Validation of a rapid and sensitive high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) assay for the simultaneous determination of existing and new antiretroviral compounds

Else, Laura; Watson, Victoria; Tjia, John; Hughes, Andrew T.; Siccardi, Marco; Khoo, Saye; Back, David

doi:10.1016/j.jchromb.2010.03.036

Cited by 123 publications

(111 citation statements)

References 57 publications

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“…Maraviroc concentrations were measured in CSF samples at the University of Liverpool, UK, using high-performance liquid chromatography-tandem mass spectrometry. 17 Analysis models MR spectra were analysed and quantified using two programs: a java-based version of the MR user interface package (jMRUI v. 5.0) 18 and the totally automatic robust quantitation in nuclear MR (TARQUIN) algorithm. 19 These were chosen because jMRUI has been used previously by our group and others to quantify CMRs in HIV disease.…”

Section: Subject Selectionmentioning

confidence: 99%

A comparison of two post-processing analysis methods to quantify cerebral metabolites measuredviaproton magnetic resonance spectroscopy in HIV disease

Scott¹,

Underwood²,

Garvey³

et al. 2016

BJR

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Objective: Non-invasive biomarkers to monitor cerebral function in treated human immunodeficiency virus (HIV) disease are required. Cerebral metabolite ratios (CMRs) measured by proton-MR spectroscopy ( 1 H-MRS) are a potential biomarker. Here, we compare two postprocessing software packages to quantify CMRs. Methods: Cerebral 1 H-MRS data from 11 HIV-positive subjects before and after antiretroviral therapy intensification with maraviroc were quantified using a java-based version of the MR user interface package (jMRUI) and the totally automatic robust quantitation in nuclear MR (TARQUIN). 1 H-MRS data included N-acetylaspartate (NAA), creatine (Cr), choline (Cho) and myo-inositol (mI) from three cerebral locations. Differences in quantification and clinical associations of CMRs measured by the two packages were evaluated. Results: Mean CMRs were generally lower when measured by TARQUIN than by jMRUI (NAA/Cr, Cho/Cr, mI/ Cr ratios of 1.78, 0.83, 0.81 for jMRUI, and 1.27, 0.25, 0.81 for TARQUIN). Longitudinal changes were observed in CMRs in the basal ganglia voxel although these changes were not statistically significant [17.1% (p 5 0.18), 10.0% (p 5 0.91) and 26.6% (p 5 0.61) and 114.8% (p 5 0.18), 1 17.9% (p 5 0.07) and 134.8% (p 5 0.17) for NAA/Cr, Cho/Cr and mI/Cr ratios measured by TARQUIN and jMRUI, respectively]. Plasma maraviroc concentration was associated with a decrease in mI/Cr ratio measured via TARQUIN (p 5 0.049). Conclusion: Although CMRs differed when quantified by jMRUI vs TARQUIN, these differences were consistently observed across three cerebral locations, and clinical associations were evident by both methods. Advances in knowledge: TARQUIN and jMRUI are viable options to use in the post-processing of cerebral MRS data acquired in HIV disease.

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Section: Subject Selectionmentioning

confidence: 99%

A comparison of two post-processing analysis methods to quantify cerebral metabolites measuredviaproton magnetic resonance spectroscopy in HIV disease

Scott¹,

Underwood²,

Garvey³

et al. 2016

BJR

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“…It was found the HPLC-MS method for simultaneous detection of darunavir and other antiretroviral drugs in plasma [2,[24][25][26][27][28] and in blood, saliva and tissue [29].…”

Section: Methods Of Analysismentioning

confidence: 99%

“…Triple-quadruple mass spectrometer, positive ionization mode, selective reaction monitoring (SRM). Plasma [28] HPLC-MS Phenomenex Gemini C18 column (2x150 mm) with a Phenomenex Security guard Gemini C18 pre column (2x4.0 mm). Gradient [700mL of methanol with 440mL of 10 mM acetic acid and 860mL of 10 mM ammonium acetate solution in water:methanol 85:15 and 15:85, v/v] was used as mobile phase.…”

Section: Ultraviolet In 205 Nmmentioning

confidence: 99%

Characteristics, Complexation and Analytical Methods of Darunavir

Kogawa

2014

BJPR

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What is it? Darunavir is a protease inhibitor used in the treatment of HIV infection. It is an important drug of therapy cocktail for patients infected with the virus. On the market there are darunavir ethanolate tablets of 75, 150, 300, 400, 600 and 800mg, because this is the most stable form. It is commercialized by Janssen-Cilag with the name Prezista TM . Why we started? This drug has low water solubility and poor bioavailability, therefore requires administration in doses relatively high to the success of the therapeutic effect. The complexation of drugs by using cyclodextrin is welcome in this respect to improve the solubility and hence increase the dissolution rate of poorly soluble drugs. A monograph about this compound has not been described, thus it is an extremely important quality control of darunavir to demonstrate its effectiveness and safety. What we did? Some existing analytical techniques have been discussed in this manuscript, focusing on bioanalytical and pharmaceutical quality control applications. What we found? This review showed the published analytical methods reported for the determination of darunavir and discuss about its characteristics and complexation with cyclodextrin.

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“…Rilpivirine hydrochloride is not yet official in I.P and B.P. A thorough literature survey revealed that UV spectroscopy [4][5][6] , HPLC [7][8][9] method for rilpivirine hydrochloride with combination of other drugs, UPLC 10 , LC-MS 11 , for its estimation in bulk, pharmaceutical dosage forms and biological samples. RESEARCH ARTICLE Figure 1.…”

Section: Rilpivirine Hydrochloride Is a Di-amino Pyrimidine Derivativmentioning

confidence: 99%

UV-Visible Spectrophotometric Method for the Estimation of Rilpivirine Hydrochloride in Pharmaceutical Dosage Form by Using Multivariate Technique

2015

Chem Sci Trans

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Abstract:To develop a simple, precise and accurate UV-method with multivariate calibration technique for estimation of rilpivirine hydrochloride in pharmaceutical dosage form. This technique is based on the use of the linear regression equations by using relationship between concentration and absorbance at five different wavelengths like 301, 303, 305, 307 and 309 nm. The results were treated statistically and were found highly accurate, precise and reproducible. The rilpivirine hydrochloride shows absorption maxima at 305 nm and obeyed Beer's law in the range of 0.5-3.0 µg/mL. The % recovery of tablets was found to be in the range of 101.1-101.7%. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.358 and 1.086 µg/mL, respectively. The low % RSD values are indicates the accuracy and precise of the method. The proposed methods can be successfully applied for method development, validation and multivariate analysis of rilpivirine hydrochloride.

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Validation of a rapid and sensitive high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) assay for the simultaneous determination of existing and new antiretroviral compounds

Cited by 123 publications

References 57 publications

A comparison of two post-processing analysis methods to quantify cerebral metabolites measuredviaproton magnetic resonance spectroscopy in HIV disease

A comparison of two post-processing analysis methods to quantify cerebral metabolites measuredviaproton magnetic resonance spectroscopy in HIV disease

Characteristics, Complexation and Analytical Methods of Darunavir

UV-Visible Spectrophotometric Method for the Estimation of Rilpivirine Hydrochloride in Pharmaceutical Dosage Form by Using Multivariate Technique

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