Validation of Sennae Folium specification grade classification based on UPLC-Q-TOF/MS spectrum-effect relationship

An, Qi; Wang, Lei; Ding, Xiaoying; Yajun, Shen; Hao, Shenghui; Li, Wenjie; Li, Heng-yang; Wang, Tao; Zhan, Zhenlin; Zheng, Yuguang; Guo, Long; Zhang, Dan

doi:10.1016/j.arabjc.2022.104223

Cited by 10 publications

(3 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The creation of the MN was based on the similarity of MS/MS spectra, as visually presented in Figure 4 . In the figure, the node size is indicative of the relative quantity of the respective compound, and each node is depicted as a pie chart [ 20 ]. This diagram emphasizes the primary cluster of flavonoids, which encompasses compounds such as quercetin (peak 37, m / z 303.05, [M + H] + ), isoquercetin (peak 40, m / z 465.1206, [M + H] + ), kaempferol (peak 45, m / z 287.0548, [M + H] + ), and others [ 21 , 22 ].…”

Section: Resultsmentioning

confidence: 99%

Exploring Antioxidant and α-Glucosidase Inhibitory Activities in Mulberry Leaves (Morus alba L.) across Growth Stages: A Comprehensive Metabolomic Analysis with Chemometrics

Li,

Hao,

et al. 2023

Molecules

Self Cite

View full text Add to dashboard Cite

Metabolic product accumulation exhibited variations among mulberry (Morus alba L.) leaves (MLs) at distinct growth stages, and this assessment was conducted using a combination of analytical techniques including high-performance liquid chromatography (HPLC), gas chromatography–mass spectrometry (GC-MS), and liquid chromatography–mass spectrometry (LC-MS). Multivariate analysis was applied to the data, and the findings were correlated with antioxidant activity and α-glucosidase inhibitory effects in vitro. Statistical analyses divided the 27 batches of MLs at different growth stages into three distinct groups. In vitro assays for antioxidant activity and α-glucosidase inhibition revealed that IC50 values were highest at the Y23 stage, which corresponds to the ‘Frost Descends’ solar term. In summary, the results of this study indicate that MLs at different growth stages throughout the year can be categorized into three primary growth stages using traditional Chinese solar terms as reference points, based on the observed variations in metabolite content.

show abstract

Section: Resultsmentioning

confidence: 99%

Exploring Antioxidant and α-Glucosidase Inhibitory Activities in Mulberry Leaves (Morus alba L.) across Growth Stages: A Comprehensive Metabolomic Analysis with Chemometrics

Li,

Hao,

et al. 2023

Molecules

Self Cite

View full text Add to dashboard Cite

show abstract

“…The contribution of variables to the model can be explored through the variable importance in projection (VIP) value. The larger the VIP value (generally more than 1.0), the greater the contribution [58,59]. By screening the VIP values of different compounds, it is easy to find active compounds with high correlation coefficients with pharmacological indexes.…”

Section: Methods To Clarify the Contribution Of Each Component To Eff...mentioning

confidence: 99%

“…They found that the first premium grade was a green leaf, followed by a yellow leaf and diseased leaf. They identified that six sennosides might be the material bases for in vitro antibacterial activity and a marker for grade differentiation [58]. Similarly, this pattern has also been successfully applied to studying the spectrum-effect relationships of Notoginseng [62] of twelve different commodity specifications and Notopterygii Rhizoma [63] of three commercial specifications.…”

Section: Quality Evaluation Of Tcmmentioning

confidence: 99%

Spectrum–Effect Relationships as an Effective Approach for Quality Control of Natural Products: A Review

He,

Zhang,

Yang

et al. 2023

Molecules

View full text Add to dashboard Cite

As natural products with biological activity, the quality of traditional Chinese medicines (TCM) is the key to their clinical application. Fingerprints based on the types and contents of chemical components in TCM are an internationally recognized quality evaluation method but ignore the correlation between chemical components and efficacy. Through chemometric methods, the fingerprints represented by the chemical components of TCM were correlated with its pharmacodynamic activity results to obtain the spectrum–effect relationships of TCM, which can reveal the pharmacodynamic components information related to the pharmacodynamic activity and solve the limitations of segmentation of chemical components and pharmacodynamic research in TCM. In the 20th anniversary of the proposed spectrum–effect relationships, this paper reviews its research progress in the field of TCM, including the establishment of fingerprints, pharmacodynamic evaluation methods, chemometric methods and their practical applications in the field of TCM. Furthermore, the new strategy of spectrum–effect relationships research in recent years was also discussed, and the application prospects of this technology were discussed.

show abstract

Novel Natural Candidates for Replacing Synthetic Additives in Nutraceutical and Pharmaceutical Areas: Two Senna Species (S. alata (L.) Roxb. and S. occidentalis (L.) Link)

Yagi,

Cetiz,

Zengin

et al. 2024

Food Science & Nutrition

View full text Add to dashboard Cite

Senna alata (L.) Roxb. and Senna occidentalis (L.) Link (family Fabaceae) are commonly used in different systems of traditional medicine to treat ailments. The present study was designed to determine the phytoconstituents, antioxidant, enzyme inhibition, and antimicrobial activities of the methanolic extract from the leaves of these two Senna species. A total of 75 phenolic compounds belonging to dihydroxybenzoic acids, dihydroxycinnamic acids, flavonoid C‐glycosides, flavonoid O‐glycosides, flavonoid aglycones, anthraquinone glycosides, and anthraquinone aglycones were identified. Flavonoid C‐glycosides were only found in S. occidentalis while sennosides A, B, and C were only detected in S. alata. In line with its higher total phenolic and flavonoids contents, S. alata exerted significantly (p < 0.05) higher antiradical (2,2‐diphenyl‐1‐picrylhydrazy (DPPH) = 58.36 mg trolox equivalent (TE)/g; 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS) = 118.86 mg TE/g), ions reducing (cupric reducing antioxidant capacity (CUPRAC) = 93.85 mg TE/g; ferric reducing antioxidant power (FRAP) = 50.42 mg TE/g), and total antioxidant (1.39 mmol TE/g) activities than S. occidentalis. S. alata revealed significantly (p < 0.05) higher inhibitory effect against butyrylcholinesterase (1.67 mg galantamine equivalent (GALAE)/g), tyrosinase (45.07 mg KAE/g) 45.07 mg kojic acid equivalent (KAE)/g), α‐glucosidase (0.73 mmol acarbose equivalent (ACAE)/g), and α‐amylase (2.95 mmol ACAE/g) enzymes. Both species showed high antibacterial and antifungal activities with remarkable antifungal activity exerted by S. alata against Trichoderma viride (minimum inhibition concentration (MIC) 1 mg/mL), similar to that of Ketoconazole. The study utilized molecular docking, molecular mechanics Poisson–Boltzmann surface area (MM/PBSA) free energy calculations, and molecular dynamics simulations to evaluate the binding interactions between anthraquinone glycosides and various bacterial enzymes, including targets from Escherichia coli and Staphylococcus aureus. The findings suggest that compounds like sennoside A, sennoside B, and chrysophanol exhibit strong binding affinities, stable interactions, and potential as antimicrobial inhibitors, especially against vital bacterial proteins such as MurE and 30S ribosome S3. In conclusion, our findings underscore the biopharmaceutical potential of these two Senna species, suggesting their significance as sources of bioactive agents for health‐related applications.

show abstract

Validation of Sennae Folium specification grade classification based on UPLC-Q-TOF/MS spectrum-effect relationship

Cited by 10 publications

References 35 publications

Exploring Antioxidant and α-Glucosidase Inhibitory Activities in Mulberry Leaves (Morus alba L.) across Growth Stages: A Comprehensive Metabolomic Analysis with Chemometrics

Exploring Antioxidant and α-Glucosidase Inhibitory Activities in Mulberry Leaves (Morus alba L.) across Growth Stages: A Comprehensive Metabolomic Analysis with Chemometrics

Spectrum–Effect Relationships as an Effective Approach for Quality Control of Natural Products: A Review

Novel Natural Candidates for Replacing Synthetic Additives in Nutraceutical and Pharmaceutical Areas: Two Senna Species (S. alata (L.) Roxb. and S. occidentalis (L.) Link)

Contact Info

Product

Resources

About