Validation of an analytical method for human plasma free amino acids by high-performance liquid chromatography ionization mass spectrometry using automated precolumn derivatization

Yoshida, Hiroo; Kondo, Kazuhiro; Yamamoto, Hiroyuki; Kageyama, Naoko; Ozawa, Shinobu; Shimbo, Kazutaka; Muramatsu, Toshiya; Imaizumi, Akira; Mizukoshi, Toshimi; Masuda, Junichi; Nakayama, Daisuke; Hayakawa, Yoshihiro; Watanabe, Kyoko; Mukaibatake, Kazuo; Miyano, Hiroshi

doi:10.1016/j.jchromb.2015.05.029

Cited by 55 publications

(44 citation statements)

References 29 publications

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“…Figure 2(b) shows the chromatogram of the human plasma sample under the optimized conditions. Amino acid concentrations in the human plasma samples were in the range 19.2-331 μM, which is quite similar with that described in the previous reports [16,17].…”

Section: Application To Human Plasma Samplessupporting

confidence: 90%

Amino Acid Analysis Using a Cartridge-Type Monolithic Silica Column

2017

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In this study, the application of a new type of monolithic silica column to the analysis of amino acids in human plasma samples has been investigated. The monolithic silica column (3.0 × 150 mm, MonoTower C18) provided good separation of the amino acids derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). Although the separation ability of this column was not much improved in comparison with the previous monolithic silica columns, it was similar with that of a 3 μm particle packed column. The column with length 150 mm had the same separation property as a tandem connection of columns of lengths 50 mm and 100 mm. After 1000 injections of human plasma samples, the peak widths for the NBD-amino acids were increased by only 8%. Thus, the newly developed monolithic silica column can be potentially applied for faster analysis and/or more efficient separation of biological compounds.

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Section: Application To Human Plasma Samplessupporting

confidence: 90%

Amino Acid Analysis Using a Cartridge-Type Monolithic Silica Column

2017

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“…The plasma samples were deproteinized using acetonitrile at a final concentration of 80% before the measurements. The concentrations of the plasma amino acids and their related metabolites were measured using high‐performance liquid chromatography‐electrospray ionization mass spectrometry, followed by pre‐column derivatization using previously described analytical methods …”

Section: Methodsmentioning

confidence: 99%

“…The concentrations of the plasma amino acids and their related metabolites were measured using high-performance liquid chromatography-electrospray ionization mass spectrometry, followed by pre-column derivatization using previously described analytical methods. [21][22][23]…”

Section: Geriatric Depressive Symptomsmentioning

confidence: 99%

Association between plasma α‐aminobutyric acid and depressive symptoms in older community‐dwelling adults in Japan

Adachi

Toyoshima

Nishimoto

et al. 2018

Geriatrics Gerontology Int

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Aim To examine the association between depressive symptoms and plasma amino acid related metaboli in older adults. Methods A total of 152 older adults aged ≥65 years, residing in Niigata, Japan, were used for analysis. We evaluated depressive symptoms using the Geriatric Depression Scale‐15, which has been validated in older community‐dwelling individuals, and used a cut off score of ≥5 to classify participants as having depressive symptoms. We used high‐performance liquid chromatography‐electrospray ionization mass spectrometry to measure the concentrations of plasma amino acid‐related metabolites, and carried out logistic regression analysis to assess the association between depressive symptoms and plasma amino acid‐related metabolites. Results Of the 119 older adults (mean age 76.3 years) included in the analysis, 22 were classified as having depressive symptoms (depressive group). There were no significant differences in physical and cognitive impairments between participants in the depressive and non‐depressive groups. The plasma α‐aminobutyric acid (AABA) level was significantly lower in the depressive group than in the non‐depressive group (P < 0.001). Logistic regression analysis showed the best‐fit model, which included AABA, leucine, threonine, hydroxyl proline and histidine levels (area under the receiver operating characteristic curve 0.8346; 95% confidence interval 0.7365–0.9326). In particular, the plasma AABA level was strongly associated with depressive symptoms. Conclusions Plasma AABA level is significantly associated with depression symptoms in older community‐dwelling adults in Japan. Thus, plasma AABA might serve as a potential marker of depression in older adults aged ≥65 years. Geriatr Gerontol Int 2019; 19: 254–258.

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“…CE has been extensively used for their analysis in different matrices, with different detection systems, such as indirect UV [20,21], conductivity [22], LIF [23,24] and MS [11,[25][26][27][28]. LC, HPLC, and UHPLC have also been applied for the determination of amino acids [9,17,[29][30][31][32][33][34][35]. GC has been used to analyze amino acids both with a flame ionization detector [36,37] and with MS.…”

Section: Introductionmentioning

confidence: 99%

Derivatization coupled to headspace programmed‐temperature vaporizer gas chromatography with mass spectrometry for the determination of amino acids: Application to urine samples

Paredes

Pinto

Pavón

et al. 2016

J of Separation Science

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A new method based on headspace programmed-temperature vaporizer gas chromatography with mass spectrometry has been developed and validated for the determination of amino acids (alanine, sarcosine, ethylglycine, valine, leucine, and proline) in human urine samples. Derivatization with ethyl chloroformate was employed successfully to determine the amino acids. The derivatization reaction conditions as well as the variables of the headspace sampling were optimized. The existence of a matrix effect was checked and the analytical characteristics of the method were determined. The limits of detection were 0.15-2.89 mg/L, and the limits of quantification were 0.46-8.67 mg/L. The instrumental repeatability was 1.6-11.5%. The quantification of the amino acids in six urine samples from healthy subjects was performed with the method developed with the one-point standard additions protocol, with norleucine as the internal standard.

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Validation of an analytical method for human plasma free amino acids by high-performance liquid chromatography ionization mass spectrometry using automated precolumn derivatization

Cited by 55 publications

References 29 publications

Amino Acid Analysis Using a Cartridge-Type Monolithic Silica Column

Amino Acid Analysis Using a Cartridge-Type Monolithic Silica Column

Association between plasma α‐aminobutyric acid and depressive symptoms in older community‐dwelling adults in Japan

Derivatization coupled to headspace programmed‐temperature vaporizer gas chromatography with mass spectrometry for the determination of amino acids: Application to urine samples

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