Validation of HPLC method for determination of tetracycline residues in chicken meat and liver

Shalaby, Al Shimaa Gamal; Salama, N. A.; Abou-Raya, S.H.; Emam, Wafaa H.; Mehaya, Fathy M.

doi:10.1016/j.foodchem.2010.07.048

Cited by 111 publications

(75 citation statements)

References 24 publications

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“…Because DC is one of the most frequently used antibiotic in chickens, it was necessary to develop a suitable method for determination of this compound in chicken tissues. Many studies using analytical methods for determination of DC in animal tissues have been presented (4,5,8,12,17,18,21,22). Most of them did not describe the application of methods in the analysis of DC in samples collected from treated animals.…”

Section: Introductionmentioning

confidence: 99%

LC-MS/MS analysis of doxycycline residues in chicken tissues after oral administration

Gajda¹,

Posyniak²,

Tomczyk

2014

Bulletin of the Veterinary Institute in Pulawy

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For the purpose of quantitative determination of doxycycline (DC) residues in tissues, a sensitive liquid chromatographytandem mass spectrometry (LC-MS/MS) method was developed. The method was used to determine DC residues in chicken tissues (breast and thigh muscle, liver and kidney) after oral administration with drinking water to five-weak-old broiler chickens. The DC was administered for five consecutive days at a therapeutic dose of 10 mg/kg b.w. once a day. The tissues were collected after 6 h, 24 h, 7 d, and 8 d. The method was validated and the decision limit was established for muscle -109.2 µg/kg, for liver -326.1 µg/kg, and for kidney -634.0 µg/kg. The detection limit was 2 µg/kg and the limit of quantification was 5 µg/kg. In a short period after ceasing the treatment, the detected concentrations of DC were much higher than the established maximum residue limit values. The highest residue concentrations of DC were observed in the kidney, followed by the liver and muscle. The lowest concentration of DC was determined in tight muscle.

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Section: Introductionmentioning

confidence: 99%

LC-MS/MS analysis of doxycycline residues in chicken tissues after oral administration

Gajda¹,

Posyniak²,

Tomczyk

2014

Bulletin of the Veterinary Institute in Pulawy

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“…Triethylamine is a very common additive used to block silanols groups in reversed-phase chromatography when separating basic analytes (21). Oxalic acid in a mobile phase shows a significant efficiency in softening the effect of residual silanols on the stationary phase and good TCs separation without tailing (6,16,24,30). However, it should be taken into account that mobile phases containing non-volatile compounds used in LC -MS or LC -MS/MS may cause clogging at the interface and a build-up of deposits in the ion source (26,27).…”

Section: Discussionmentioning

confidence: 99%

“…For effective extraction of TCs from tissues with high extraction efficiency, buffers within the range of 4.0 -5.0 pH values are recommended (20). In most studies, extraction with a mild acidic solvent containing EDTA (0.1 M EDTA -McIlvaine buffer, pH 4.0), citric acid, and oxalic acid has been described to overcome these undesirable properties with acceptable extraction efficiencies (7,15,24,27,28,30). Cooper et al found the citrate buffer (pH 5.0) with ethyl acetate to be the most effective in TCs isolation (13).…”

Section: Discussionmentioning

confidence: 99%

Liquid chromatography – tandem mass spectrometry method for the determination of ten tetracycline residues in muscle samples

Gajda

Posyniak

2015

Bulletin of the Veterinary Institute in Pulawy

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A liquid chromatography -tandem mass spectrometry (LC-MS/MS) method for the determination of oxytetracycline (OTC), 4-epi oxytetracycline (4-epi OTC), tetracycline (TC), 4-epi tetracycline (4-epi TC), chlortetracycline (CTC), 4-epi chlortetracycline (4-epi CTC), doxycycline (DC), minocycline (MINO), methacycline (META) and rolitetracycline (ROLI) residues in muscles was developed. The procedure consisted of an oxalic acid extraction followed by protein removal with trichloroacetic acid. Further solid phase clean-up on polymeric (Strata X) reversed phase columns was performed to obtain an extract suitable for LC-MS/MS analysis. The tetracyclines were separated on a C 18 analytical column with mobile phase consisting of 0.01% formic acid in acetonitrile and 0.01% formic acid in water in gradient mode. The method was validated according to the Commission Decision 2002/657/EC. The recoveries of all target compounds were 91.8% -103.6%. The decision limits were from 109.0 to 119.8 µg/kg and detection capability varied within the range of 122.2 to 137.6 µg/kg, depending on the analyte.

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“…Most of them use HPLC with ultraviolet (UV) [14,15] or fluorescence [16] detection. Fluorescence has the advantage of being more sensitive and selective, but does not have the same range of applicability as UV making UV the most widely used detection method [17].…”

Section: Methods Validationmentioning

confidence: 99%

Development of an Improved HPLC-PDA Method for the Determination of Tolfenamic Acid in Meat and Milk

L¹,

HC²,

ML³

et al. 2017

J Chromatogr Sep Tech

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Validation of HPLC method for determination of tetracycline residues in chicken meat and liver

Cited by 111 publications

References 24 publications

LC-MS/MS analysis of doxycycline residues in chicken tissues after oral administration

LC-MS/MS analysis of doxycycline residues in chicken tissues after oral administration

Liquid chromatography – tandem mass spectrometry method for the determination of ten tetracycline residues in muscle samples

Development of an Improved HPLC-PDA Method for the Determination of Tolfenamic Acid in Meat and Milk

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