Validation of a real-time PCR assay for detection of swinepox virus

Souza, Felipe Augusto de; Júnior, Erlânio Marcelo Dos Santos; Laguardia-Nascimento, Mateus; Freitas, Tânia Rosária Pereira; Damaso, Clarissa R.; Júnior, Anselmo Vasconcelos Rivetti; Camargos, Marcelo Fernandes; Júnior, Antônio Augusto Fonseca

doi:10.1007/s00705-019-04403-w

Cited by 5 publications

(2 citation statements)

References 18 publications

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“…Of the The qPCR is a method that uses fluorescent chemicals to determine the total number of products after each round of PCR amplification. Compared with traditional PCR methods, qPCR has higher sensitivity, is simpler and takes less time [22]. In this study, a novel primer set and probe were designed for DuCV detection, and our qPCR assay was successfully employed to identify DuCV-positive field samples.…”

Section: Discussionmentioning

confidence: 99%

Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2

et al. 2021

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In this study, we developed a real-time quantitative polymerase chain reaction (qPCR) assay based on a dual-labeled hydrolysis probe to simultaneously detect both duck circovirus (DuCV) 1 and DuCV-2. The reproducibility, sensitivity and specificity of the primer set and probe were evaluated using other duck pathogens. The detection limit was 20 copies per µL. The intra-assay coefficients of variation (CVs) were ≤ 0.73% and the inter-assay CVs were ≤ 1.89%. No cross-reaction occurred with other duck pathogens. In addition, the qPCR assay was successfully applied to the simultaneous detection of DuCV-1 and DuCV-2 in clinical field samples. Therefore, this assay will be useful for laboratory diagnosis and epidemiological field studies of DuCV. Supplementary Information The online version contains supplementary material available at 10.1007/s11262-021-01862-9.

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Section: Discussionmentioning

confidence: 99%

Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2

et al. 2021

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“…A SWPV-specific real-time quantitative PCR (qPCR) was performed to confirm SWPV infection in clinical samples using probe and primer pair sequences targeting the C20L-C1L region [41]. The oligonucleotide sequences used were 5 -TAATCCGGGCATCAATCCTC-3 (forward primer), 5 GCTGATTGGGCCAGAAAATG-3 (reverse primer) and 5 FAM TTCCCTCCACAGCTGCAAATGCTACT-TAMRA-3 (probe).…”

Section: Detection Of Swpv-specific Sequences Using Pcr and Qpcrmentioning

confidence: 99%

Swinepox Virus Strains Isolated from Domestic Pigs and Wild Boar in Germany Display Altered Coding Capacity in the Terminal Genome Region Encoding for Species-Specific Genes

Kaiser

Wiedemann

Kühl³

et al. 2021

Viruses

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Swinepox virus (SWPV) is a globally distributed swine pathogen that causes sporadic cases of an acute poxvirus infection in domesticated pigs, characterized by the development of a pathognomonic proliferative dermatitis and secondary ulcerations. More severe disease with higher levels of morbidity and mortality is observed in congenitally SWPV-infected neonatal piglets. In this study, we investigated the evolutionary origins of SWPV strains isolated from domestic pigs and wild boar. Analysis of whole genome sequences of SWPV showed that at least two different virus strains are currently circulating in Germany. These were more closely related to a previously characterized North American SWPV strain than to a more recent Indian SWPV strain and showed a variation in the SWPV-specific genome region. A single nucleotide deletion in the wild boar (wb) SWPV strain leads to the fusion of the SPV019 and SPV020 open reading frames (ORFs) and encodes a new hypothetical 113 aa protein (SPVwb020-019). In addition, the domestic pig (dp) SWPV genome contained a novel ORF downstream of SPVdp020, which encodes a new hypothetical 71aa protein (SPVdp020a). In summary, we show that SWPV strains with altered coding capacity in the SWPV specific genome region are circulating in domestic pig and wild boar populations in Germany.

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