Validation of QTLs for seed weight in a backcross population derived from an interspecific cross in soybean [Glycine max (L.) Merr.]

Abstract

Soybean [Glycine max (L.) Merr.] is a major oilseed crop of India. Seed weight is an important yield component trait which should be suitably optimized in soybean varieties to maximize productivity. To validate 100-seed weightquantitave trait loci (QTL), a backcross population of soybean was developed from a cross between wild species Glycine soja (Sieb. and Zucc.) and Indian soybean cultivar JS 335. The BC2 backcross population was evaluated for three yield component traits, namely 100-seed weight, number of seeds/plant and seed yield/plant in BC2F2, BC2F3 and BC2F4 generation. Six QTLs reported to be associated with 100-seed weight in soybean were selected for QTL validation. SSR markers linked with two major QTLs for 100-seed weight could be validated successfully. One QTL, on linkage group D1a between Satt580-Satt179, identified for 100-seed weight explained 19.18% ofphenotypic variance for combined data of three years. The second QTL for 100-seed weight was identified on linkage group C2 between marker Sat_251 and Sat_238 which contributed 10.97 and 9.28% of phenotypic variance in year 2015 and 2016, respectively.

Development of improved genotypes for extra early maturity, higher yield and Mungbean Yellow Mosaic India Virus (MYMIV) resistance in soybean (Glycine max)

“…G. soja has been reported as a potential donor for early maturity, MYMIV resistance, grain yield, 100-seed weight and several other key traits (Kumawat et al . 2019 b ).…”

“…G. soja has been reported as a potential donor for early maturity, MYMIV resistance, grain yield, 100-seed weight and several other key traits (Kumawat et al 2019b). For conversion of JS 335 (a highly susceptible genotype for yellow mosaic disease) into its MYMIV resistant version, G. soja was crossed with JS 335, and through a series of backcrossing and phenotyping, the MYMIV resistant gene was introgressed.…”

“…Apart from yellow mosaic disease resistance, G. soja also possesses quantitative trait loci (QTLs) for yield and yield attributing traits like 100-seed weight and seed yield (Concibido et al . 2003; Kumawat et al . 2019 b ).…”

“…Considering losses caused by disease and availability of resistance sources, it is imperative to develop elite soybean varieties with durable resistance to yellow mosaic disease and high yield, either through molecular breeding or conventional plant breeding approaches. Apart from yellow mosaic disease resistance, G. soja also possesses quantitative trait loci (QTLs) for yield and yield attributing traits like 100-seed weight and seed yield (Concibido et al 2003;Kumawat et al 2019b).…”