Validation of an Automated Immunoturbidimetric Assay for Fibrinogen/Fibrin Degradation Products Measurement and its Correlation to a Semi-Quantitative Latex Agglutination Test

Martinuzzo, Marta; Ujhelly, Cecilia; Barrera, Luis; Adamo, Maria A D; López, Marina Sol; Otaso, Juan Carlos; Oyhamburu, José

doi:10.7754/clin.lab.2016.160223

Cited by 2 publications

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“…The LATIA system was developed based on the antigen-antibody immune response, and has been utilized for determining clinical values of CRP [19], fibrinogen [20], and Helicobacter pylori antibodies [21] in laboratories and hospitals. The system measures the aggregation of antigens and latex-linked antibodies.…”

Section: Discussionmentioning

confidence: 99%

The novel latex agglutination turbidimetric immunoassay system for simultaneous measurements of calprotectin and hemoglobin in feces

et al. 2019

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Background/Aims Fecal calprotectin (Fcal) as well as the fecal immunochemical test (FIT) are useful biomarkers for detecting activity and mucosal healing in inflammatory bowel diseases. Here, we report the performance of simultaneous measurements of Fcal and FIT for ulcerative colitis (UC) patients using the newly-developed latex agglutination turbidimetric immunoassay (LATIA) system. Methods Fcal and hemoglobin were measured by the LATIA system in 152 UC patients who underwent colonoscopy. Fcal was also quantified with a conventional enzyme-linked immunosorbent assay (ELISA). Fecal markers were evaluated in conjunction with the mucosal status of UC, which was assessed via the Mayo endoscopic subscore (MES) classification. Results The LATIA system could quantify calprotectin and hemoglobin simultaneously with the same fecal samples within 10 minutes. The values of the Fcal-LATIA closely correlated with those of the Fcal-ELISA (Spearman rank correlation coefficient, r=0.84; P <0.0001). The values of Fcal for each assay and the FIT all significantly correlated with the MESs (Spearman rank correlation coefficient, Fcal-LATIA: r=0.58, Fcal-ELISA: r=0.55, and FIT: r=0.72). The mucosal healing predictability (determined by an MES of 0 alone) of the Fcal-LATIA, Fcal-ELISA, and FIT-LATIA with the cutoffs determined by receiver operating characteristic curve analysis was 0.79, 0.78, and 0.92 for sensitivity, respectively, and 0.78, 0.69, and 0.73 for specificity, respectively. Conclusions The performance of the novel Fcal-LATIA was equivalent to that of the conventional Fcal assay. Simultaneous measurements with FITs would promote the clinical relevance of fecal biomarkers in UC.

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Section: Discussionmentioning

confidence: 99%

The novel latex agglutination turbidimetric immunoassay system for simultaneous measurements of calprotectin and hemoglobin in feces

et al. 2019

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“…Fibrin is also an early and sensitive indicator of hypercoagulative states with increased brinogen-brin turnover, which is associated with the pathogenesis and diagnosis of pathogenesis of intravascular coagulation (DIC) (1,2,3,4,5) and thrombosis. [3][4][5][6] Up to now, a number of methods to determine brin have been reported, including the latex agglutination assay, 7,8 enzyme linked immunosorbent assay (ELISA), [9][10][11] and radioimmunoassay (RIA). 12 The latex agglutination assay is the most used method because it is very sensitive and precise.…”

Section: Introductionmentioning

confidence: 99%

Sensitive fluorescent detection of fibrin based on the inner filter effect of gold nanoparticles

et al. 2017

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Validation of an Automated Immunoturbidimetric Assay for Fibrinogen/Fibrin Degradation Products Measurement and its Correlation to a Semi-Quantitative Latex Agglutination Test

Abstract: AIMT presented good analytical performance, and the concordance with SLAT by comparison of patients' samples results was also good. The implementation of IMMT in the clinical laboratory is suitable and reliable.

Cited by 2 publications

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The novel latex agglutination turbidimetric immunoassay system for simultaneous measurements of calprotectin and hemoglobin in feces

The novel latex agglutination turbidimetric immunoassay system for simultaneous measurements of calprotectin and hemoglobin in feces

Sensitive fluorescent detection of fibrin based on the inner filter effect of gold nanoparticles

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