Validation of Antidiabetic and Antihyperlipidemic Effects of 80% Methanolic Extract of the Lonchocarpus laxiflorus Leaves in Streptozotocin-Induced Diabetic Swiss Albino Mice

Abstract

Introduction. Diabetes mellitus (DM) is a chronic endocrine disorder that requires long-term treatment. In Ethiopian traditional medicine practice, plants have been provided with a vital role in fighting human and animal diseases since ancient times. The aqueous extract of Lonchocarpus laxiflorus (L. laxiflorus) leaves has been consumed for treating diabetes mellitus without confirming its safety and efficacy scientifically. This experiment aimed to evaluate the safety and antidiabetic efficacy of the leaf extract of L. laxiflorus in mice models. Methods. The crude extraction was conducted using a cold maceration technique and 80% methanol solvent. Normoglycemic, oral glucose-loaded, and streptozotocin-induced (STZ) diabetic models were employed. Male Swiss albino mice were randomly grouped into five categories( with six mice per group during normoglycemic, oral glucose-loadingtest as a negative control, positive control, and three treatment groups. In STZ-induced diabetic models, the groups include normal and diabetic negative control, diabetic positive control, and three diabetic treatment groups. The negative control groups received vehicles, the positive control received 5 mg/kg glibenclamide, and the treatment groups received the crude extract at 100, 200, and 400 mg/kg doses, respectively. Results. Up to 2000 mg/kg crude extract, neither signs of toxicity nor death were observed. In normoglycemic mice, there was a significant blood glucose reduction at 200 and 400 mg/kg doses starting from the 2nd h post-administration. The oral glucose load showed a significant antihyperglycemic effect at 200 and 400 mg/kg of the crude extract and glibenclamide. In STZ-induced diabetic models, the 200, 400 mg/kg crude extract, and glibenclamide showed a significant antidiabetic activity and enhancement of a good serum lipid profile. Conclusion. This study confirmed that the leaf of L. laxiflorus was safe and possesses antidiabetic and antidyslipidemic activities.

Antidiabetic potential of Hibiscus sabdariffa extract in alloxan-induced diabetic rats

“…Several studies have demonstrated the involvement of phytochemical compounds such as triterpenes [31,32], flavonoids [33][34][35], and tannins [36] for the management of diabetes. These compounds might act by several mechanisms such as enhancing insulin secretion [37] or regenerating insulinproducing pancreatic β-cell population in the islets of Langerhans [38][39][40]. Thus, H. sabdariffa extract may enhanced insulin secretion by stimulating the β-cell of the Langerhans islets of the pancreas as its also consists of flavonoids, phenols, and tannins.…”

Antioxidant and anti-inflammatory effects of Boswellia dalzielii and Hibiscus sabdariffa extracts in alloxan-induced diabetic rats

“…Our previous studies demonstrated that the hydroethanolic extracts of B. dalzielii and H. sabdariffa possessed hypoglycemic and hypolipidemic properties in alloxan-induced diabetic rats [ 13 , 14 ], notably through reducing blood glucose, improving glucose tolerance, and normalizing lipid profiles. These plants and their phytochemical compounds might act by several mechanisms, such as enhancing insulin secretion by stimulating the β-cell of the Langerhans islets or regenerating the insulin-producing pancreatic β-cell population in the islets of Langerhans [ 27 ]. We evaluated the antioxidant and anti-inflammatory properties of B. dalzielii and H. sabdariffa in alloxan-induced diabetic rats.…”