Validation of expression stability of reference genes in response to herbicide stress in wild oat (Avena ludoviciana)

Akbarabadi, Ali; Ismaili, Ahmad; Kahrizi, Danial; Firouzabadi, Farhad Nazarian

doi:10.14715/cmb/2018.64.4.19

Cited by 28 publications

(25 citation statements)

References 39 publications

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“…There are several reports of gene expression and ways to control it (19)(20)(21)(22)(23)(24)(25). Numerous genetic and non-genetic factors affect gene expression.…”

Section: Discussionmentioning

confidence: 99%

Effects of ethyl acetate extract of peony (Paeonia suffruticosa) seed coat on the proliferation and apoptosis of oral squamous carcinoma cells through miR-424-3p/STAT3/Survivin pathway

Ren

2020

Cell Mol Biol (Noisy-le-grand)

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Oral squamous cell carcinoma is one of the most common high malignant tumors. This experiment aimed to investigate whether ethyl acetate extract of peony (Paeonia suffruticosa) seed coat could affect the proliferation and apoptosis of oral squamous carcinoma cells by regulating the miR-424-3p/STAT3/ Survivin pathway. For this purpose, oral squamous cell carcinoma cell CAL27 was cultured in vitro, and cells were treated with ethyl acetate extract of peony seed coat at different concentrations. MTT was used to detect cell proliferation. Flow cytometry was used to detect apoptosis. qRT-PCR was used to detect the expression level of miR-424-3p. The miR-424-3p mimics and anti-miR-424-3p were transfected into CAL27 cells respectively, and the cell proliferation and apoptosis were detected by the above method. Western blot method was used to detect the expression of PCNA, Bcl-2, Bax, p-STAT3 and Survivin protein. Results showed that ethyl acetate extract of peony seed coat could reduce cell proliferation rate and the protein levels of PCNA, Bcl-2, p-STAT3, Survivin and the expression level of miR-424-3p (P<0.05), increase apoptosis rate and the protein level of Bax (P<0.05). After transfection with anti-miR-424-3p, the cell proliferation rate, the protein levels of PCNA and Bcl-2 were significantly reduced (P<0.05), the apoptosis rate and the protein level of Bax were significantly increased (P<0.05), while the effect of miR-424-3p mimics was the opposite. Transfection of miR-424-3p mimics could significantly reduce the regulatory effect of ethyl acetate extract of peony seed coat on CAL27 cell proliferation, apoptosis and STAT3/Survivin pathway. It concluded that ethyl acetate extract of peony seed coat could inhibit the activation of the STAT3/Survivin signaling pathway by down-regulating the expression of miR-424-3p, thereby inhibiting the proliferation of oral squamous carcinoma cells and inducing apoptosis.

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“…There are several reports of gene expression and ways to control it (19)(20)(21)(22)(23)(24)(25). Numerous genetic and non-genetic factors affect gene expression.…”

Section: Discussionmentioning

confidence: 99%

Effects of ethyl acetate extract of peony (Paeonia suffruticosa) seed coat on the proliferation and apoptosis of oral squamous carcinoma cells through miR-424-3p/STAT3/Survivin pathway

Ren

2020

Cell Mol Biol (Noisy-le-grand)

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“…8 , Avena sp. 30,31,32 , Descurainia sp. 33 etc., but till date, there are no reports on the systematic selection of stable reference genes under any conditions for barren brome or any other related brome species.…”

Section: Discussionmentioning

confidence: 99%

Identification of the Most Suitable Reference Gene for Gene Expression Studies With Development and Abiotic Stress Response in Bromus Sterilis

Sen

Hamouzová

Košnarová

et al. 2021

Preprint

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Bromus sterilis is an annual weedy grass, causing high yield losses in winter cereals. Frequent use of herbicides had led to the evolution of herbicide-resistance in this species. Mechanisms underlying herbicide resistance in B. sterilis must be uncovered because this problem is becoming a global threat. qRT-PCR and the next-generation sequencing technologies can contribute to elucidation of the resistance mechanisms. Although qRT-PCR can calculate precise fold changes, its preciseness depends on the expression of reference genes. Regardless of stable expression in any given condition, no gene can act as a universal reference gene. Hence, it is necessary to identify the suitable reference gene for each species. To our knowledge, there are no reports on the suitable reference gene in any brome species so far. Thus, in this paper, the stability of eight genes were evaluated using qRT-PCR experiments followed by expression stability ranking via five most commonly used softwares for reference gene selection. Our findings suggest using a combination of 18S rRNA and ACCase to normalise the qRT-PCR data in B. sterilis. Besides, reference genes are also recommended for different experimental conditions. The present study outcomes will facilitate future molecular work in B. sterilis and other related grass species.

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“…In genetics, gene expression is one of the most important and essential issues that assistance a genotype to appear as a phenotype. The genetic code stored in the DNA strands is interpreted by gene expression, and the characteristics and expression of the gene will cause a phenotype in the organism (22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33). In this research, we investigated on the MiR-381-3p/RAB2A axis activates cell proliferation and inhibits cell apoptosis in bladder cancer.…”

Section: Discussionmentioning

confidence: 99%

MiR-381-3p/RAB2A axis activates cell proliferation and inhibits cell apoptosis in bladder cancer

Sun

Hu²,

Wang

et al. 2020

Cell Mol Biol (Noisy-le-grand)

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Acting as a really common cancer in the world, bladder cancer has taken many people's life away. MiRNAs and mRNA have been reported can regulate the expression of cancers. In this study, the role of RAB2A and miR-381-3p was fully studied in bladder cancer. qRT-PCR assay probe the expression of RAB2A and miR-381-3p in bladder cancer cells. Meanwhile, colony formation assay, EdU assay, flow cytometry analysis, JC-1 assay and western blot assay were implemented to detect the progression of bladder cancer cells. Silenced RAB2A could reduce the cell proliferation of bladder cancer, and activate the apoptosis.Meanwhile, miR-381-3p could bind to RAB2A in bladder cancer cells and overexpressed miR-381-3p could inhibit the progression of bladder cancer cells. MiR-381-3p/RAB2A axis activates cell proliferation and inhibits cell apoptosis in bladder cancer.

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Validation of expression stability of reference genes in response to herbicide stress in wild oat (Avena ludoviciana)

Cited by 28 publications

References 39 publications

Effects of ethyl acetate extract of peony (Paeonia suffruticosa) seed coat on the proliferation and apoptosis of oral squamous carcinoma cells through miR-424-3p/STAT3/Survivin pathway

Effects of ethyl acetate extract of peony (Paeonia suffruticosa) seed coat on the proliferation and apoptosis of oral squamous carcinoma cells through miR-424-3p/STAT3/Survivin pathway

Identification of the Most Suitable Reference Gene for Gene Expression Studies With Development and Abiotic Stress Response in Bromus Sterilis

MiR-381-3p/RAB2A axis activates cell proliferation and inhibits cell apoptosis in bladder cancer

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