Validation of high-performance liquid chromatographic assay methods for the analysis of carboplatin in plasma ultrafiltrate

Determination of carboplatin in human plasma using HybridSPE-precipitation along with liquid chromatography–tandem mass spectrometry

“…With regard to selective methods for the determination of free carboplatin, high performance liquid chromatography (HPLC) separation coupled to various detection techniques appears to be the method of choice. Carboplatin has been analyzed using reversed phase [9,10], hydrophilic [1], and strong cation exchange [4] chromatographic conditions. Due to its very polar property, carboplatin has minimal k values on most reversed phase columns.…”

“…Due to its very polar property, carboplatin has minimal k values on most reversed phase columns. Different detection techniques including UV [9,11], electrochemical [12], MS [10,13], ICP-MS [14,15], MS/MS [4] have been applied in the determination of carboplatin. UV detection of carboplatin is limited by its low sensitivity and non-specificity because of the lack of favorable UV absorption properties and the interference of either endogenous compounds or biotransformation products.…”

“…UV detection of carboplatin is limited by its low sensitivity and non-specificity because of the lack of favorable UV absorption properties and the interference of either endogenous compounds or biotransformation products. UV detection usually requires HPLC run times of over 10 min and detection limits of over 50.0 ng/mL [9]. Electrochemical detection of carboplatin also has very limited applicability due to reproducibility issues and reliance on HPLC separation of carboplatin from possible interferences [12].…”

A high-performance liquid chromatography–tandem mass spectrometry method for the clinical combination study of carboplatin and anti-tumor agent eribulin mesylate (E7389) in human plasma

“…Detection of intact carboplatin is usually required for pharmacokinetic investigations. High-performance liquid chromatography (HPLC) appears to be the method of choice for carboplatin determination in biological samples, with assays using either normal-phase [2][3][4][5] or reverse-phase liquid chromatography (LC) [6][7][8][9][10][11][12][13][14][15][16][17] with UV [2,3,[8][9][10][11]14,15], electrochemical detection [6], LC/mass spectrometry (LC/MS) [12,16] or LC-inductively coupled plasma-MS (LC-ICP-MS) [13,17]. UV detection is affected by non-specificity due to the lack of absorbance by carboplatin and the interference of either endogenous compounds or biotransformation products.…”

HPLC method for the determination of carboplatin and paclitaxel with cremophorEL in an amphiphilic polymer matrix

“…It possesses broad antineoplastic activity. Development of a method by RP-HPLC was difficult owing to its extreme hydrophilicity; it has minimal k values on most ODS columns and is hence not retained on RP-based cartridges and it does not get partitioned extensively into water immiscible solvents [6,7]. Reported methods of analysis of platinum analogs are based upon atomic absorption (flame and nonflame) [8], inductively coupled plasma atomic emission (ICP-AE) [9], electroanalytical techniques [10], neutron activation analysis [11] and gas [12] and normal phase liquid chromatography [13].…”