Validation of a PCR Assay for Chlamydophila abortus rRNA gene detection in a murine model

Silva-Zacarias, Francielle Gibson da; Alfieri, Amauri Alcindo; Spohr, Kledir Anderson Hofstaetter; Lima, Bruna Azevedo de Carvalho; Negrão, Fábio Juliano; Lunardi, Michele; Freitas, Júlio César de

doi:10.1590/s1516-89132009000700014

Cited by 2 publications

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“…In order to identify C. abortus in the evaluated specimens, a PCR assay that amplifies an 856-bp product from the intergenic region between the 16S and 23S rRNA genes was carried out according to Silva-Zacarias et al (2009a) o C. Additionally, the efficiencies of the nucleic acid extraction and DNA amplification procedures for the bovine abortion samples were evaluated by a PCR assay employing an internal control. These reactions were carried out with primers BOV1 (5'-ATACGCCTTCATTACCAG-3') and BOV2 (5'-TTGAATGGAGTAGTGCTG-3'), which amplify a 626-bp amplicon from the ND5 gene of bovine mitochondrial DNA (Wosiacki et al, 2005).…”

Section: Pcr Assaymentioning

confidence: 99%

Investigation of the possible role of Chlamydophila abortus in reproductive failures in nrazilian herds of domestic ruminants

Silva-Zacarias

Alfieri

Spohr

et al. 2009

Braz. arch. biol. technol.

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Section: Pcr Assaymentioning

confidence: 99%

Investigation of the possible role of Chlamydophila abortus in reproductive failures in nrazilian herds of domestic ruminants

Silva-Zacarias

Alfieri

Spohr

et al. 2009

Braz. arch. biol. technol.

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Recent biological techniques for diagnosis of Chlamydophila abortus in aborted goats

Osman

Goda

Mahmoud

et al. 2010

Journal of Veterinary Medical Research

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Chlamydophila abortus (C. abortus) is one of the most important causative agents of enzootic abortion which has been caused a serious economic problem in domesticated and wild ruminants world wide. This study was aimed to diagnose C. abortus infection in aborted goats in Ras Suder Research Station (South Sinai) -Desert Research Center from 2004-2006. Twenty aborted cases from 130 pregnant nannies were recorded and examined serologically using complement fixation test (CFT). Eighty percent (16/20) of the aborted cases were serologically positive and 20% (4/20) randomly collected from apparently healthy pregnant nannies were also had antibodies against C. abortus. Pathological lesions were detected. Ten aborted fetal samples from serologically positive aborted nannies were subjected to diagnosis using Polymerase Chain Reaction (PCR) showed positive results at 119 bp. According to this result, PCR proved to be feasible, reliable, specific and sensitive diagnostic tool in diagnosis of C. abortus infection. Beni-Suef Veterinary Medical Journal shedding at kidding. Intervention by the government and the private sector through farmer training and awareness campaigns is therefore recommended. A. (1990): Observation on the pathogenesis of Chlamydia psittaci infection in pregnant sheep. J. Comp. Pathol., 102: 221-237. Buxton, D.; Anderson, I. E.; Wattegdera, S. and Entrican, G. (2002): Ovine Chlamydial abortion characterization of the inflammatory immune response in placental tissues. J. of Comp. Pathology. 127: 2-3, 133-141. Chanton, G. H.; Thoma, R.; Corboz, L.; Borel, N. and Pospischil, A. (2002): Abortion in small ruminants in Switzerland: Investigations during two lambing seasons with special regard to chlamydia. SAT-Schweizer Archiv fur Tierheilkunde. 144: 9, 483-492. Alfieri,.; Lunardi, M. and Cesar, J. (2009): Validation of a PCR assay for Chlamydophila abortus rRNA gene detection in a murine model. Braz. Arch. Biol. Technol. V. 52 n. special: pp. 99-106. De Graves, F. J.; Gao, D.; Hehnen, H. R. and Kaltenboeck, B. (2003): Quantitive detection of Chlamydia psittaci and C. pecorum by high sensitivity realtime PCR reveals high prevalence of vaginal infection in cattle.J Clin Microbiol, 41, 1726-1729. De Graves, F. J.; Kim, T. Y.; Jee, J. B. and Hehnen H. R. (2004): Reinfection with Chlamydophila abortus by uterine and indirect cohort route reduces fertility in cattle pre-exposed to Chlamydophila. Infect. Immun. 72, 538-2545. Desouky, H. M.; Abd-El Razik, K. A and Ahmed Y. E. (2004): Diagnostic studies on ovine chlamydial abortion. J. Appl. Vet. Sci., NRC.1

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Validation of a PCR Assay for Chlamydophila abortus rRNA gene detection in a murine model

Cited by 2 publications

References 28 publications

Investigation of the possible role of Chlamydophila abortus in reproductive failures in nrazilian herds of domestic ruminants

Investigation of the possible role of Chlamydophila abortus in reproductive failures in nrazilian herds of domestic ruminants

Recent biological techniques for diagnosis of Chlamydophila abortus in aborted goats

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