with methacrylamide surface grafts. The produced adsorbents were tested for the adsorption of melamine. The adsorption mechanism of melamine was checked by means of adsorption kinetic measurements and inspection of the models governing the adsorption isotherm. The adsorption kinetics followed a pseudo-second order kinetic reaching a maximum adsorption of 2.16 mg g −1. Beside the known binding isotherm models of Langmuir and Freundlich, the adsorption isotherms were also inspected using a statistical physics model based on the energies of binding sites. It appeared that the experimental results were better described by this last approach. The variation in adsorption capacities recorded between low and high concentration ranges was related to the entropy value.

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Validation of a method of high performance liquid chromatography with fluorescence detection for melamine determination in UHT whole bovine milk

Cited by 34 publications

References 27 publications

Validation of the Method for Determination of Melamine and Investigation its Trace in Milk from Vietnam by LC-MS/MS

Validation of the Method for Determination of Melamine and Investigation its Trace in Milk from Vietnam by LC-MS/MS

One-Pot Interference-Based Colorimetric Detection of Melamine in Raw Milk via Green Tea-Modified Silver Nanostructures

Thin Melamine Imprinted Sol Gel Coating on Silica Beads: Experimental and Statistical Physics Study

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