Validation of a spectrophotometric methodology for the quantification of polysaccharides from roots of Operculina macrocarpa (jalapa)

Abstract

Total polysaccharide content Spectrophotometry Validation A B S T R A C TThe roots from Operculina macrocarpa (L.) Urb., Convolvulaceae, are widely used in Brazilian traditional medicine as a laxative and purgative. The biological properties of this drug material have been attributed to its polysaccharides content. Thus, the aim of this study was to evaluate the polysaccharide content in drug material from O. macrocarpa by spectrophotometric quantitative analysis. The root was used as plant material and the botanical identification was performed by macro and microscopic analysis. The plant material was used to validate the spectrophotometric procedures at 490 nm for the quantification of the reaction product from drug polysaccharides and phenol-sulfuric acid solution. The analytical procedure was evaluated in order to comply with the necessary legal requirements by the determination of the following parameters: specificity, linearity, selectivity, precision, accuracy and robustness. This study provides with a simple and valid analytical procedure (linear, precise, accurate and reproducible), which can be satisfactorily used for quality control and standardization of herbal drug from O. macrocarpa.

Character and laxative activity of polysaccharides isolated from Dendrobium officinale

“…Indeed, the use of herbal remedies, particularly from dietary sources in the treatment and prevention of constipation, has been a common practice in many countries (including China) for many years. Research indicates that polysaccharides from herbal medicine exerted laxative effects, e.g., polysaccharides from Inula japonica and Operculina macrocarpa (Galvao et al, 2014;Shan et al, 2010). In addition, psyllium polysaccharides from Plantago ovate have previously been approved by FDA as therapeutic fibre supplements for the treatment of constipation (American College of Gastroenterology Chronic Constipation Task D. officinale is a famous Chinese functional and medicinal herb frequently used for gastrointestinal diseases, with polysaccharides as the major active constituents.…”

Spectrophotometric quantification of flavonoids in herbal material, crude extract, and fractions from leaves of Eugenia uniflora Linn

“…This approach is most appropriate way for analysis of biological matrices whose analyte-free matrix is not available. [ 36 ]…”

Analytical techniques and herbal Medicinal Plants: A Review of past and present techniques

“…In quantitative applications, the analysis and chemical composition of glucoside in walnuts were examined (Daglish, 1950). Several Spectrophotometric methods have been extensively utilized for the standardization and quantitative determination of a variety of biologically active compounds such as flavonoids, fundamental oils, phenolic compounds, and tannins) (Galvão et al 2014;Grubesić et al 2005;Aisha et al 2013;Bokov 2018;da Silva et al 2015;Alekandrovna et al2019). Essential nutrient elements were analyzed by the Indian researchers through AAS in a folk medicinal plant (Eucalyptus Oblique and Gava) (Santosh Teerthe 2018; Singh, et al 1997).…”