Validation of microRNA expression profile in Oral Lichenoid Disease through cytological samples

Abstract

BackgroundTo validate oral exfoliative cytology in the analysis of the microRNA expression profile in Oral Lichenoid Disease (OLD).Material and MethodsThe expression of 13 microRNAs identified and presented by our group in a previous study was analyzed in 26 cases, 16 diagnosed as OLD and 10 controls with no oral mucosal pathology. Cytological samples from the oral mucosa obtained using an Orcellex toothbrush were analyzed using RT-qPCR and TaqMan microRNA assays.ResultsThe aberrant expression was validated for 2 microRNAs (miR-146a-5p and miR-7-1-3p) of those previously recognized in the biopsy study.ConclusionsThis is the first time that oral exfoliative cytology is validated in a study of the alterations of the expression of microRNAs in OLD. The alteration of miR-146a and miR-7 compared to controls was validated. These microRNAs are associated with both inflammatory and carcinogenic phenomena that are involved in the etiopathogenesis of this potentially malignant oral disorder. Key words:microRNAs, lichen planus, epigenetics, cytological techniques.

Approaches to the Issue of Screening for Diseases of the Oral Mucosa: A Present View

“…In lichen planus, cytological examination usually reveals a large number of lymphocytes in the affected area, many activated macrophages, and plasma cells are less common [25].…”

Optimized liquid‐based cytology for the cellular and molecular analysis of oral keratinocytes: A promising diagnostic tool

“…In addition, it reduces the problem related to sampling in the preparation of smears, it reduces false negative rates; and therefore, it improves the sensitivity and quality of the cytology 6,7 . However, the lack of studies reporting oral LBC‐standardized protocols, and the elevated cost of materials and methodologies, challenges its adoption in the daily clinical practice and research 5,8‐13 . There is a need of having a low cost, reproducible, and easily accessible protocol; available worldwide that could be implemented for the cellular and molecular study of several oral mucosa lesions, so that cytopathologists could have an additional and complementary diagnostic tool, with a considerable potential for their early detection.…”

Expression and implications of cytology‐based miRNAs in oral premalignant and malignant lesions

“…According to the search strategy described in Table S1, a total of 9 eligible studies on 19 aberrant miRNAs are identified (Cheng et al, 2021;Gissi et al, 2018;He et al, 2016;Lin et al, 2022;Setién-Olarra et al, 2019;Tu et al, 2022;Yap et al, 2018;Yap et al, 2019;Zhou et al, 2017). There are 243 OSCC patients, 116 OPMD patients, and 307 normal controls.…”