Validation of a novel ELISA for measurement of electronegative low-density lipoprotein

Faulin, Tanize do Espirito Santo; Sena, Karine Cavalcanti Maurício de; Telles, Andréia Elisa Rodrigues; Grosso, D.; Faulin, Edson José Bernardi; Abdalla, Dulcinéia Saes Parra

doi:10.1515/cclm.2008.333

Cited by 21 publications

(13 citation statements)

References 31 publications

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“…This limits their use for routine analysis of lipoprotein profiles. However, more reliable techniques for rapid analysis have recently been developed, including capillary electrophoresis and ELISA (Santo Faulin Tdo, 2008;Zhang, 2009;Zhang, 2008). These methods have confirmed previous data obtained by anion-exchange chromatography.…”

Section: Association Of Ldl(-) With Cardiovascular Risksupporting

confidence: 61%

Modified Forms of LDL in Plasma

Sánchez-Quesada¹,

Villegas²

2012

Atherogenesis

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Section: Association Of Ldl(-) With Cardiovascular Risksupporting

confidence: 61%

Modified Forms of LDL in Plasma

Sánchez-Quesada¹,

Villegas²

2012

Atherogenesis

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“…LDL(-) was obtained as described in Faulin et al [22]. Brieﬂy, the LDL fraction was separated by ultracentrifugation of human blood plasma from volunteers.…”

Section: Methodsmentioning

confidence: 99%

One-Year Conservative Care Using Sodium Bicarbonate Supplementation Is Associated with a Decrease in Electronegative LDL in Chronic Kidney Disease Patients: A Pilot Study

Rizzetto¹,

Mafra

Barra³

et al. 2017

Cardiorenal Med

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Background: Chronic kidney disease (CKD) patients develop metabolic acidosis when approaching stages 3 and 4, a period in which accelerated atherogenesis may ensue. Studies in vitro show that low pH may increase low-density lipoprotein (LDL) oxidation, suggesting a role for chronic metabolic acidosis in atherosclerosis. The present study attempted to evaluate the effects of conservative care using oral sodium bicarbonate (NaHCO₃) supplementation on the electronegative LDL [LDL(-)], a minimally oxidized LDL, plasma levels in CKD patients. Methods: Thirty-one CKD patients were followed by a multidisciplinary team during 15 months of care in which 1.0 mmol/kg/day oral NaHCO₃ supplementation was first given in the third month. Blood samples were collected 3 months before the initiation of oral NaHCO₃ supplementation (T1), at the time of the beginning of supplementation (T2), and thereafter, each 4 months (T3, T4 and T5) until month 15 of care. Blood parameters and LDL(-) were measured from these collections. Results: After 12 months of conservative care, creatinine clearance (MDRD) was kept stable, and serum bicarbonate (HCO₃^-) increased from 20.5 ± 2.9 to 22.6 ± 1.1 mM (p < 0.003). LDL(-) plasma levels declined from 4.5 ± 3.3 to 2.1 ± 0.9 U/L (p < 0.007) after reaching mean serum HCO₃^- levels of 22.6 ± 1.1 mM. Conclusions: Conservative care using oral NaHCO₃ supplementation was able to stabilize renal function and decrease serum levels of LDL(-), a modified proatherogenic lipoprotein, only when mean serum HCO₃^- levels approached 22 mM. This study constitutes evidence that alkali therapy, in addition to its beneficial effect on renal disease progression, might serve as a preventive strategy to attenuate atherogenesis in CKD patients.

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“…Changes in lipid profile were defined using the following criteria: total cholesterol ≥170 mg/dL; triacylglycerol ≥90 mg/dL; LDL-C ≥110 mg/dL and HDL-C <45 mg/dL [15]. Plasma concentrations of oxidized LDL (ox-LDL) were determined by ELISA (enzyme-linked immunosorbent assay), with reading in a spectrophotometer [16].…”

Section: Blood Collection and Biochemical Analysesmentioning

confidence: 99%

Polymorphisms in PPARG and APOE: relationships with lipid profile of adolescents with cardiovascular risk factors

Alves

Morais

Augusto

et al. 2017

Nutrire

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Background: Cardiovascular diseases constitute the main death cause worldwide resulting from a combination of genetic and lifestyle factors, and the prevalence among younger individuals has increased. It is important to early identify changes in lipid profile and the influence of genetic variations in specific genes on the individual patterns of lipid profile. Thus, the aim of this study was to verify the relationship of polymorphisms in PPAR-gamma gene (PPARG − rs1801282 − Pro12Ala) and in apolipoprotein E gene (APOE − rs429358 + rs7412, determinants of the APOE2, APOE3, or APOE4 genotypes) with lipid profile of adolescents under cardiovascular risk factors. Methods: This was a cross-sectional study with 115 adolescents aged 10-19 years, which presented cardiovascular risk factors. The students were evaluated regarding socioeconomic, anthropometric, biochemical, genetic, and dietetic variables. Student's t test or Mann-Whitney test were applied to the analysis of the genotypes. Multiple linear regression analysis was performed to determine the variables that most influenced the lipid profile. Results: Adolescents carrying PPARG Ala allele showed higher serum triglycerides (p = 0.0423) and very low-density lipoprotein (p = 0.0410) levels when compared to those carrying the wild genotype. For the APOE polymorphism, it was observed a trend of higher triglycerides (p = 0.0712) and very low-density lipoprotein (p = 0.0758) levels in the adolescents carrying the E4 allele when compared to those who did not carry this allele. Conclusion: The polymorphisms PPARG rs1801282 and APOE rs429358 + rs7412 seem to be related to the development of lipid profile alterations in adolescents.

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Validation of a novel ELISA for measurement of electronegative low-density lipoprotein

Abstract: This ELISA represents a very practical tool for measuring LDL(-) in human blood for widespread research and clinical sample use.

Cited by 21 publications

References 31 publications

Modified Forms of LDL in Plasma

Modified Forms of LDL in Plasma

One-Year Conservative Care Using Sodium Bicarbonate Supplementation Is Associated with a Decrease in Electronegative LDL in Chronic Kidney Disease Patients: A Pilot Study

Polymorphisms in PPARG and APOE: relationships with lipid profile of adolescents with cardiovascular risk factors

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