Validation of an LC-MS/MS Method for the Quantification of Caffeine and Theobromine Using Non-Matched Matrix Calibration Curve

Mendes, Vera M.; Coelho, Margarida; Tomé, Ângelo R.; Cunha, Rodrigo A.; Manadas, Bruno

doi:10.3390/molecules24162863

Cited by 26 publications

(20 citation statements)

References 41 publications

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“…This fragmentation pattern is in accordance with that of Carnitine (Sowell et al, 2011). Peak 15 was identified as caffeine from its MS 2 fragments (Mendes et al, 2019).…”

Section: Nitrogenous Compoundssupporting

confidence: 81%

Non‐polar metabolites of green beans ( Phaseolus vulgaris L.) potentiate the antidiabetic activity of mesenchymal stem cells in streptozotocin‐induced diabetes in rats

Ezzat

Rahman

Salama

et al. 2022

Journal of Food Biochemistry

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Green beans (Phaseolus vulgaris L.) are consumed as pods or mature seeds (common beans). The pods were extracted with 95% ethanol and processed to prepare nonpolar and polar fractions. Comparing the antihyperglycemic activity of both fractions, non-polar fraction (NPF, 200 mg kg −1 day −1 ) lowered blood glucose in streptozotocin diabetic rats by 65% compared to 57% for the polar fraction at the same dose. When NPF treatment was combined with injection of mesenchymal stem cells (MSC) a 4.4fold increase in serum insulin and a 73.6% reduction in blood glucose were observed compared to untreated control. Additionally, a significant decrease in malondialdehyde (76.2%), nitric oxide (68.2%), cholesterol (76.1%), and triglycerides (69.5%) and a 1.75-fold increase in HDL concentrations were observed in the group treated with this combination compared to diabetic animals. Interestingly, NPF increased homing of MSC in pancreas potentiating their antidiabetic activity. Finally, 26 compounds were identified in NPF using LC/MS analysis and four were isolated in pure form. The isolated compounds namely calotroproceryl acetate, fridelin, calotroproceryl A, and stigmasterol showed good inhibitory activity against pancreatic lipase with IC 50 at 1.93, 1.07, 1.34 and 1.44-1 μg/ml, respectively. Additionally, these compounds inhibited α-amylase, albeit at higher concentration, with IC 50 at 248, 212, 254, and 155 μg/ ml for calotroproceryl acetate, fridelin, calotroproceryl A, and stigmasterol, respectively. Our results suggest that green beans extract can potentiate effect of MSC in diabetes directly due to its own antidiabetic effect and indirectly by increasing MSC homing in pancreatic tissues. Practical applicationsIt has been suggested in this study that green beans can improve hyperglycemia, oxidative balance in diabetes, so green beans can be promoted as a healthy nutrient for diabetic patients. Green beans also can enhance homing and differentiation of mesnchymal stem cells in the pancreas for future stem cell therapy of type I diabetes.

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“…This fragmentation pattern is in accordance with that of Carnitine (Sowell et al, 2011). Peak 15 was identified as caffeine from its MS 2 fragments (Mendes et al, 2019).…”

Section: Nitrogenous Compoundssupporting

confidence: 81%

Non‐polar metabolites of green beans ( Phaseolus vulgaris L.) potentiate the antidiabetic activity of mesenchymal stem cells in streptozotocin‐induced diabetes in rats

Ezzat

Rahman

Salama

et al. 2022

Journal of Food Biochemistry

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“…Parasitoids are likely at risk of toxicity transfer from their host, as shown, for example, for the snowdrop lectin entomotoxin transferred from the diet of M. persicae to tissues of Aphidius ervi (Hymenoptera: Braconidae) endoparasitoid pupae (Couty et al, 2001). The next steps of the work will be to perform LC-MS or HPLC analyses (e.g., Kim et al, 2011;Mendes et al, 2019) to try detecting if caffeineor its main metaboliteshas been transferred from aphid to parasitoid tissues. More generally, host quality (size, age, nutritional content, immune defences) is crucial for proper parasitoid development and survival (Harvey, 2000), but can be altered by the plant quality (Harvey and Gols, 2018).…”

Section: Discussionmentioning

confidence: 99%

Cascading effects of caffeine intake by primary consumers to the upper trophic level

Tougeron

Hance

2021

Bull. Entomol. Res.

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Secondary metabolites are central to understanding the evolution of plant–animal interactions. Direct effects on phytophagous animals are well-known, but how secondary consumers adjust their behavioural and physiological responses to the herbivore's diet remains more scarcely explored for some metabolites. Caffeine is a neuroactive compound that affects both the behaviour and physiology of several animal species, from humans to insects. It is an alkaloid present in nectar, leaves and even sap of numerous species of plants where it plays a role in chemical defences against herbivores and pathogens. Caffeine effects have been overlooked in generalist herbivores that are not specialized in coffee or tea plants. Using a host–parasitoid system, we show that caffeine intake at a relatively low dose affects longevity and fecundity of the primary consumer, but also indirectly of the secondary one, suggesting that this alkaloid and/or its effects can be transmitted through trophic levels and persist in the food chain. Parasitism success was lowered by ≈16% on hosts fed with caffeine, and parasitoids of the next generation that have developed in hosts fed on caffeine showed a reduced longevity, but no differences in mass and size were found. This study helps at better understanding how plant secondary metabolites, such as caffeine involved in plant–animal interactions, could affect primary consumers, could have knock-on effects on upper trophic levels over generations, and could modify interspecific interactions in multitrophic systems.

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“…Caffeine analysis was performed using Shimadzu 8040 LC-MS/MS (Shimadzu, Kyoto, Japan) equipped with an LC pump (LC-30AD-1 and LC-30AD-2), an autosampler (SIL 30AC), and a column oven (CTO-20AC). The analytical method was modified from Ref [ 39 ]. The system was interfaced through electrospray ionization (ESI) in the positive mode.…”

Section: Methodsmentioning

confidence: 99%

Effect of Application Amounts on In Vitro Dermal Absorption Test Using Caffeine and Testosterone

Kim

Lee

et al. 2021

Pharmaceutics

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Dermal absorption of chemicals is a key factor in risk assessment. This study investigated the effects of different amounts of application on dermal absorption and suggested an appropriate application dose for proper dermal absorption. Caffeine and testosterone were chosen as test compounds. An in vitro dermal absorption test was performed using a Franz diffusion cell. Different amounts (5, 10, 25, and 50 mg (or µL)/cm2) of semisolid (cream) and liquid (solution) formulations containing 1% caffeine and 0.1% testosterone were applied to rat and minipig (Micropig®) skins. After 24 h, the concentrations of both compounds remaining on the skin surface and in the stratum corneum, dermis and epidermis, and receptor fluid were determined using LC-MS / MS or HPLC. Dermal absorption of both compounds decreased with increasing amounts of application in both skin types (rat and minipig) and formulations (cream and solution). Especially, dermal absorptions (%) of both compounds at 50 mg (or µL)/cm2 was significantly lower compared to 5 or 10 mg (or µL)/cm2 in both rat and minipig skins. Therefore, a low dose (5 or 10 mg (or µL)/cm2) of the formulation should be applied to obtain conservative dermal absorption.

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Validation of an LC-MS/MS Method for the Quantification of Caffeine and Theobromine Using Non-Matched Matrix Calibration Curve

Cited by 26 publications

References 41 publications

Non‐polar metabolites of green beans ( Phaseolus vulgaris L.) potentiate the antidiabetic activity of mesenchymal stem cells in streptozotocin‐induced diabetes in rats

Non‐polar metabolites of green beans ( Phaseolus vulgaris L.) potentiate the antidiabetic activity of mesenchymal stem cells in streptozotocin‐induced diabetes in rats

Cascading effects of caffeine intake by primary consumers to the upper trophic level

Effect of Application Amounts on In Vitro Dermal Absorption Test Using Caffeine and Testosterone

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