Validation of gene expression profiles of candidate genes using low density array in peripheral blood of tobacco consuming head and neck cancer patients and auto/taxi drivers with preneoplastic lesions

Hasan, Feza; Yadav, Vinay Kumar; Katiyar, Tridiv; Yadav, Sanjay; Pandey, Rahul; Mehrotra, Divya; Hadi, Rahat; Singh, Sudhir; Bhatt, M L B; Parmar, Devendra

doi:10.1016/j.ygeno.2019.04.003

Cited by 6 publications

(4 citation statements)

References 41 publications

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“…Infection of mice with H.pylori leads to premalignant lesions in the gastric mucosa that have EMT phenotypes [ 145 ]. EMT gene expression is elevated in blood samples from tobacco exposed patients with premalignant head and neck squamous cell carcinoma lesions [ 146 ]. In premalignant cervical lesions, EMT facilitates progression from low to high grade lesions [ 147 ].…”

Section: Frizzleds In Cancer Emtmentioning

confidence: 99%

Cancer chemoprevention through Frizzled receptors and EMT

et al. 2021

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Frizzled (FZD) transmembrane receptors are well known for their role in β-catenin signaling and development and now understanding of their role in the context of cancer is growing. FZDs are often associated with the process of epithelial to mesenchymal transition (EMT) through β-catenin, but some also influence EMT through non-canonical pathways. With ten different FZDs, there is a wide range of activity from oncogenic to tumor suppressive depending on the tissue context. Alterations in FZD signaling can occur during development of premalignant lesions, supporting their potential as targets of chemoprevention agents. Agonizing or antagonizing FZD activity may affect EMT, which is a key process in lesion progression often targeted by chemoprevention agents. Recent studies identified a specific FZD as important for activity of an EMT inhibiting chemopreventive agent and other studies have highlighted the previously unrecognized potential for targeting small molecules to FZD receptors. This work demonstrates the value of investigating FZDs in chemoprevention and here we provide a review of FZDs in cancer EMT and their potential as chemoprevention targets.

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Section: Frizzleds In Cancer Emtmentioning

confidence: 99%

Cancer chemoprevention through Frizzled receptors and EMT

et al. 2021

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“…Hospital-based pharmacokinetic studies have also revealed significantly lower elimination of tobacco specific carcinogens in urine samples of lung cancer patients with CYP2A6*4C (Fujieda et al, 2004;Malaiyandi et al, 2006;Park et al, 2017;Yuan et al, 2017). In a recent study, Hasan et al (2020) from our laboratory has shown a decrease in the copy number of CYP2A6 in peripheral blood and biopsy samples obtained from HNSCC cases with variant genotypes of CYP2A6 genotypes (Hasan et al, 2019(Hasan et al, , 2020. However, as compared to deletion polymorphism, another variant of CYP2A6 (CYP2A6*1B), resulting from CYP2A7 gene conversion in the 3 0 -untranslated region, has been shown to increase the stability of mRNA, though a very modest difference has been reported in the clearance of nicotine when compared with CYP2A6*1A/*1A, the common genotype (Mwenifumbo et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Polymorphism in cytochrome P4502A6 reduces the risk to head and neck cancer and modifies the treatment outcome

Yadav

Katiyar

Ruwali

et al. 2021

Environ and Mol Mutagen

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The present case-control study consisting of 1300 cases of head and neck squamous cell carcinoma (HNSCC) and the equal number of controls aimed to investigate the association of functionally important polymorphisms in cytochrome P4502A6 (CYP2A6*1B, CYP2A6*4C, CYP2A6*9-rs28399433) with HNSCC and the treatment response in cases receiving a combination of chemotherapy/radiotherapy (CT/RT). A significant decrease in risk to HNSCC was observed in the cases with deletion (CYP2A6*4B and CYP2A6*4C) or reduced activity genotypes (CYP2A6*9) of CYP2A6. This risk to HNSCC was further reduced significantly in tobacco users among the cases when compared to nontobacco users among the cases. The risk was also reduced to a slightly greater extent in alcohol users among the cases when compared to nonalcohol users among the cases. In contrast with decreased risk to HNSCC, almost half of the cases with variant genotypes of CYP2A6 (CYP2A6*1A/*4C+*1B/*4C+*4C/*4C and *9/*9) did not respond to the treatment. Likewise, the survival rate in cases receiving the treatment, after 55 months of follow-up was significantly lower in cases with deletion (6.3%) or reduced activity (11.9%) allele than in the cases with common alleles (41%). The present study has shown that CYP2A6 polymorphism significantly reduces the risk to HNSCC. Our data further suggested that CYP2A6 polymorphism may worsen the treatment outcome in the cases receiving CT/RT.

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“…We selected the 96 target SNPs, based on the SNPs of tobacco smoking-responsive genes, of which expression levels were altered by tobacco smoking in our previous microarray study [15], i.e., ACTG1, DEFA4, VAV3, FCGR3A, etc., as well as the SNPs related to metabolism, e.g., CYP2A6, CYP1B1, CYP2E1, and NQO1, addiction [38], e.g., CHRNA5/A3/B4, 5-HTTLPR, and DRD2 [39], risks for lung cancer, e.g., CYP1A1, GSTP1, and MPO1 [39,40], DNA repair, e.g., ERCC1, MGMT, XRCC1, etc. [41], and epigenetic modulation, e.g., HDAC1 and MTHFR [33], of tobacco smoking (supplement Table 1).…”

Section: Targeted Genotypingmentioning

confidence: 99%

Tobacco Smoking as an EDC in Aging

Lee,

Na,

Chang

et al. 2024

Preprint

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Tobacco smoke is an environmental mixture including polycyclic aromatic hydrocarbons and may interfere in endocrine system as an EDC in aging. Thus, we performed a molecular epidemiological approach with in-depth biological monitoring of combustible cigarette smoking among adolescents and adults in South Korea (N=620) with exposure biomarkers, i.e., exhaled CO, urinary cotinine, t,t-muconic acid (TTMA), malondialdehyde (MDA), and obtained information of their lifestyle and tobacco addiction status. We also diagnosed the genetic polymorphisms on the 96 SNPs for tobacco-metabolism, -addiction, and expression differences and compared mtDNA abnormalities in buccal and blood cells. As results, there were positive associations among the above tobacco exposure biomarkers. Man or youth smokers showed high frequency in some of mtDNA alteration, such as ‘SNPs for inconsistent bases between buccal and blood cells’. Among the SNPs, the polymorphisms on SULT1A1, DRD2, and ADH1B were related to multi-exposure biomarkers. Interestingly, youth showed similar levels of urinary TTMA and MDA to adults, although their pack-year was approx. 1/6 volume of that of adults. We also observed the negative association between urinary MDA levels and the growth rate in the adolescents (p<0.05). In conclusion, the present biological monitoring provides high susceptible population, i.e., adolescents rather than adults, and reliable genetic factors affecting tobacco exposure. The inferred environment-gene-gene interaction suggests tobacco smoking accelerats aging in adolescents as an EDC.

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Validation of gene expression profiles of candidate genes using low density array in peripheral blood of tobacco consuming head and neck cancer patients and auto/taxi drivers with preneoplastic lesions

Cited by 6 publications

References 41 publications

Cancer chemoprevention through Frizzled receptors and EMT

Cancer chemoprevention through Frizzled receptors and EMT

Polymorphism in cytochrome P4502A6 reduces the risk to head and neck cancer and modifies the treatment outcome

Tobacco Smoking as an EDC in Aging

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