Validation of High Pressure Resin Impregnation Technique for High Resolution Confocal Imaging of Geological Samples

Hassan, Ahmed; Yutkin, Maxim; Chandra, Viswasanthi; Patzek, Tadeusz W

doi:10.2118/195020-ms

Cited by 2 publications

(1 citation statement)

References 45 publications

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“…In LSCM imaging, the vertical depth of the optical section is in excess of 30 μm, and it can even reach ∼150 μm in some quartz sandstones. A bunch of geological materials such as sandstone, carbonates, − mudstones (shales), marble, and even many engineering materials such as polymer membrane, concretes, , and catalysts have been visualized by LSCM. Additionally, many geological applications, including fluid transport in geological materials and brittle failure processes as well as stress-induced damage in rocks, have been studied based on the LSCM imaging data of the pore architecture.…”

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confidence: 99%

Background-Free Deep Fluorescent Imaging of a Pore Architecture in Geomaterials Based on Magnetic Upconversion Nanoprobes

Chen

Kang

et al. 2022

ACS Earth Space Chem.

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Fluorescent microscopy, one of the most widespread means for visualizing the pore system in geomaterials, always suffers from the random interference of autofluorescence of minerals and other rock matrices and, more importantly, is limited to a certain depth for 3D imaging. Here, two-photon excitation fluorescence microscopy, a novel nonlinear optical mode, is unlocked for imaging of the pore architecture in geomaterials with no background and significantly increased imaging depth by the use of a rare-earth-doped upconversion nanoparticle (NaYF 4 :Yb, Er) as a two-photon excitation probe. The superparamagnetic Fe 3 O 4 nanoparticle is integrated with the NaYF 4 :Yb, Er nanoparticle to make them easily drive into pores under the control of an external magnetic field. The rare-earth-doped upconversion nanoparticle offers a unique anti-Stokes optical property, which can be easily distinguished from autofluorescence (Stokes fluorescence), eliminating background fluorescence from the geomaterial matrix for three typical sediment rocks, such as sandstone, carbonate, and shale. Encouragingly, two-photon excitation fluorescence microscopy catches the upconversion fluorescence emission signal at the depth of 450 μm for a randomly selected sandstone using serial optical sectioning without destroying the rock matrix. Coupled with the rare-earth-doped upconversion nanoparticle as a pore probe, two-photon excitation fluorescence microscopy, using near-infrared light as the incident light instead of UV or visible light, with resulting less sensitivity to adsorption and scattering, as well as hosting the spatial confinement of signal generation with nonlinear excitation, provides a new opportunity for the deep imaging of pore architectures in geological materials.

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confidence: 99%

Background-Free Deep Fluorescent Imaging of a Pore Architecture in Geomaterials Based on Magnetic Upconversion Nanoprobes

Chen

Kang

et al. 2022

ACS Earth Space Chem.

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3D Confocal Imaging Methodology Optimized for Pore Space Characterization of Microporous Carbonate Reservoirs

Hassan

Chandra

Yutkin

et al. 2020

Day 2 Tue, January 14, 2020

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Microporous carbonates host a significant portion of the remaining oil-in-place in the giant carbonate reservoirs of the Middle East. Carbonates host wide range of pore sizes, however the key element influencing hydrocarbon flow is pore interconnectivity. We evaluate the use of confocal microscopy to image fluorescent epoxy pore casts of microporous carbonates. The acquired high-resolution 3D confocal images are used to gain invaluable insights on the interconnectivity between macroporosity and microporosity. We analyzed the sensitivity of quality of epoxy pore cast images to: fluorochrome selection, objective lens, and imaging medium by imaging standard fluorescent spherical beads. Guided by the sensitivity results, we acquired 3D images of the multi-modal pore space in an Indiana limestone sample with lateral- and axial-resolution of 0.36 µm and 2 µm, respectively. And we were able to identify the multi-scale pore types in the studied carbonate sample and highlight their interconnectivity.

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Validation of High Pressure Resin Impregnation Technique for High Resolution Confocal Imaging of Geological Samples

Cited by 2 publications

References 45 publications

Background-Free Deep Fluorescent Imaging of a Pore Architecture in Geomaterials Based on Magnetic Upconversion Nanoprobes

Background-Free Deep Fluorescent Imaging of a Pore Architecture in Geomaterials Based on Magnetic Upconversion Nanoprobes

3D Confocal Imaging Methodology Optimized for Pore Space Characterization of Microporous Carbonate Reservoirs

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