Validation of the CULTEX® Radial Flow System for the assessment of the acute inhalation toxicity of airborne particles

Tsoutsoulopoulos, Amelie; Gohlsch, Katrin; Möhle, Niklas; Breit, Andreas; Hoffmann, Sebastian; Krischenowski, Olaf; Mückter, Harald; Gudermann, Thomas; Thiermann, Horst; Aufderheide, Michaela; Steinritz, Dirk

doi:10.1016/j.tiv.2019.03.020

Cited by 12 publications

(8 citation statements)

References 34 publications

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“…The use of human samples grown as a 3D pseudostratified respiratory epithelium at air liquid interface overcomes this issue by more closely mimicking in vivo conditions [105]. There also needs to be more consistency around the methods and protocols employed to generate vapours from ECS, ensuring the delivery and exposure is similar to human ECS inhalation [106]. To date there have been a wide variety of methods employed resulting in the varying concentrations and exposure time of ECS, in addition to the numerous ELs producing varying toxic compounds further adding to the complexity and inconsistency [43][44][45]107].…”

Section: Discussionmentioning

confidence: 99%

Vaping and lung cancer – A review of current data and recommendations

Bracken-Clarke¹,

Kapoor²,

Baird³

et al. 2021

Lung Cancer

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Lung cancer is the most common cause of cancer mortality worldwide and, while tobacco smoke remains the primary cause, there is increasing concern that vaping and E-cigarette use may also increase lung cancer risk. This review concentrates on the current data, scholarship and active foci of research regarding potential cancer risk and oncogenic mechanisms of vaping and lung cancer. Materials and methods: We performed a literature review of current and historical publications on lung cancer oncogenesis, vaping device/e-liquid contents and daughter products, molecular oncogenic mechanisms and the fundamental, potentially oncogenic, effects of electronic cigarette smoke/e-liquid products. Results: E-cigarette devices and vaping fluids demonstrably contain a series of both definite and probable oncogens including nicotine derivatives (e.g. nitrosnornicotine, nitrosamine ketone), polycyclic aromatic hydrocarbons, heavy metals (including organometal compounds) and aldehydes/other complex organic compounds. These arise both as constituents of the e-liquid (with many aldehydes and other complex organics used as flavourings) and as a result of pyrolysis/complex organic reactions in the electronic cigarette device (including unequivocal carcinogens such as formaldehydeformed from pyrolysis of glycerol). Various studies demonstrate in vitro transforming and cytotoxic activity of these derivatives. E-cigarette device use has been significantly increasingparticularly amongst the younger cohort and non-smokers; thus, this is an area of significant concern for the future. Conclusion: Although research remains somewhat equivocal, there is clear reason for concern regarding the potential oncogenicity of E-Cigarettes/E-Liquids with a strong basic and molecular science basis. Given lag times (extrapolating from tobacco smoke data) of perhaps 20 years, this may have significant future public health implications. Thus, the authors feel further study in this field is strongly warranted and consideration should be made for tighter control and regulation of these products.

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Section: Discussionmentioning

confidence: 99%

Vaping and lung cancer – A review of current data and recommendations

Bracken-Clarke¹,

Kapoor²,

Baird³

et al. 2021

Lung Cancer

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“…A variety of exposure systems have been developed for direct exposure of lung cells to test articles in their physiologically relevant forms (Aufderheide and Mohr 1999 ; Bitterle et al 2006 ; Lenz et al 2013 ; Thorne and Adamson 2013 ; Lacroix et al . 2018 ; Tsoutsoulopoulos et al 2019 ; Wang et al 2019a ; Wang et al 2019b ). These exposure systems range from those designed and used by individual research laboratories (Müller et al 2011 ; Gualerzi et al 2012 ; Amatngalim et al 2015 ; Ji et al 2017 ; Dwivedi et al 2018 ) to widely available systems that are commercially marketed (Aufderheide and Mohr 1999 ; Okuwa et al 2010 ; Li et al 2012 ; Tsoutsoulopoulos et al 2019 ).…”

Section: Exposure Systems Used With Ali Airway Culturesmentioning

confidence: 99%

“… 2018 ; Tsoutsoulopoulos et al 2019 ; Wang et al 2019a ; Wang et al 2019b ). These exposure systems range from those designed and used by individual research laboratories (Müller et al 2011 ; Gualerzi et al 2012 ; Amatngalim et al 2015 ; Ji et al 2017 ; Dwivedi et al 2018 ) to widely available systems that are commercially marketed (Aufderheide and Mohr 1999 ; Okuwa et al 2010 ; Li et al 2012 ; Tsoutsoulopoulos et al 2019 ). Gases, NPs, aerosols, particles, and complex aerosols (e.g., CS and diesel exhaust) have been tested using specialized systems that expose the apical surface of ALI airway cultures (Aufderheide and Mohr 2004 ; Aufderheide and Gressmann 2007 ; Okuwa et al 2010 ; Lenz et al 2013 ; Nara et al 2013 ; Lenz et al 2014 ; Wang et al 2019a ; Wang et al 2019b ).…”

Section: Exposure Systems Used With Ali Airway Culturesmentioning

confidence: 99%

“…CFD was also used to determine particle number and mass distribution of the test articles in this study. CULTEX® RFS Exposure Systems have been used for toxicity assessment of CS, NPs, and gases (Aufderheide et al 2011 ; Aufderheide et al 2013 ; Rach et al 2014 ; Steinritz et al 2013 ; Tsoutsoulopoulos et al 2019 ). A modified version of the exposure module was used for analyzing the mutagenic potency of airborne substances in the AMES assay (Aufderheide et al , 2011 ).…”

Section: Exposure Systems Used With Ali Airway Culturesmentioning

confidence: 99%

“…A modified version of the exposure module was used for analyzing the mutagenic potency of airborne substances in the AMES assay (Aufderheide et al , 2011 ). In a recent study, the acute toxicity of a set of 20 pre-selected test articles was assessed using the CULTEX® RFS and compared with in vivo reference data (Tsoutsoulopoulos et al 2019 ). This study demonstrates a high level of concordance, specificity, and sensitivity as well as good intra/inter-laboratory reproducibility for the in vitro findings.…”

Section: Exposure Systems Used With Ali Airway Culturesmentioning

confidence: 99%

See 2 more Smart Citations

Invited review: human air-liquid-interface organotypic airway tissue models derived from primary tracheobronchial epithelial cells—overview and perspectives

Cao

Coyle

Xiong

et al. 2020

In Vitro Cell.Dev.Biol.-Animal

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Air−Liquid Interface Cultures of the Healthy and Diseased Human Respiratory Tract: Promises, Challenges, and Future Directions

Baldassi

Gabold

Merkel

2021

Advanced NanoBiomed Research

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Air−liquid interface (ALI) culture models currently represent a valid instrument to recreate the typical aspects of the respiratory tract in vitro in both healthy and diseased state. They can help reducing the number of animal experiments, and hence support the 3R principle. This review discusses ALI cultures and co‐cultures derived from immortalized as well as primary cells, which are used to study the most common disorders of the respiratory tract, in terms of both pathophysiology and drug screening. The article displays ALI models used to simulate inflammatory lung diseases such as chronic obstructive pulmonary disease (COPD), asthma, cystic fibrosis, lung cancer, and viral infections. It also focuses on ALI cultures described in literature studying respiratory viruses such as SARS‐CoV‐2 causing the global Covid‐19 pandemic at the time of writing this review. Additionally, commercially available models of ALI cultures are presented. Ultimately, the aim of this review is to provide a detailed overview of ALI models currently available and to critically discuss them in the context of the most prevalent diseases of the respiratory tract.

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Validation of the CULTEX® Radial Flow System for the assessment of the acute inhalation toxicity of airborne particles

Cited by 12 publications

References 34 publications

Vaping and lung cancer – A review of current data and recommendations

Vaping and lung cancer – A review of current data and recommendations

Invited review: human air-liquid-interface organotypic airway tissue models derived from primary tracheobronchial epithelial cells—overview and perspectives

Air−Liquid Interface Cultures of the Healthy and Diseased Human Respiratory Tract: Promises, Challenges, and Future Directions

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