Validation of uniformity-optimized irradiance distribution on a well-plate platform from a light-emitting-diode array

Abstract

The computational optimization of irradiance distribution uniformity has been conducted in several studies to obtain the evenness of photoresponses on an irradiated surface using light-emitting-diode (LED) arrays. However, there has been little discussion on the precision of predictive simulations. This study aims to validate the simulated irradiance predicted by a mathematical model on the working area of a six-well plate and investigate the spatial consistency of the photobleaching of methylene blue and IR-820 photosensitizers on the bottom of the different wells illuminated by using the local-search-optimized LED configurations. The validation signified the negative deviation of both the measured irradiance and irradiance uniformity as compared to the simulated data. Despite the coefficients of variation observed as low as 1.9% and 7.4% for red-light and infrared irradiance, respectively, the photobleaching responses were found to be spatially diverse. The implications of this study are opportunities for further enhancements to the predictability of the simulations for the design of prospective illumination setups.

Wearable Surface‐Lighting Micro‐Light‐Emitting Diode Patch for Melanogenesis Inhibition

“…[55][56][57][58] Figure 3C shows a temperature measurement method using a contact thermometer during surface-lighting irradiation on a human wrist. Figure 3D; Figure S14, Supporting Information, exhibits temperature changes of the 30 × 30 SμLEDs at five different input currents (20,40,80, 100, and 120 mA). Despite 120 mA current injection, the device temperature remained below 39 °C for 10 min.…”

“…[1] Various treatments including physical, chemical, and optical with ≈2 cm radiation distance leads to non-uniform irradiation, requiring more vertical distance to evenly cover the 3D faces with the light. [20] While light uniformity can be improved by increasing vertical distance, the optical power density at the skin surface is significantly reduced by the long radiation distance and severe diffuse reflection. [21,22] Some researchers reported that only 10% of incident light could penetrate into a skin model when irradiated with a 630 nm light source at 2 cm distance.…”