Validation of LC for the Determination of  -Mangostin in Mangosteen Peel Extract: A Tool for Quality Assessment of Garcinia mangostana L.

Abstract

Mangosteen, Garcinia mangostana L., is known as the "Queen of fruits" and can be cultivated in the tropical rainforest such as Malaysia, Indonesia, and Thailand. Compounds isolated from the fruit peel of mangosteen contain abundant xanthones (especially alpha-mangostin). It has been used as traditional medicine such as anti-inflammatory and antibacterial and is popularly applied to cosmetic and pharmaceutical products. However, there is little information for quality and quantity determination of alpha-mangostin in mangosteen. Thus, the aim of this study was to set up a validated and stability-indicated isocratic reverse-phase high-performance liquid chromatographic (HPLC) method for quality control and quantity determination of a-mangostin from mangosteen peel extract. The assay was fully validated and shown to be linear (r(2) > 0.999), sensitive (LOD = 0.02 microg/mL and LOQ = 0.08 microg/mL), accurate (intra-day was between 98.1-100.8%, inter-day was between 90.0-101.3%), precise (intra-day variation < or = 1.8%, inter-day variation < or = 4.3%), specific, and with good recovery. Total analysis was approximately 8 min. The finalized method is also a stability-indicating assay. The present method should be useful for analytical research and for routine quality control analysis of alpha-mangostin in mangosteen peel extract and products of mangosteen.

Retrospective Validation of a Structure-Based Virtual Screening Protocol to Identify Ligands for Estrogen Receptor Alpha and Its Application to Identify the Alpha-Mangostin Binding Pose

“…2) resulted in 1000 selected poses from 1000 different iterations of the protocol. The compound α-mangostin, which can be found in the pericarp of Garcinia mangostana, L. [21,33] is a known ligand for ERα [21,33]. Garcinia mangostana, L. has recently gained its popularity [34] due to its applications as herbal medicines to treat inflammation and bacterial infections [33] as well as its application in cancer chemoprevention [35].…”

High Performance Liquid Chromatography (HPLC) Analysis, Antioxidant, Antiaggregation of Mangosteen Peel Extract (Garcinia mangostana L.)

“…MPE comprised of 75%-85% α-mangostin and 5%-15% γ-mangostin [30], [31]. MPE contain abundant xanthones (especially α-mangostin) [32], high yield of xanthones such as α-and γ-mangostin in the pericarp of G. mangostana [33]. .27 b A The data showed mean ± standard deviation.…”

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD VALIDATION OF α-MANGOSTIN ASSAY IN MANGOSTEEN (Garcinia mangostana L.) FRUIT RIND EXTRACT FORMULATED IN ORAL SOLUTION

“…Untuk menganalisis α-mangostin dalam sediaan tersebut dibutuhkan metode yang selektif dan sensitif seperti kromatografi cair kinerja tinggi (KCKT). KCKT telah banyak digunakan untuk menetapkan kadar α-mangostin di dalam ekstrak dari kulit buah manggis (Aisha et al, 2013;Yodhnu et al, 2009;Pothitirat and Gritsanapan, 2009;Islam and Begum, 2011;Jujun et al, 2009), ekstrak yang dienkapsulasi dalam PLGA (Poly Lactic-co-Glycolic Acid) microspheres (Ali et al, 2012), dan α-mangostin dalam plasma darah tikus (Syamsudin et al, 2010). Kondisi optimum KCKT untuk menetapkan kadar α-mangostin dalam larutan oral ekstrak kulit buah manggis dicapai menggunakan oktadesilsilan (C18) sebagai fase diam, metanol-air (90:10) sebagai fase gerak dengan laju alir 1 mL/menit dan detektor UV 316 nm (Nurhidayati et al, 2014).…”