Validation of determination of plasma metabolites derived from thyme bioactive compounds by improved liquid chromatography coupled to tandem mass spectrometry

Rubió, Laura; Serra, Aida; Macià, Alba; Borràs, Xenia; Romero, Marı́a-Paz; Motilva, Marı́a-José

doi:10.1016/j.jchromb.2012.07.040

Cited by 35 publications

(33 citation statements)

References 27 publications

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“…In this study, besides the parent thymol itself (major compound), six metabolites were identified: 2,5‐dihydroxy‐ p ‐cymene, 2‐(2‐hydroxy‐4‐methyIphenyl)propan‐1‐ol, 5‐hydroxymethyl‐2‐(1‐methylethyl)phenol, 2‐(4‐hydroxymethyl‐2‐hydroxyphenyl)propan‐1‐ol, 2‐(2‐hydroxy‐4‐methyIphenyl)propionic acid, and 3‐hydroxy‐4‐(1‐methylethyl)benzoic acid. In rat plasma, thymol sulfate and thymol glucuronide have also been detected after the oral administration of a hydroethanolic thyme extract (1.5 g; Rubió et al, ). Thymol is also excreted in the urine as sulfate and glucuronide conjugates in rabbits and dogs (Williams, ).…”

Section: Resultsmentioning

confidence: 99%

“…propan-1-ol, 2-(2-hydroxy-4-methyIphenyl)propionic acid, and 3hydroxy-4-(1-methylethyl)benzoic acid. In rat plasma, thymol sulfate and thymol glucuronide have also been detected after the oral administration of a hydroethanolic thyme extract (1.5 g; Rubió et al, 2012). Thymol is also excreted in the urine as sulfate and glucuronide conjugates in rabbits and dogs (Williams, 1959).…”

Section: Bioavailability Metabolism and Distributionmentioning

confidence: 99%

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Thymol, thyme, and other plant sources: Health and potential uses

Salehi

Mishra

Shukla

et al. 2018

Phytotherapy Research

396

243

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Thymol is a naturally occurring phenol monoterpene derivative of cymene and isomer of carvacrol. Thymol (10-64%) is one of the major constituent of essential oils of thyme (Thymus vulgaris L., Lamiaceae), a medicinal plant with several therapeutic properties. This plant, native to Mediterranean regions, is commonly used as a culinary herb and also with a long history of use for different medicinal purposes. Nowadays, thymol and thyme present a wide range of functional possibilities in pharmacy, food, and cosmetic industry. The interest in the formulation of pharmaceuticals, nutraceuticals, and cosmeceuticals based on thymol is due to several studies that have evaluated the potential therapeutic uses of this compound for the treatment of disorders affecting the respiratory, nervous, and cardiovascular systems. Moreover, this compound also exhibits antimicrobial, antioxidant, anticarcinogenesis, anti-inflammatory, and antispasmodic activities, as well as a potential as a growth enhancer and immunomodulator. In the present review, these bioactivities have been covered because some of them can contribute to explain the ethnopharmacology of thymol and its main source, T. vulgaris. Other important aspects about thymol are discussed: its toxicity and bioavailability, metabolism, and distribution in animals and humans.

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Section: Resultsmentioning

confidence: 99%

Section: Bioavailability Metabolism and Distributionmentioning

confidence: 99%

Thymol, thyme, and other plant sources: Health and potential uses

Salehi

Mishra

Shukla

et al. 2018

Phytotherapy Research

396

243

View full text Add to dashboard Cite

show abstract

“…Carotenoids, retinol, ubiquinol, tocopherols, and phenolic compounds from olive oil and thyme were analyzed by high‐pressure liquid chromatography (HPLC) . Compounds were identified by their retention time compared with pure standards or, when unavailable (lutein and β‐cryptoxanthin), with compounds obtained and purified in the laboratory.…”

Section: Methodsmentioning

confidence: 99%

Determinants of HDL Cholesterol Efflux Capacity after Virgin Olive Oil Ingestion: Interrelationships with Fluidity of HDL Monolayer

Fernández‐Castillejo¹,

Rubió²,

Hernáez

et al. 2017

Molecular Nutrition Food Res

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Scope Cholesterol efflux capacity of HDL (CEC) is inversely associated with cardiovascular risk. HDL composition, fluidity, oxidation, and size are related with CEC. We aimed to assess which HDL parameters were CEC determinants after virgin olive oil (VOO) ingestion. Methods and Results Post-hoc analyses from the VOHF study, a crossover intervention with three types of VOO. We assessed the relationship of 3-week changes in HDL-related variables after intervention periods with independence of the type of VOO. After univariate analyses, mixed linear models were fitted with variables related with CEC and fluidity. Fluidity and Apolipoprotein (Apo)A-I content in HDL was directly associated, and HDL oxidative status inversely, with CEC. A reduction in free cholesterol, an increase in triglycerides in HDL, and a decrease in small HDL particle number or an increase in HDL mean size, were associated to HDL fluidity. Conclusions HDL fluidity, ApoA-I concentration, and oxidative status are major determinants for CEC after VOO. The impact on CEC of changes in free cholesterol and triglycerides in HDL, and those of small HDL or HDL mean size, could be mechanistically linked through HDL fluidity. Our work points out novel therapeutic targets to improve HDL functionality in humans through nutritional or pharmacological interventions.

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“…Suárez et al () suggested an LC–MS method for identifying phenolic compounds, their sulfate and glucuronide metabolites in human plasma after oral administration of olive oil. Consequently, this method was applied and validated for the determination of thymol sulfate in plasma using microelution solid‐phase extraction (SPE) plates, where thymol sulfate was isolated as a standard using semi‐preparative HPLC from rat plasma after the ingestion of the thyme extract (Rubió et al, ). As mentioned above, the described methods for determination of thymol metabolites in plasma and urine have been reported in the literature so far.…”

Section: Introductionmentioning

confidence: 99%

“…of thymol sulfate in plasma using microelution solid-phase extraction (SPE) plates, where thymol sulfate was isolated as a standard using semi-preparative HPLC from rat plasma after the ingestion of the thyme extract (Rubió et al, 2012). As mentioned above, the described methods for determination of thymol metabolites in plasma and urine have been reported in the literature so far.…”

mentioning

confidence: 99%

Identification and quantification of thymol metabolites in plasma, liver and duodenal wall of broiler chickens using UHPLC‐ESI‐QTOF‐MS

Pisarčíková

Ocelova

Faix

et al. 2016

Biomedical Chromatography

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In the present study, we aimed to develop a method for thymol sulfate and thymol glucuronide determination in plasma, liver and duodenal wall of broiler chickens after feeding with a Thymus vulgaris essential oil at the different concentrations (0.01, 0.05 and 0.1% w/w). UHPLC coupled with accurate-mass QTOF-MS was used for identification and quantification of thymol metabolites. Novel Waters Oasis Prime HLB solid-phase extraction cartridges were applied to sample clean-up with extraction recoveries ranged from 85 to 92%. The presence of thymol glucuronide was confirmed by MS software according to molecular formula, score, mass error and double bond equivalent. In terms of validation, calibration curves of thymol sulfate were constructed in matrix samples with linearity from 3.91 to 250.0 ng/mL and correlation coefficients were within the range of 0.9979-0.9995. Limits of detection were 0.97, 0.29 and 0.63 ng/mL and limits of quantification were 3.23, 0.97 and 2.09 ng/mL for plasma, liver and duodenal wall, respectively. Intra-day and inter-day precision expressed as relative standard deviation were <4.35%. To highlight, thymol metabolites were directly detected for the first time in liver and duodenal wall and this method was shown to be successfully applicable for investigation of thymol metabolism in chickens after thyme essential oil ingestion.

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Validation of determination of plasma metabolites derived from thyme bioactive compounds by improved liquid chromatography coupled to tandem mass spectrometry

Cited by 35 publications

References 27 publications

Thymol, thyme, and other plant sources: Health and potential uses

Thymol, thyme, and other plant sources: Health and potential uses

Determinants of HDL Cholesterol Efflux Capacity after Virgin Olive Oil Ingestion: Interrelationships with Fluidity of HDL Monolayer

Identification and quantification of thymol metabolites in plasma, liver and duodenal wall of broiler chickens using UHPLC‐ESI‐QTOF‐MS

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