Validation of use of annular once-punched filter paper bloodspot samples for repeat lead testing

El-Hajjar, Dana F.; Swanson, Katherine H.; Landmark, James D.; Stickle, Douglas F.

doi:10.1016/j.cca.2006.09.020

Cited by 21 publications

(18 citation statements)

References 10 publications

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“…The Pb concentrations determined in DBS have been shown to be independent from the volume spotted and from the site of punching (excluding the area near the perimeter, where concentrations are higher owing to a higher amount of red blood cells). Moreover, a good correlation was found between Pb concentrations in DBS and those in venous blood (El-Hajjar et al, 2007, Resano et al, 2007. DBS obtained from subjects with strongly deviating haematocrit values, however, may give rise to discordant results (El-Hajjar et al, 2007).…”

Section: Elemental Analysismentioning

confidence: 91%

“…Moreover, a good correlation was found between Pb concentrations in DBS and those in venous blood (El-Hajjar et al, 2007, Resano et al, 2007. DBS obtained from subjects with strongly deviating haematocrit values, however, may give rise to discordant results (El-Hajjar et al, 2007). Apart from Pb, also other toxic metals, as well as elements of clinical or forensic interest, have been determined (or have been shown to be detectable) in DBS, including As, Ba, Be, Bi, Ca, Cd, Co, Cr, Cs, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Rb, S, Sb, Se, Tl, V and Zn (Lombeck et al, 1989, Chaudhuri et al, 2009, Langer et al, 2010, Hsieh et al, 2011, Shlosberg et al, 2011b.…”

Section: Elemental Analysismentioning

confidence: 91%

“…Indeed, given the ubiquitous presence of Pb in the environment, special care has to be taken to avoid contamination at every step, from paper handling, sampling, and drying, over transport to analysis. More specifically, falsely elevated Pb concentrations may result from contamination by Pb present on the skin (thus necessitating suitable cleansing before sampling) and/or by improper paper handling (El-Hajjar et al, 2007, Moyer et al, 1999, Moyer et al, 2000. In contrast to a controlled clinical environment, in which the issue of contamination can be dealt with from sampling to analysis, one has to be aware that DBS sampling 'on-field', with less control on preanalytical variables, potentially suffers from an increased risk of contamination.…”

Section: Elemental Analysismentioning

confidence: 99%

See 2 more Smart Citations

Dried blood spots in toxicology: from the cradle to the grave?

Stove

Ingels

Kesel

et al. 2012

Critical Reviews in Toxicology

146

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About a century after its first described application by Ivar Bang, the potential of sampling via dried blood spots (DBS) as an alternative for classical venous blood sampling is increasingly recognized. Perhaps best known is the use of DBS in newborn screening programs, ignited by the hallmark paper by Guthrie and Susi half a century ago. However, it is only recently that both academia and industry have recognized the many advantages that DBS sampling may offer for bioanalytical purposes, as reflected by the strong increase in published reports during the last few years. Currently, major DBS applications include newborn screening for metabolic disorders, epidemiological surveys (e.g. HIV monitoring), therapeutic drug monitoring (TDM), as well as toxicology. In this review, we provide a comprehensive overview of the distinct subdisciplines of toxicology for which DBS sampling has been applied. DBS sampling for toxicological evaluation has been performed from birth until autopsy, aiming at the assessment of therapeutic drugs, drugs of abuse, environmental contaminants, toxins, as well as (trace) elements, with applications situated in fields as toxicokinetics, epidemiology and environmental and forensic toxicology. We discuss the strengths and limitations of DBS in the different subdisciplines and provide future prospects for the use of this promising sampling technique in toxicology.

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Section: Elemental Analysismentioning

confidence: 91%

Section: Elemental Analysismentioning

confidence: 91%

Section: Elemental Analysismentioning

confidence: 99%

See 1 more Smart Citation

Dried blood spots in toxicology: from the cradle to the grave?

Stove

Ingels

Kesel

et al. 2012

Critical Reviews in Toxicology

146

View full text Add to dashboard Cite

show abstract

“…DBS used for analysis were prepared in the laboratory by bolus addition of 40 μL Li-heparin whole blood to Whatman 903 cards. After drying (24 h), total bloodspot area was determined by image analysis using Visual Basic [8]. Briefly, a 200-dpi bitmap image of the DBS was obtained using a desktop scanner.…”

Section: Methodsmentioning

confidence: 99%

“…One interesting property of filter paper DBS is that there is usually a significantly higher density of RBCs at the DBS perimeter in comparison to the interior [8,9]. The accrual of RBCs at the perimeter is often readily visible in DBS.…”

Section: Introductionmentioning

confidence: 96%

Estimation of hematocrit in filter paper dried bloodspots by potassium measurement: advantage of use of perimeter ring samples over circular center sub-punch samples

Rufail

McCloskey

Stickle

2016

Clinical Chemistry and Laboratory Medicine (CCLM)

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Effects of spot size on biomarker levels of field‐collected dried blood spots: A new algorithm for exact dried blood spot size measurement

Groh

Weiss

Börsch‐Supan³

et al. 2022

American J Hum Biol

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Objectives: The quality of blood values analyzed from survey-collected dried blood spot (DBS) samples is affected by fieldwork conditions, particularly spot size. We offer an image-based algorithm that accurately measures the area of field-collected DBS and we investigate the impact of spot size on the analyzed blood marker values.Methods: SHARE, a pan-European study, collected 24 000 DBS samples in 12 countries in its sixth wave. Our new algorithm uses photographs of the DBS samples to calculate the number of pixels of the blood-covered area to measure the spot sizes accurately. We ran regression models to examine the association of spot size and seven DBS analytes. We then compared the application of our new spot-size measures to common spot-size estimation. Results: Using automated spot-size measurement, we found that spot size has a significant effect on all markers. Smaller spots are associated with lower measured levels, except for HbA1c, for which we observe a negative effect. Our precisely measured spot sizes explain substantially more variance of DBS analytes compared to commonly used spot-size estimation. Conclusion:The new algorithm accurately measures the size of field-collected DBS in an automated way. This methodology can be applied to surveys even with very large numbers of observations. The measured spot sizes improve the accuracy of conversion formulae that translate blood marker values derived from DBS into venous blood values. The significance of the spot-size effects on biomarkers in DBS should also incentivize the improvement of fieldwork training and monitoring.

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Validation of use of annular once-punched filter paper bloodspot samples for repeat lead testing

Cited by 21 publications

References 10 publications

Dried blood spots in toxicology: from the cradle to the grave?

Dried blood spots in toxicology: from the cradle to the grave?

Estimation of hematocrit in filter paper dried bloodspots by potassium measurement: advantage of use of perimeter ring samples over circular center sub-punch samples

Effects of spot size on biomarker levels of field‐collected dried blood spots: A new algorithm for exact dried blood spot size measurement

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