Validation of transparent and flexible neural implants for simultaneous electrophysiology, functional imaging, and optogenetics

Koschinski, Lina; Lenyk, Bohdan; Jung, Marie; Lenzi, Irene; Kampa, Björn; Mayer, Dirk; Offenhäusser, Andreas; Musall, Simon; Rincón Montes, Viviana

doi:10.1039/d3tb01191g

Cited by 2 publications

(2 citation statements)

References 66 publications

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“…Another approach has been described for cell stimulation that may be used with a three-electrode system, in which cells attach directly to the working electrode [31]. The three-electrode system consists of a working electrode, a counter electrode, and a reference electrode [37]. The system is the most common electrochemical system for accurately measuring potential and current.…”

Section: Electrical Stimulationmentioning

confidence: 99%

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Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review

Tominami,

Kudo,

Noguchi

et al. 2024

IJMS

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PC12 cells, which are derived from rat adrenal pheochromocytoma cells, are widely used for the study of neuronal differentiation. NGF induces neuronal differentiation in PC12 cells by activating intracellular pathways via the TrkA receptor, which results in elongated neurites and neuron-like characteristics. Moreover, the differentiation requires both the ERK1/2 and p38 MAPK pathways. In addition to NGF, BMPs can also induce neuronal differentiation in PC12 cells. BMPs are part of the TGF-β cytokine superfamily and activate signaling pathways such as p38 MAPK and Smad. However, the brief lifespan of NGF and BMPs may limit their effectiveness in living organisms. Although PC12 cells are used to study the effects of various physical stimuli on neuronal differentiation, the development of new methods and an understanding of the molecular mechanisms are ongoing. In this comprehensive review, we discuss the induction of neuronal differentiation in PC12 cells without relying on NGF, which is already established for electrical, electromagnetic, and thermal stimulation but poses a challenge for mechanical, ultrasound, and light stimulation. Furthermore, the mechanisms underlying neuronal differentiation induced by physical stimuli remain largely unknown. Elucidating these mechanisms holds promise for developing new methods for neural regeneration and advancing neuroregenerative medical technologies using neural stem cells.

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Section: Electrical Stimulationmentioning

confidence: 99%

“…It is primarily used in the field of neural implants, which are implantable electronic devices that include microelectrode arrays designed to electrically connect the devices to the nervous system. The neural implants can stimulate neural activity in various neural tissues [37].…”

Section: Electrical Stimulationmentioning

confidence: 99%

Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review

Tominami,

Kudo,

Noguchi

et al. 2024

IJMS

View full text Add to dashboard Cite

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Transparent, flexible graphene-ITO-based neural microelectrodes for simultaneous electrophysiology recording and calcium imaging of intracortical neural activity in freely moving mice

Pei,

Yuan,

et al. 2024

Preprint

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To understand of the complex dynamics of neural activity in the brain across various temporal and spatial scales, it is crucial to record intracortical multimodal neural activity by combining electrophysiological recording and calcium imaging techniques. This poses significant constraints on the geometrical, mechanical and optical properties of the electrodes. Here, transparent flexible graphene-ITO-based neural microelectrodes with small feature sizes are developed and validated for simultaneous electrophysiology recording and calcium imaging in the hippocampus of freely moving mice. A micro-etching technique and an oxygen plasma pre-treating method are introduced to facilitate large-area graphene transfer and establish stable low-impedance contacts between graphene and metals, leading to the batch production of high-quality microelectrodes with interconnect widths of 10 µm and recording sites diameters of 20 µm. These electrodes exhibit appropriate impedance and sufficient transparency in the field pf view, enabling simultaneous recording of intracortical local field potentials and even action potentials along with calcium imaging in freely moving mice. Both types of electrophysiological signals are found to correlate with calcium activity. This proof-of-concept work demonstrates that transparent flexible graphene-ITO-based neural microelectrodes are promising tools for multimodal neuroscience research.

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Validation of transparent and flexible neural implants for simultaneous electrophysiology, functional imaging, and optogenetics

Abstract: The combination of electrophysiology and neuroimaging methods allows the simultaneous measurement of electrical activity signals with calcium dynamics from single neurons to neuronal networks across distinct brain regions in vivo....

Cited by 2 publications

References 66 publications

Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review

Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review

Transparent, flexible graphene-ITO-based neural microelectrodes for simultaneous electrophysiology recording and calcium imaging of intracortical neural activity in freely moving mice

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