Validation of a multiplex flow immunoassay for detection of IgG antibodies against SARS-CoV-2 in dried blood spots

Turgeon, Coleman T.; Sanders, Karen A.; Rinaldo, Piero; Granger, Dane; Hilgart, Heather; Matern, Dietrich; Theel, Elitza S.

doi:10.1371/journal.pone.0252621

Cited by 16 publications

(13 citation statements)

References 20 publications

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“…For SARS-CoV-2 studies, adoption of DBS may reduce frontline worker demand, improve sampling in resource-limited areas, and increase study enrollment. Though prior work with DBS and plasma pairs has supported the suitability of DBS for SARS-CoV-2 antibody binding assays ( 27 – 36 ), important gaps remained that prevented the broad implementation of this sample type. For example, most studies used DBS with venous ( 27 , 30 ) or fingerstick blood collected at the clinic with the assistance of a phlebotomist ( 29 , 31 – 33 , 35 ), which does not account for variables introduced with self-sampling or ambient shipping.…”

Section: Discussionmentioning

confidence: 99%

“…Prior studies often elute DBS discs in volumes that prepare samples directly for testing in a single assay format ( 27 , 29 , 31 , 32 , 34 , 36 ). Because we sought to employ DBS for various assays, we first measured total IgG levels in DBS eluate and plasma pairs to estimate the volume of plasma extracted from each disc, to ultimately inform sample dilutions and data normalization approaches.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, when the current pandemic began, several groups sought to verify the use of DBS eluates for SARS-CoV-2 antibody assays. The most robust of these studies compared antibody binding measurements between paired DBS eluates and plasma or sera from COVID-19 convalescent individuals ( 27 – 36 ), and, impressively, all have found strong agreement between sample types. Despite this supportive evidence, the majority of these reports did not use self-collected, mailed-in fingerstick DBS cards and the compatibility of DBS with other assay formats, including neutralization, has not been carefully examined for SARS-CoV-2.…”

Section: Introductionmentioning

confidence: 98%

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SARS-CoV-2 Antibody Binding and Neutralization in Dried Blood Spot Eluates and Paired Plasma

et al. 2021

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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SARS-CoV-2 Antibody Binding and Neutralization in Dried Blood Spot Eluates and Paired Plasma

et al. 2021

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“…108 neg. Venous serum antibody measurements NPA: 98.0% PPA: 100% TA: 99.4% Imprecision 18 *Borderline measurements were treated as negative for ease of method comparison. † DBS samples were self-collected by the patient.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of SARS-CoV-2 antibodies using dried blood spot for at-home collection

Miesse

Collier

Grant

2022

Sci Rep

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The utilization of vaccines to fight the spread of SARS-CoV-2 has led to a growing need for expansive serological testing. To address this, an EUA approved immunoassay for detection of antibodies to SARS-CoV-2 in venous serum samples was investigated for use with dried blood spot (DBS) samples. Results from self-collected DBS samples demonstrated a 98.1% categorical agreement to venous serum with a correlation (R) of 0.9600 while professionally collected DBS samples demonstrated a categorical agreement of 100.0% with a correlation of 0.9888 to venous serum. Additional studies were performed to stress different aspects of at-home DBS collection, including shipping stability, effects of interferences, and other sample-specific robustness studies. These studies demonstrated a categorical agreement of at least 95.0% and a mean bias less than ± 20.0%. Furthermore, the ability to track antibody levels following vaccination with the BioNTech/Pfizer vaccine was demonstrated with serial self-collected DBS samples from pre-dose (Day 0) out to 19 weeks.

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“… 32 A flow immunoassay using magnetic beads for the detection of IgG antibodies against SARS-CoV-2 was also validated. 33 All diagnoses recently applied prove that this technology has been successful in detecting SARS-CoV-2 antibody. On the other hand, the serodiagnosis still needs improvement to guaranty appropriate sensitivity and specificity.…”

Section: Introductionmentioning

confidence: 99%

Peptide-Integrated Superparamagnetic Nanoparticles for the Identification of Epitopes from SARS-CoV-2 Spike and Nucleocapsid Proteins

Scussel

Feuser

Luiz

et al. 2022

ACS Appl. Nano Mater.

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The COVID-19 pandemic, caused by the fast transmission and spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is currently considered a serious health problem, requiring an effective strategy to contain SARS-CoV-2 dissemination. For this purpose, epitopes of the SARS-CoV-2 spike (S) and sucleocapsid (N) proteins were identified by bioinformatics tools, and peptides that mimic these epitopes were chemically synthesized and then conjugated to superparamagnetic nanoparticles (SPMNPs). Three peptides from S protein and three from N protein were used as antigens in a conventional enzyme-linked immunosorbent assay (ELISA) against serum samples from COVID-19-positive patients, or from healthy donors, collected before the pandemic. Three peptides were effective as antigens in conventional peptide-based ELISA, achieving 100% sensitivity and specificity, with high accuracy. The best-performing peptides, p2pS, p1pN, and p3pN, were associated with superparamagnetic nanoparticles (SPMNPs) and were used to perform nanomagnetic peptide-based ELISA. The p2pS–SPMNP conjugate presented 100% sensitivity and specificity and excellent accuracy (area under the curve (AUC) = 1.0). However, p1pN and p3pN peptides, when conjugated to SPMNPs, did not preserve the capacity to differentiate positive sera from negative sera in all tested samples, yet both presented sensitivity and specificity above 80% and high accuracy, AUC > 0.9. We obtained three peptides as advantageous antigens for serodiagnosis. These peptides, especially p2pS, showed promising results in a nanomagnetic peptide-based ELISA and may be suitable as a precoated antigen for commercial purposes, which would accelerate the diagnosis process.

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Validation of a multiplex flow immunoassay for detection of IgG antibodies against SARS-CoV-2 in dried blood spots

Cited by 16 publications

References 20 publications

SARS-CoV-2 Antibody Binding and Neutralization in Dried Blood Spot Eluates and Paired Plasma

SARS-CoV-2 Antibody Binding and Neutralization in Dried Blood Spot Eluates and Paired Plasma

Monitoring of SARS-CoV-2 antibodies using dried blood spot for at-home collection

Peptide-Integrated Superparamagnetic Nanoparticles for the Identification of Epitopes from SARS-CoV-2 Spike and Nucleocapsid Proteins

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