Validation of tissue microarray technology in malignant peripheral nerve sheath tumours

Abstract

This study indicates that the TMA technique can be used in the IHC study of MPNSTs, even with the use of heterogeneous markers such as S-100 protein.

Sporadic primary malignant intracerebral nerve sheath tumors: case report and literature review

“…Third, several reports have documented syncytia formation and islands of epithelioid tumor cells, as was the case in the specimen analyzed here. Variable expression of S-100 in MPNST has been previously shown and in our experience very low expression is a common immunohistochemical finding [14].…”

Evaluation of Bcl-2, Bcl-x and Cleaved Caspase-3 in Malignant Peripheral Nerve Sheath Tumors and Neurofibromas

“…All the cases were used in previous studies (Cunha et al 2003(Cunha et al , 2008(Cunha et al , 2009. The samples of the tumors were included in two tissue microarray (TMA) paraffin blocks.…”

“…After dewaxing, proteins were demonstrated by IHC, using the protocol described in a previous study (Cunha et al 2009). Sections were incubated overnight at 4°C with a 1:200 dilution of the primary antibody anti-Bcl-2 (clone 124; Dako Corporation, USA), with a 1:25 dilution of the primary antibody anti-Bcl-x (clone A35-10; Dako Corporation, USA), and with a 1:600 dilution -, information not available (the tumor resection was performed in other institution); sd, standard deviation *, in each case, at least 30 fields were analyzed and the mean value of the sum of mitotic figures in each 10 high-power fields is showed in the table.…”

“…Following the independent evaluation of the immunohistochemical staining, if there were any divergence between the pathologists, the glass slides were discussed at a separate evaluation in order to reach a consensus on the evaluation. For the cases classified as positive, quantification of Bcl-2, Bcl-x and CC3 immuno staining was performed by computerized digital image analysis (ImagePro Plus software v4.5; Media Cybernetics), as previously described (Cunha et al 2009), and expressed as positivity index (PI), defined as positive area divided by total tissue area.…”

Validation of a low-cost modified technique for constructing tissue microarrays for canine mammary tumor analysis

“…Conventional paraffin blocks are also representative of only a small fraction of the lesion or the organ of origin [3][4][5]. One must keep in mind that conventional sections are also samples selected based on a macroscopic examination, and even if the entire tumor was embedded in paraffin, it will not be examined in its entirety [3].…”