2004
DOI: 10.1002/adic.200490117
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Validation of LC/MS Electrospray Ionisation Method for the Estimation of Ursodiol in Human Plasma and ITS Application in Bioequivalence STUDY

Abstract: A novel High Performance Liquid Chromatography-electrospray mass spectrometric method has been developed for the estimation of Ursodiol (Ursodeoxycholic acid)--a bile acid, in human plasma using Ornidazole as internal standard. The methodology involved solid phase extraction of the analyte from human plasma matrix. The chromatographic separation was achieved within seven minutes by an isocratic mobile phase containing 1.0 mM ammonium acetate and Acetonitrile (65:35, v/v), flowing through XTerra MS C18, 100 x 2… Show more

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Cited by 8 publications
(1 citation statement)
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“…Several analytical methods have been developed for determining bile acids in biological fluids, among them the UCDA and its metabolites, each one with its own particularities 3,8,13. The development of a suitable and simple method in LC-MS/MS (HPLC-tandem mass spectrometr y) for UDCA, GUDCA, and TUDCA determination could be useful in enabling an understanding of the original compound’s metabolism as well as in obtaining new generic drugs that are more accessible and less costly for the population and the pharmaceutical industry.…”
Section: Introductionmentioning
confidence: 99%
“…Several analytical methods have been developed for determining bile acids in biological fluids, among them the UCDA and its metabolites, each one with its own particularities 3,8,13. The development of a suitable and simple method in LC-MS/MS (HPLC-tandem mass spectrometr y) for UDCA, GUDCA, and TUDCA determination could be useful in enabling an understanding of the original compound’s metabolism as well as in obtaining new generic drugs that are more accessible and less costly for the population and the pharmaceutical industry.…”
Section: Introductionmentioning
confidence: 99%