Validation of ELISAs for the detection of antibodies to Sarcoptes scabiei in pigs

Heijden, H.M.J.F. van der; Rambags, P.G.M.; Elbers, A.R.W.; Maanen, C. van; Hunneman, W. A.

doi:10.1016/s0304-4017(00)00197-7

Cited by 32 publications

(27 citation statements)

References 14 publications

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“…Similarly, Kircher and Zimmermann (1999) examined serum samples from piglets from an infected herd (regular treatment of sows with organophosphates) using the CHEKIT-ELISA and also found that all animals were positive in the first week of life. In contrast, Van der Heijden et al (2000) investigated colostral antibody titres in an infected herd by use of AHS and CHEKITELISAs, but detected only few serologically positive animals (AHS-ELISA: two positive, CHEKIT-ELISA: one positive, one doubtful, both not identical to those positive in the AHS-ELISA).…”

Section: Discussionmentioning

confidence: 88%

“…The ELISA-2001 was more sensitive from day 63 when 28-56% more positive animals were detected. Neither Van der Heijden et al (2000) nor Bornstein and Zakrisson (1993) detected active antibody responses by means of the CHEKIT or the Uppsala-ELISA which use S. scabiei var. vulpis from fox as the antigen.…”

Section: Discussionmentioning

confidence: 98%

“…The design of the study does not allow us to say whether there are any false positive results, but all of the sera were from a mangy farm and there were also no serological differences between the skin scraping positive and negative animals ( Table 2). Van der Heijden et al (2000) compared the CHEKIT-ELISA with their AHS-ELISA during a Sarcoptes contact infection, and the CHEKIT-ELISA detected the first positive animals on day 84, 6 weeks after the AHS-ELISA. In addition, the seroprevalence detected by the CHEKIT-ELISA was much lower than the AHS-ELISA.…”

Section: Discussionmentioning

confidence: 99%

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On the substantial variation in serological responses in pigs to Sarcoptes scabiei var. suis using different commercially available indirect enzyme-linked immunosorbent assays

2004

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Two trials were carried out to compare the use of different enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies against Sarcoptes scabiei var. suis. In the first trial, we employed four tests and 70 selected sera from a closed pig farm with sarcoptic mange. The sera were taken from the breeding area as well as from the finishing unit and compared with skin scrapings. The SARCOPTES-ELISA 2001 yielded the most positive results (88.58%), followed by the ELISA of the National Veterinary Institute, Uppsala (70%), the Acar-Test P-ELISA (52.86%), skin scrapings (48.57%), and the CHEKIT Sarcoptest (30%). In the second trial, eight litters from infected sows were examined from weeks 1-12 of life using the CHEKIT Sarcoptest and the SARCOPTES-ELISA 2001. The presence of maternal antibodies was highest on day 7 in both ELISAs and could be detected until weeks 5-9 of life. Antibodies increased as a result of an active immune response between days 56 and 63 in the SARCOPTES-ELISA 2001 and between days 70 and 77 of life in the CHEKIT Sarcoptest. Significant differences between the first and second litters were observed.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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On the substantial variation in serological responses in pigs to Sarcoptes scabiei var. suis using different commercially available indirect enzyme-linked immunosorbent assays

2004

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“…When skin scrapings were taken as gold standard, the test showed higher sensitivity (100 %) with lower specificity (32.8 %). Van der Heijden et al (2000) validated an ELISA for detection of antibodies against S. scabiei in pigs. Antibody detection ELISA has been used successfully to monitor the eradication strategies against sarcoptic mange infestation in swine herds (Jacobson et al 1999).…”

Section: Diagnosismentioning

confidence: 99%

Sarcoptic mange infestation in pigs: an overview

Laha

2014

J Parasit Dis

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Sarcoptic mange infestation in pigs is caused by Sarcoptes scabiei var. suis. It is the most common mange infestation of pigs. The parasite is distributed worldwide. Pig owners are generally concerned about the internal parasitic infections and ignored the external parasitic infestations. But the external parasitic infestation with S. scabiei var. suis has economic significance as it causes morbidity, mortality, decreased fertility and feed conversion ratio in pigs. Keeping in view of importance of S. scabies var. suis infestation in pigs, this communication discussed about the present and past research works done on S. scabies var. suis infestation in pigs, particularly its prevalence, life cycle, pathological lesions, clinical symptoms, haematobiochemical changes, diagnosis, treatment and control, to have an idea about this infestation at a glance. It has been concluded that the research work done on sarcoptic mange infestation in pigs in India is less in comparison to other countries. It may be due to its consideration as a neglected parasite or due to it's under report. Organization of awareness programs for the farmers by extension personalities or other authorities might be able to save the farmers from economic losses due to this infestation.

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“…It has been reported that more than 50% of skin scrapes can be negative in canine scabies infections [24]. In recent years, many enzyme-linked immunosorbent assays (ELISA), based on crude mite extracts have been developed for several host species [6,7,13,31,36]. S. scabiei is the source of multiple antigenic molecules [1][2][3] and the varieties of S. scabiei from different animals introduce both cross-reactive and variety specific antigens into the host [5,13].…”

Section: Introductionmentioning

confidence: 99%

Identification and heterologous expression of aSarcoptes scabieicDNA encoding a structural antigen with immunodiagnostic potential

et al. 2007

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-The mite Sarcoptes scabiei causes sarcoptic mange (or scabies), a disease of considerable human and veterinary significance. An S. scabiei cDNA clone of about 2 kb was isolated from a S. scabiei var. hominis expression library by immunological screening using blood serum from a naturally infected chamois (Rupicapra rupicapra). The nucleotide sequence of the identified cDNA contains an open reading frame of 1930 bp that encodes a 642 amino acid polypeptide. This polypeptide shows tandem repeats of a glycine-serine rich 20 residue sequence followed by a unique C-terminal glutamate rich 54 residue sequence. The cDNA or the deduced polypeptide did not show significant similarities to any of the sequences in the databases. A carboxyl-terminal fragment of this polypeptide (residues 380 to 642) was efficiently expressed in Escherichia coli as a fusion with Glutathione S-transferase and then was used to produce a specific antiserum. The antigen encoded by the cDNA was located at the integument of the mite's epidermis and the cavities surrounding its vital organs. Western blot analysis of mite extracts using the specific antiserum against the recombinant protein identified antigens larger that 60 kDa indicating that the isolated cDNA did not contain the full ORF. Moreover, we designed a diagnostic assay based on the carboxyl-terminal fragment of the antigen for the identification of infected animals.Sarcoptes scabiei / cDNA library / immunolocalisation / sarcoptic mange diagnosis / ELISA

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Validation of ELISAs for the detection of antibodies to Sarcoptes scabiei in pigs

Cited by 32 publications

References 14 publications

On the substantial variation in serological responses in pigs to Sarcoptes scabiei var. suis using different commercially available indirect enzyme-linked immunosorbent assays

On the substantial variation in serological responses in pigs to Sarcoptes scabiei var. suis using different commercially available indirect enzyme-linked immunosorbent assays

Sarcoptic mange infestation in pigs: an overview

Identification and heterologous expression of aSarcoptes scabieicDNA encoding a structural antigen with immunodiagnostic potential

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