Validation of a Pseudomonas aeruginosa porcine model of septic shock

Rimmelé, Thomas; Assadi, Abdulnasser; Benatir, Farida; Boselli, Emmanuel; Kaminski, Catherine N.; Arnal, Florence; Lambert, Charles Paul; Goudable, J.; Chassard, D.; Allaouchiche, Bernard

doi:10.1016/j.jinf.2005.10.023

Cited by 20 publications

(30 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous studies of the TNF-a, IL-1b and IL-6 levels in serum or plasma from experimentally infected pigs have been performed either by frequent measurements in shortterm trials (typically 6 h of observation) or by less frequent measurements in longer trials. An intravenous inoculation of Gram-negative bacteria or endotoxin generally induced a peak of 0.5 Â 10 4 -1 Â 10 5 ng L À1 TNF-a within 1 h (closely related to inoculation), followed within about 2 h by more moderate peaks of IL-1b (approximately 250 ng L À1 ) and IL-6 (1.2-7.0 mg L À1 ) (Hauptmann et al, 1994;Brix-Christensen et al, 2005;Rimmele et al, 2006;Castellheim et al, 2008;Ebdrup et al, 2008;Nielsen et al, 2009a). In shortterm trials with an intravenous inoculation of Gram-positive bacteria, one study reported a TNF-a peak to occur 3 h PI (approximately 40 ng L À1 ), one study demonstrated an IL-6 peak to occur 4 h PI and one study could not demonstrate any IL-6 in plasma at all (Ziegler-Heitbrock et al, 1992;Saetre et al, 2000;Nielsen et al, 2009b).…”

Section: Discussionmentioning

confidence: 99%

Intravenous inoculation of Staphylococcus aureus in pigs induces severe sepsis as indicated by increased hypercoagulability and hepatic dysfunction

Leifsson

Iburg

Jensen

et al. 2010

FEMS Microbiology Letters

View full text Add to dashboard Cite

Nine pigs were inoculated intravenously once or twice with 10(8) Staphylococcus aureus per kilogram body weight and sacrificed 12, 24 and 48 h after inoculation. Three sham-infected pigs served as controls. Blood samples were taken for bacteriology, haematology and clinical chemistry. A necropsy was carried out and tissue samples were collected for bacteriology and histology. The onset of clinical disease was seen at 7-8 h after inoculation. The blood bacterial counts remained low throughout the study. All infected pigs developed sepsis characterized by fever, neutrophilia, increased levels of C-reactive protein (CRP) and IL-6, and decreased levels of serum iron. The CRP and IL-6 levels peaked at 36 h, whereas IL-1beta and tumour necrosis factor-alpha showed no obvious changes. Thromboelastography showed increasing hypercoagulability from 12 h and onwards, whereas the platelet numbers declined slightly throughout the experiment. The levels of serum aspartate aminotransferase and bilirubin were elevated at 24 and 36 h. In conclusion, sepsis and severe sepsis were induced as evidenced by dysfunction of the blood clotting system and the liver.

show abstract

Section: Discussionmentioning

confidence: 99%

Intravenous inoculation of Staphylococcus aureus in pigs induces severe sepsis as indicated by increased hypercoagulability and hepatic dysfunction

Leifsson

Iburg

Jensen

et al. 2010

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…Various studies have validated bacterial injection as a sepsis model using a variety of bacteria. Injection of Pseudomonas aeruginosa [213] produced pigs with inflammatory cytokine profiles and a cardiovascular profile similar to human pathology, and continuous infusion of serogroup A streptococci leads to an acute septic shock with pulmonary hyperten-…”

Section: The Pig As a Model For The Study Of Infection And Inflammationmentioning

confidence: 99%

The mononuclear phagocyte system of the pig as a model for understanding human innate immunity and disease

Fairbairn

Kapétanovic

Sester

et al. 2011

Journal of Leukocyte Biology

165

144

View full text Add to dashboard Cite

The biology of cells of the mononuclear phagocyte system has been studied extensively in the mouse. Studies of the pig as an experimental model have commonly been consigned to specialist animal science journals. In this review, we consider some of the many ways in which the innate immune systems of humans differ from those of mice, the ways that pigs may address the shortcomings of mice as models for the study of macrophage differentiation and activation in vitro, and the biology of sepsis and other pathologies in the living animal. With the completion of the genome sequence and the characterization of many key regulators and markers, the pig has emerged as a tractable model of human innate immunity and disease that should address the limited, predictive value of rodents in preclinical studies.

show abstract

“…Sub-speciation of C. f. venerealis has been an area of some interest and there is an unusually high prevalence of C. f. venerealis biovar intermedius in South Africa [11]; however, the prevalence of this isolate has not been studied in Nigeria. Campylobacter f. fetus , found in the genital and intestinal tracts of cattle and sheep [2,10], is also a cause of infectious infertility in cattle and is also reported to cause a wide variety of invasive diseases in humans [12]. …”

Section: Introductionmentioning

confidence: 99%

Prevalence of bovine genital campylobacteriosis and trichomonosis of bulls in northern Nigeria

et al. 2013

View full text Add to dashboard Cite

BackgroundA survey was conducted to determine the prevalence of campylobacteriosis and trichomonosis, and their concurrence with brucellosis, in cattle in three states of northern Nigeria.MethodsA total of 602 preputial samples was collected from bulls in 250 herds and tested using culture and identification. Various indigenous and exotic breeds were studied and four major management systems were encountered. Age of the cattle was estimated using dentition, farm records or cornual rings.ResultsThe estimated true animal-level prevalence of Campylobacter fetus infection was 16.4% (95% CI: 13.0-20.7), of which 18.5% was C. f. fetus and 81.5% was C. f. venerealis. Of the latter, 92% were C. f. venerealis biovar intermedius strains. Animal-level prevalences in Adamawa, Kano and Kaduna states were 31.8%, 11.6% and 8.3% respectively, and were highest in bulls >7 years old (33.4%) and in the Gudali breed (28.8%). Of the 250 herds, 78 (25.5%, 95% CI: 19.4-32.7) had at least one infected bull, and herd prevalence was highest in the pastoral management system (43.5%). After adjustment for confounding using multivariable analysis, the odds of C. fetus infection were highest in Adamawa state (P < 0.01), in the pastoral management system (P < 0.01), and in bulls >7 years old (P = 0.01), and tended to be higher in Bos taurus breeds (P = 0.06). There was a strong positive association between the presence of campylobacteriosis and brucellosis (P < 0.01), both within bulls (OR = 8.3) and within herds (OR = 16.0). Trichomonosis was not detected in any herds.ConclusionBovine genital campylobacteriosis is prevalent particularly in the pastoral management system in northern Nigeria, with C. f. venerealis biovar intermedius as the major aetiology. There was a strong positive correlation between the occurrence of campylobacteriosis and brucellosis. No evidence of trichomonosis was found in herds in this study.

show abstract

Validation of a Pseudomonas aeruginosa porcine model of septic shock

Cited by 20 publications

References 27 publications

Intravenous inoculation of Staphylococcus aureus in pigs induces severe sepsis as indicated by increased hypercoagulability and hepatic dysfunction

Intravenous inoculation of Staphylococcus aureus in pigs induces severe sepsis as indicated by increased hypercoagulability and hepatic dysfunction

The mononuclear phagocyte system of the pig as a model for understanding human innate immunity and disease

Prevalence of bovine genital campylobacteriosis and trichomonosis of bulls in northern Nigeria

Contact Info

Product

Resources

About