Validation of Antidiabetic Potential of Gymnocarpos decandrus Forssk

Hypouricemic effect of gallic acid, a bioactive compound fromSonneratia apetalaleaves and branches, on hyperuricemic mice

“…39 Besides, vitexin was isolated from the ethanol extract of G. decandrus after chromatographic purification. 40 Based on these studies, we applied ethyl acetate and 60% alcohol to determine the anti-HUA fraction of SAL in this study. As shown in Fig.…”

HPLC-PDA-ESI-MS/MS Profiling and Anti-Biofilm Potential of Eucalyptussideroxylon Flowers

“…A ThermoFinnigan HPLC system using a Discovery HS F5 column (15 cm × 4.6 mm ID, 5 µm particles, Sigma-Aldrich Co Steinheim, Germany) was used. Water and acetonitrile (ACN) (Sigma-Aldrich GmbH, Berlin, Germany) (0.1% formic acid each) were used as a mobile phase adopting the same method reported in [26,27]. The autosampler surveyor ThermoQuest was used to inject the sample and Xcalibur software (Xcalibur TM 2.0.7, Thermo Fischer Scientific, Waltham, MA, USA) was used to control the system.…”

“…The autosampler surveyor ThermoQuest was used to inject the sample and Xcalibur software (Xcalibur TM 2.0.7, Thermo Fischer Scientific, Waltham, MA, USA) was used to control the system. The MS operated in the negative mode [26,27].…”

“…The fragment produced at m/z 249 in macrocarpals corresponds to the isopentyl diformyl phloroglucinol moiety that resulted from the typical cleavage of the sesquiterpene moiety. Oleuropeic acid derivatives: Seven oleuropeic acid derivatives were tentatively identified (22)(23)(24)(25)(26)(27)(28). All of them are oleuropeic acid-containing carbohydrates (oleuropeic acid glucosides); i.e., carbohydrate monoterpene esters.…”

“…Furthermore, fragment ions at m/z 255, 271 and 285 were detected corresponding to [M-H-CO-H] − , resulting from fragmentation of the kaempferol, quercetin and isorhamnetin aglycones, respectively [47]. A total of 16 flavonoids were tentatively identified, of which eleven were O-glycosides, one was C-glycoside (37) and four were flavonoid aglycones (41)(42)(43)(44); this in addition to eucalmaidin D, quercetin-4'-O-(6-O-oleuropeoyl)-R-D-glucopyranoside (26), which was listed under the abovementioned oleuropeic acid derivatives class. Eucalmaidin D (26) is a rare example of an α-configured glucoside found in nature.…”