Validation of a minimally invasive blood-sampling technique for the analysis of hormones in domestic rabbits, Oryctolagus cuniculus (Lagomorpha)

Voigt, Christian C.; Fassbender, M; Dehnhard, Martin; Wibbelt, Gudrun; Jewgenow, Katarina; Hofer, Heribert; Schaub, Günter A.

doi:10.1016/j.ygcen.2003.08.005

Cited by 45 publications

(39 citation statements)

References 17 publications

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“…Exact blood volumes can be determined by using the adequate bug size (Voigt et al 2004). Depending on the different body masses of the focus species, we used bugs of various larval stages to avoid collecting too much blood, which could lead to drastic loss of blood volumes, especially in small-sized primates.…”

Section: Discussionmentioning

confidence: 99%

“…Since each stage has a characteristic size (e.g. L1 = 0.4 cm, Imago = 4 cm), the quantity of blood required can be defined precisely from 0.1 to 4.0 ml (for details, see Voigt et al 2004). After the bug has recognised the victim, it punctures the skin with its proboscis, which is 32-fold smaller in diameter than a common 26-gauge needle, and then starts to suck blood.…”

Section: Methodsmentioning

confidence: 99%

“…When possible effects of the bug's haemolymph or saliva on the blood sample were studied, it was shown that the sample remains unchanged if it is redrawn from the bug's crop within approximately 30 min after the bug has finished its blood meal. The blood then can be used for a variety of analysis, including lymphocyte cultures (Volleth 1985), doubly-labelled water experiments (Voigt et al 2003), antibodies (Voigt et al 2006), determining the concentration of steroid hormones (Voigt et al 2004) and haematology (R. Thomsen and C.C. Voigt, in preparation).…”

Section: Introductionmentioning

confidence: 99%

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Non-invasive blood sampling from primates using laboratory-bred blood-sucking bugs (Dipetalogaster maximus; Reduviidae, Heteroptera)

Thomsen

Voigt

2006

Primates

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Primates are easily stressed by the conventional veterinary blood sampling routine and consequently, measured blood parameters may be biased. In this study, we tested blood-sucking bugs (Dipetalogaster maximus) on one lemur and two ape species (Microcebus murinus, Pongo abelii, Pan paniscus) as an alternative, non-invasive technique for bleeding primates. Within time periods of between 6 and 62 min we obtained blood volumes of 0.01-2.4 ml in 11 out of 12 trials from all three species. Therefore, we conclude that these bugs represent a new, gentle and effective tool for bleeding captive primates without stress.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Non-invasive blood sampling from primates using laboratory-bred blood-sucking bugs (Dipetalogaster maximus; Reduviidae, Heteroptera)

Thomsen

Voigt

2006

Primates

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“…In some species, the site is unique (e.g., in platypus, the vascular sinus on the anterior margin of the upper bill, Handasyde et al 2003; in penguins, the medial metatarsal vein, Sergent et al 2004; in bats, the interfemoral vein,, Wimsatt et al 2005) and in others, where access to veins is difficult, blood-sucking bugs (Reduviidae, Heteroptera) have been used to obtain a blood sample (Voight et al 2004;Arnold et al 2008). The need for restraint is critical and may require an anesthetic (an inhalant such as isoflurane or an injectable such as telazol), but for the smaller species, a simple handling bag that covers the eyes of the animal and minimizes stress may be sufficient.…”

Section: Sample Collection and Storagementioning

confidence: 99%

Measuring stress in wildlife: techniques for quantifying glucocorticoids

et al. 2011

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Stress responses play a key role in allowing animals to cope with change and challenge in the face of both environmental certainty and uncertainty. Measurement of glucocorticoid levels, key elements in the neuroendocrine stress axis, can give insight into an animal's well-being and can aid understanding ecological and evolutionary processes as well as conservation and management issues. We give an overview of the four main biological samples that have been utilized [blood, saliva, excreta (feces and urine), and integumentary structures (hair and feathers)], their advantages and disadvantages for use with wildlife, and some of the background and pitfalls that users must consider in interpreting their results. The matrix of choice will depend on the nature of the study and of the species, on whether one is examining the impact of acute versus chronic stressors, and on the degree of invasiveness that is possible or desirable. In some cases, more than one matrix can be measured to achieve the same ends. All require a significant degree of expertise, sometimes in obtaining the sample and always in extracting and analyzing the glucocorticoid or its metabolites. Glucocorticoid measurement is proving to be a powerful integrator of environmental stressors and of an animal's condition.

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“…It enabled us to obtain a blood sample from incubating females without having to trap them. This non-invasive method is gaining popularity for sampling sensitive, wild or captured animals [24][25][26][27][28][29][30]. Larvae of Dipetalogaster maxima, Uhler 1894 (Heteroptera, Reduviidae) were obtained from G. Schaub (Ruhr Universität Bochum, Germany).…”

Section: Blood Samplingmentioning

confidence: 99%

Development of New Microsatellite (STR) Markers for Montagu’s Harrier (Circus pygargus) via 454 Shot-Gun Pyrosequencing

Janowski¹,

Grohme²,

Frohme³

2014

TOOENIJ

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During the last decades the ground-breeding Montagu's harrier (Circus pygargus, Linnaeus, 1758) has changed its breeding habitats in Europe to agricultural areas in which many local populations would be close to extinction without a special nest protection regime. Although Montagu's harrier is a well-studied species in terms of ecology and breeding biology, its genetic structure and population genetics are almost unknown. As there is a lack of good genetic markers we developed a set of 19 microsatellite markers comprising 16 new STR markers which were identified by next-generation sequencing (NGS) using 454 shot-gun pyrosequencing of genomic DNA. The STR markers were arranged into three multiplex PCR sets for high throughput genotyping and characterised. The marker set provides a powerful tool for kinship analysis. The combined non-exclusion probability for parent pairs was 1.13*10 -11 . Only three loci showed PIC values < 0.50. In total, 121 known family relationships were compared with genetically calculated ones to test the markers suitability for parentage analysis. In 97.5% of all cases full-sibships were accurately determined and 97.6% of all mothers were assigned correctly to their chicks. The present multiplex PCR panels can be used to investigate several hypotheses concerning breeding behaviour, kinship, exchange rates between populations and phylogeography.

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Validation of a minimally invasive blood-sampling technique for the analysis of hormones in domestic rabbits, Oryctolagus cuniculus (Lagomorpha)

Cited by 45 publications

References 17 publications

Non-invasive blood sampling from primates using laboratory-bred blood-sucking bugs (Dipetalogaster maximus; Reduviidae, Heteroptera)

Non-invasive blood sampling from primates using laboratory-bred blood-sucking bugs (Dipetalogaster maximus; Reduviidae, Heteroptera)

Measuring stress in wildlife: techniques for quantifying glucocorticoids

Development of New Microsatellite (STR) Markers for Montagu’s Harrier (Circus pygargus) via 454 Shot-Gun Pyrosequencing

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