Validation of an analytical method using UPLC–MS/MS to quantify four bioactive components in rat plasma and its application to pharmacokinetic study of traditional and dispensing granules decoction of Ziziphi Spinosae Semen

Abstract

A rapid and sensitive UPLC–MS/MS method was established for the simultaneous quantification of 6′′′‐feruloylspinosin, spinosin, jujuboside A, and jujuboside B in rat plasma after the oral administration of traditional and dispensing granules (DG) decoction of Ziziphi Spinosae Semen (ZSS). The four components were separated using 0.1% formic acid and acetonitrile as a mobile phase by gradient elution at a flow rate of 0.3 mL/min equipped with a C18 column (2.1 × 50 mm, 1.7 μm particle size, Acquity BEH C18). The mass spectrometer was operated under multiple reaction monitoring mode. An aliquot of 100 μL rat plasma was deproteinized by 300 μL methanol. The supernatant was injected into the UPLC–MS/MS system for analysis. The calibration curves displayed good linearity. The intra‐day and inter‐day precisions (RSD) were less than 7.3%. The accuracies ranged from −1.3 to 6.1%. The extraction recoveries ranged from 95.8 to 101.9%, and the matrix effects were satisfactory. For DG, half‐life values (t1/2) of 6′′′‐feruloylspinosin and Cmax of jujuboside A were elevated remarkably. MRT0–t of jujuboside B was significantly increased. No significant variation was observed for the pharmacokinetic parameters of spinosin. The results could provide a scientific basis for the clinical application of traditional and DG decoction of ZSS.

The Hypoglycemic Activities and Underlying Mechanisms of Two Saponins‐Rich Components from Fried Ziziphus jujuba Mill. Kernel

“…Besides, there are other literatures showed the hypoglycemic activities of jujuboside A. [44,45] However, recently studies of the jujuboside B are focused on the gastrointestinal absorption and metabolic dynamics properties, [46,47] antitumor, [48,49] and anti-asthma [50] activities, little research reported the hypoglycemic activities of jujuboside B.…”

Quality consistency evaluation of chemical composition and pharmacology of Shaoyao‐Gancao decoction dispensing granules and traditional decoction

“…However, these studies have certain limitations. Existing research has primarily focused on evaluating the consistency of formula granule decoction and TD by assessing the similarity of chemical components (Chen et al, 2020; Lu et al, 2019; Lv et al, 2021; Ma et al, 2023; Tao et al, 2019; Zhen et al, 2021) or the equivalence of pharmacological effects (Dai & Wang, 2016; Yan et al, 2014). Few studies have combined chemical similarity and bioequivalence assessments to comprehensively evaluate the consistency between the two decoctions, which is necessary for a comprehensive analysis.…”