Validation of chimerism in pediatric recipients of allogeneic hematopoietic stem cell transplantation (HSCT) a comparison between two methods

Kletzel, Morris; Huang, Wei; Olszewski, Marie; Khan, Sana

doi:10.4161/chim.23158

Cited by 27 publications

(28 citation statements)

References 24 publications

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“…As the minimum detection level for this technique is 0.01%, 22,23 we define complete chimerism (CC) as a recipient-cell percentage inferior to 0.01%, and MC as a recipient-cell percentage superior to this threshold. IMC was defined as an increase of the host cell percentage by at least 0.1% (that is, the detection of at least 0.1% recipient cells when previously strictly inferior to 0.1% or any increase of at least 0.1% above the 0.1% threshold).…”

Section: Definitionsmentioning

confidence: 99%

“…Quantitative PCR analysis using insertion/deletion polymorphism (indel qrtPCR) is a well-characterized and simple technique, which can be performed in peripheral blood with a better sensitivity compared with standard STR-PCR (0.01-0.1% vs 1-5%). 22,23 While this technique has been previously used by others, [24][25][26] its usefulness to predict relapse at the recipient's cell level o1% has never been clearly evaluated.…”

Section: Introductionmentioning

confidence: 99%

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Chimerism analysis in peripheral blood using indel quantitative real-time PCR is a useful tool to predict post-transplant relapse in acute leukemia

Jacque

Nguyen

Golmard

et al. 2014

Bone Marrow Transplant

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Detection of increasing mixed chimerism (IMC) using standard PCR correlates with relapse after allo-SCT for acute leukemias (ALs). Quantitative real-time PCR of insertion/deletion polymorphism (indel qrtPCR) is a much more sensitive method, which can be performed on peripheral blood. We studied the significance of low increases of recipient cells (0.1%) detected by indel qrtPCR in a cohort of 89 transplants. We did not observe relapse among the 32 patients with no IMC. Fifty-seven patients presented a first IMC, which was followed by four different scenarios: a decreasing MC (26 cases, no relapse), a stable MC (1 case, 1 relapse), a second IMC (24 cases, 15 relapse) or no control of chimerism (6 cases, 5 relapses). In multivariate analysis, detection of two successive IMCs was strongly associated with relapse (hazard ratio (HR): 9.4, 95% confidence interval (CI): 3.8-23; Po0.0001). Among the 57 patients who presented at least one IMC, 27 underwent immunomodulation (tapering of immunosuppression or donor lymphocyte injection), leading to a 1-year relapse rate of 15.7% vs 57.6% in the 30 other patients (P = 0.0007). Altogether, these results indicate that chimerism analysis using indel qrtPCR in peripheral blood is a useful tool for detection of relapse in patients transplanted for AL.

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Section: Definitionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chimerism analysis in peripheral blood using indel quantitative real-time PCR is a useful tool to predict post-transplant relapse in acute leukemia

Jacque

Nguyen

Golmard

et al. 2014

Bone Marrow Transplant

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“…However, other methods, such as quantitative real-time PCR, may be preferable when a very small minor component is to be detected. 13,14 In this study, we have described the use of a set of Indel polymorphic loci to determine post-transplant chimerism. To our knowledge, this is the first published report using Indel analysis for Post-Transplant Chimerism Detection Using 38 Indel Locithis purpose.…”

Section: Discussionmentioning

confidence: 99%

Analysis of post-transplant chimerism by using a single amplification reaction of 38 Indel polymorphic loci

Santurtún

Riancho

Yáñez

et al. 2014

Bone Marrow Transplant

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In order to detect chimerism after allogeneic hematopoietic SCT (HSCT), several methods have been developed. In this study we describe the use of a set of insertion/deletion (Indel) polymorphic loci to determine the level of donor cell engraftment. We analyzed 50 DNA samples from patients who had undergone HSCT, and also several artificial chimeric samples created by mixing different DNA specimens from non-transplanted donors in various proportions. A specific set of 38 autosomic Indel polymorphisms were analyzed. For comparison purposes, a set of 15 short tandem repeats (STRs) were analyzed using the Identifiler Plus Amplification Kit. Our results suggest that Indel-based and STR-based procedures behave similarly in most cases. However, Indel analysis may provide additional information in some cases with a small minor chimeric component or when the presence of stutter bands complicates chimerism estimation.

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“…126 Multicopy genes localized on the Y-chromosome represent suitable targets for reproducible and sensitive analyses by qPCR. 29,57,117,127,128 Its application is, however, restricted to sex-mismatched combinations, whereby male DNA targets are targeted in a female host.…”

Section: Pcr-based Methodologiesmentioning

confidence: 99%

Naturally acquired microchimerism

et al. 2014

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Validation of chimerism in pediatric recipients of allogeneic hematopoietic stem cell transplantation (HSCT) a comparison between two methods

Cited by 27 publications

References 24 publications

Chimerism analysis in peripheral blood using indel quantitative real-time PCR is a useful tool to predict post-transplant relapse in acute leukemia

Chimerism analysis in peripheral blood using indel quantitative real-time PCR is a useful tool to predict post-transplant relapse in acute leukemia

Analysis of post-transplant chimerism by using a single amplification reaction of 38 Indel polymorphic loci

Naturally acquired microchimerism

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