Validation of the low dose short insulin tolerance test for evaluation of insulin sensitivity

Gelding, Susan; Robinson, Stephen T.; Lowe, Stephen L.; Niththyananthan, R.; Johnston, D. G.

doi:10.1111/j.1365-2265.1994.tb03012.x

Cited by 76 publications

(55 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…After all haemodynamic measurements had been completed, we measured insulin sensitivity using the low-dose short insulin tolerance test [17] . Before administering an insulin bolus, baseline blood specimens were taken for fasting glucose and insulin levels together with neurohormonal analysis.…”

Section: Insulin Sensitivitymentioning

confidence: 99%

Endogenous insulin and insulin sensitivity. An important determinant of skeletal muscle blood flow in chronic heart failure?

Houghton

Harrison

Perry

et al. 1998

European Heart Journal

View full text Add to dashboard Cite

Section: Insulin Sensitivitymentioning

confidence: 99%

Endogenous insulin and insulin sensitivity. An important determinant of skeletal muscle blood flow in chronic heart failure?

Houghton

Harrison

Perry

et al. 1998

European Heart Journal

View full text Add to dashboard Cite

“…This was assessed by the short insulin tolerance test (SITT) (6). Subjects were studied in the metabolic day unit after an overnight fast of 10-12 h and asked to observe their usual diet and abstain from alcohol or strenuous exercise for the previous 3 days.…”

Section: Assessment Of Insulin Sensitivitymentioning

confidence: 99%

Sustained endogenous glucose production, diminished lipolysis and non-esterified fatty acid appearance and oxidation in non-obese women at high risk of type 2 diabetes

Forbes¹,

Robinson²,

Dungu³

et al. 2006

eur j endocrinol

View full text Add to dashboard Cite

Objective: To evaluate early defects in glucose production, lipolysis and fatty acid oxidation in nonobese, normally glucose tolerant women, who are nevertheless at risk of type 2 diabetes. Methods: Ten women with previous gestational diabetes (pGDM) and ten controls were studied in two 4 h infusions of stable isotopes 6,6-2 H 2 -glucose, 1-13 C-palmitate, and 1,1,2,3,3-2 H 5 -glycerol with and without infusion of adrenaline. Fatty acid oxidation was quantified using indirect calorimetry and 13 CO 2 measurements. Insulin sensitivity was evaluated using the short insulin tolerance test. Results: The pGDM and control women were non-obese and carefully matched for body mass index and fat mass. Whole body insulin sensitivity and basal insulin concentrations did not differ significantly but basal glucose concentrations were increased in women with pGDM. During a 0.9% saline infusion, glucose appearance was not significantly different at the first (90-120 min) and second (210-240 min) steady states. However, glucose appearance decreased in controls but was maintained in the pGDM women (K0.33G0.02 vs K0.03G0.08 mg/kg per min; PZ0.004). Basal glycerol appearance (0.27G0.02 vs 0.38G0.03 mg/kg per min; PZ0.02), palmitate appearance (0.74G0.09 vs 1.05G0.09 mg/kg per min; PZ0.03) and palmitate oxidation (0.07G0.01 vs 0.10G0.01 mg/kg per min; PZ0.03) were lower in the pGDM women. During the adrenaline infusion, changes in glucose, glycerol and palmitate concentrations and kinetics were similar in both groups. Conclusions: Sustained glucose production during fasting is an early abnormality in non-obese subjects at risk of type 2 diabetes. Lipolysis and non-esterified fatty acid appearance and oxidation are diminished, suggesting an increased tendency to store fat. The observations are not readily attributable to differences in insulin or catecholamine sensitivity. 155 469-476 European Journal of Endocrinology

show abstract

“…This difference in prevalence of IR could be explained by fact that the pathogenesis of IR involves the interplay of both genetic (often polygenic in nature) and environmental factors [24]. Nonetheless, several other factors have been implicated in the aetiology of IR, namely prenatal factors, ethnicity, diet, puberty, and sedentary lifestyles.…”

Section: Discussionmentioning

confidence: 99%

Insulin Sensitivity and Gynaecological Features of Infertile Cameroonian Females with Polycystic Ovary Syndrome: A Cross-Sectional Study

Dohbit¹,

Sobngwi²,

Kemfang³

et al. 2017

OJOG

View full text Add to dashboard Cite

Background: Polycystic ovary syndrome (PCOS), characterized by ovulatory dysfunction, polycystic ovary (PCO), hyperandrogenism and insulin resistance is the commonest endocrine disorder in women of reproductive age. It is an intriguing pathology that involves the perpetuation of a vicious circle with reproductive, endocrine and metabolic components. We aimed to assess the reproductive features and insulin sensitivity (IS) in infertile women with or without PCOS. Materials and Methods: We carried out a cross-sectional analytic study at the outpatient Obstetrics and Gynaecology Department of the Yaounde Gyneco-obstetric and Pediatrics Hospital, Cameroon from September 1 st 2012 to March 31 st 2013 giving total study duration of 07 months.Laboratory analyses were carried out at the National Obesity Centre (NOC) of the Yaounde Central Hospital, Cameroon. Results: Overall, 36 infertile females were enrolled, which included 15 diagnosed cases of PCOS according to Rotterdam consensus meeting of 2003 and 21 non PCOS subjects as control.PCOS women were younger than non PCOS women (28.8 ± 5.5 vs. 35.0 ± 4.2 years; p = 0.0004). The majority of the women in the PCOS group were spaniomenorrheic (11/15), and ultrasonographic findings were typical of PCOS. Hirsutism score was higher in the PCOS group with a median of 9 (7 -13).Insulin sensitivity was impaired in two-thirds of the study population, with 12 women found to be insulin resistant (6 PCOS, 6 non PCOS), 12 patients had Apart from blood glucose levels (p = 0.007), all other anthropometric and biological parameters were not significant. Spearman's correlation identified fasting plasma glucose and total cholesterol as factors associated with insulin sensitivity in females with PCOS. Impaired fasting glucose was observed in 13 patients with 08 from the PCOS group. Conclusion: We conclude that young age, spaniomenorrhea and hirsutism are common findings in PCOS. Furthermore, our findings suggest that PCOS may be more of systemic metabolic disease than solely a purely gynecologic disorder as described hitherto. Despite normal fasting plasma glucose levels, a good proportion of these women has impaired insulin sensitivity and it is associated with a metabolic syndrome.

show abstract

Validation of the low dose short insulin tolerance test for evaluation of insulin sensitivity

Abstract: The short insulin tolerance test employing 0.05 units/kg insulin is a safe, valid and reproducible method for the assessment of insulin sensitivity.

Cited by 76 publications

References 8 publications

Endogenous insulin and insulin sensitivity. An important determinant of skeletal muscle blood flow in chronic heart failure?

Endogenous insulin and insulin sensitivity. An important determinant of skeletal muscle blood flow in chronic heart failure?

Sustained endogenous glucose production, diminished lipolysis and non-esterified fatty acid appearance and oxidation in non-obese women at high risk of type 2 diabetes

Insulin Sensitivity and Gynaecological Features of Infertile Cameroonian Females with Polycystic Ovary Syndrome: A Cross-Sectional Study

Contact Info

Product

Resources

About