Validation of Fusarium Head Blight Resistance QTL in US Winter Wheat

Abstract

Fusarium head blight (FHB), primarily caused by Fusarium graminearum Schwabe [telemorph: Gibberella zeae Schw. (Petch)], can significantly reduce the grain quality of wheat (Triticum aestivum L.) due to mycotoxin contamination. Two US soft red winter wheat cultivars, Bess and NC‐Neuse, have moderate resistance to FHB. The objective of this study was to validate genomic regions associated with FHB resistance identified in previous studies involving NC‐Neuse and the cultivar Truman, a full‐sib of Bess. A total of 98 doubled haploid lines derived from the cross Bess × NC‐Neuse were evaluated in inoculated, mist‐irrigated field nurseries. The lines were evaluated for FHB incidence, severity, Fusarium‐damaged kernels, and deoxynivalenol content in seven environments between 2011 and 2014. A 3338‐cM linkage map was developed based on 4014 simple sequence repeat and single nucleotide polymorphism markers. Twelve quantitative trait loci (QTL) associated with FHB resistance were identified. NC‐Neuse alleles provided resistance at QTL on five chromosomes and Bess alleles provided resistance at QTL on five other chromosomes. Alignment of linkage maps revealed that five of these QTL were overlapping with previously identified regions. Quantitative trait loci on chromosomes 1A, 4A, and 6A identified in this study overlapped with QTL regions identified in NC‐Neuse, and QTL identified on chromosomes 2B and 3B overlapped with QTL regions identified in Truman. A preliminary test using Kompetitive Allele‐Specific polymerase chain reaction assays on recent Uniform Southern Winter Wheat Scab Nursery entries showed that the assays developed for Qfhb.nc‐2B.1 may be good candidates for use in marker‐assisted selection.

Genetic Analysis of Fusarium Head Blight Resistance in CIMMYT Bread Wheat Line C615 Using Traditional and Conditional QTL Mapping

“…The QTL on chromosome 2AL found after spray inoculation was linked to SNP marker BS00022896_51 and explained 5.23–9.34% of the phenotypic variation. Three QTL clusters for FHB resistance were previously detected on chromosomes 2AS (2) and 2AL, and these were closely linked to SSR markers Xbarc124, Xgwm122 , and Xgwm311 , respectively ( Gervais et al, 2003 ; Ma et al, 2006 ; Holzapfel et al, 2008 ; Lu et al, 2013 ; Zhang et al, 2014 ; Giancaspro et al, 2016 ; Petersen et al, 2016 , 2017 ). Markers Xgwm311 (at 772 Mb) and BS00022896_51 (at 612 Mb), separated by a physical distance of 160 Mb, are in different deletion bins ( Qi et al, 2004 ).…”

Identification and validation of Fusarium head blight resistance QTL in the U.S. soft red winter wheat cultivar ‘Jamestown’

“…Previously, mapping studies have identified QTL for FHB resistance on chromosome 1B from European sources (Arina, Biscay, Cansas, Fundulea 201R, History, Lynx, Remus, Rialto, Romanus, Pirat, and Sincron), Asian sources (CM82036, Kitahonami, and Wangshuibai), and one variety (Seri82) from the International Maize and Wheat Improvement Center (CIMMYT). Studies have also been conducted on mapping sources of resistance in U.S. wheat varieties, including varieties: Bess (QTL on 2B and 3B; McKendry, Tague, Wright, & Tremain, 2007), Ernie (QTL on 2B, 3B, and 5A; Liu et al., 2007), Massey (QTL on 1A, 1D, 3B, and 4D; Liu et al., 2013), Goldfield (QTL on B and 7B; Gilsinger, Kong, Shen, & Ohm, 2005), Neuse (QTL on 1A, 4A, and 6A; Petersen et al., 2017), Truman (QTL on 2B and 3B; Islam et al., 2016). However, none of the soft winter wheat sources of FHB resistance mapped so far are assigned to chromosome 1B.…”

Validation of Grain Yield QTLs from Soft Winter Wheat Using a CIMMYT Spring Wheat Panel

“…This is particularly true of QTLs for grain yield (GY), as it is a quantitative trait influenced by many loci with mostly small effects, making the identification and validation of significant marker‐trait associations a challenge. Although improvement of GY has remained the top priority of wheat ( Triticum aestivum L.) breeding programs (Green et al, 2012), validation and utilization of GY QTL slows the progress seen in more qualitatively inherited traits such as resistance to biotic stresses (Bokore et al, 2017; Petersen et al, 2017; Prat et al, 2017).…”