Validation Of Radiation Sterilization Dose For Proteases Immobilized On Aldehyde-Containing Textile Carriers

Dyankova, S.; Doneva, M.; Tsvetkov, Tsvetan Dimitrov

doi:10.9790/3013-04010063068

Cited by 1 publication

(1 citation statement)

References 9 publications

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“…Gamma radiation, however, is reported to be a suitable sterilization method of biological/bioactive products or tissues, maintaining approximately 90% of protease activity when doses between 0 and 10 kGy are applied. Bioactivity of collagen type I is preserved after sterilization with a total dose of up to 20 kGy [Furuta et al, 2002;Wiegand et al, 2009;Dyankova et al, 2014]. The integrity of MMP-2 and MMP-9 and their proactive forms were maintained in bovine dentin substrate even after using a high dose of gamma radiation (25 kGy) [Gasque et al 2011].…”

Section: Discussionmentioning

confidence: 99%

Chlorhexidine, a Matrix Metalloproteinase Inhibitor and the Development of Secondary Caries Wall Lesions in a Microcosm Biofilm Model

Maske¹,

Kuper²,

Cenci

et al. 2018

Caries Res

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This study investigated the role of a matrix metalloproteinase (MMP) inhibitor (CHX 2%) in the development of secondary caries wall lesions in different interface conditions with small (run 1) and wider gaps (run 2). Dentin discs were restored and pretreated with or without CHX 2%. In run 1, interfaces were made with gaps of 30, 60, or 90 µm. Interfaces with composite placed directly onto the dentin were either bonded (Adper Single Bond 2) or not bonded. In run 2, interfaces were made with gaps of 100 µm, with or without adhesive on the composite side (CLEARFIL SE Bond). Interfaces were either bonded or not bonded, as in run 1. Microcosm biofilms were grown on dentin-composite samples for 14 days. Caries lesion outcomes were analyzed by transversal wavelength-independent microradiography at 3 locations: the outer surface, and the interface wall at a distance of 200 and 500 µm from the gap entrance. Linear regression analyses showed that pretreatment with MMP inhibitor did not influence progression of the wall lesion at any location (p ≥ 0.218). Interfaces with intentional gaps showed positive and significant effect on the wall lesion progression at 200 µm from the gap entrance (p ≤ 0.005). A small trend of increase in wall lesion development was observed at the 200-µm location when bonding was present on the composite side. In conclusion, the dentin pretreatment with CHX 2% was not able to slow down the development of secondary caries wall lesions in small and wide gaps in this biofilm model.

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Section: Discussionmentioning

confidence: 99%

Chlorhexidine, a Matrix Metalloproteinase Inhibitor and the Development of Secondary Caries Wall Lesions in a Microcosm Biofilm Model

Maske¹,

Kuper²,

Cenci

et al. 2018

Caries Res

View full text Add to dashboard Cite

show abstract

Validation Of Radiation Sterilization Dose For Proteases Immobilized On Aldehyde-Containing Textile Carriers

Abstract: ABSTRACT:The main objective of this work is to establish the parameters of radiation sterilization for three proteolytic enzymes (papain, trypsin and subtilisin)

Cited by 1 publication

References 9 publications

Chlorhexidine, a Matrix Metalloproteinase Inhibitor and the Development of Secondary Caries Wall Lesions in a Microcosm Biofilm Model

Chlorhexidine, a Matrix Metalloproteinase Inhibitor and the Development of Secondary Caries Wall Lesions in a Microcosm Biofilm Model

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