Validations of time-resolved fluoroimmunoassays for urinary estrone 3-glucuronide and pregnanediol 3-glucuronide

Kesner, James S.; Knecht, Edwin A.; Krieg, Edward F.; Barnard, G.; Mikola, Heikki; Kohen, Förtüne; Gani, M.; Coley, John

doi:10.1016/0039-128x(94)90029-9

Cited by 42 publications

(20 citation statements)

References 26 publications

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“…A time resolved fluorescence immunoassay for the measurement of E3G and P3G in urine was used which had low interassay and intraassay variation (,6%). 18 All urinary hormone measurements were creatinine standardised. 19 …”

Section: Analysis Of Specimensmentioning

confidence: 99%

Factors affecting vaginal pH levels among female adolescents attending genitourinary medicine clinics

Brabin

Roberts²,

Fairbrother³

et al. 2005

Sexually Transmitted Infections

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Objectives: Vaginal pH is related to hormonal status, and adolescents experience disturbed hormonal patterns following menarche. We assessed hormonal factors and risk of abnormal vaginal pH and bacterial vaginosis (BV) among adolescents attending genitourinary medicine (GUM) clinics. Methods: In a cross sectional study adolescents within 5 years of menarche, (17 years, or with oligoamenorrhoea were recruited. Vaginal pH and BV were assessed and among those not using hormonal contraceptives, estrone-3-glucuronide (E3G) and pregnanediol-3a-glucuronide (P3G) concentrations were measured. Results: Among 102 adolescents, 59.8% (61) had a high vaginal pH (.4.5), which was higher than the prevalence of BV, detected in 33% (34). No association was found between presence of sexually transmitted infections (STI) and vaginal pH. In logistic regression, after controlling for BV and condom use, vaginal pH was positively associated with cervical ectopy (OR = 2.5; 95% CI 1.0 to 6.6, p = 0.05) and STI treatment history (OR = 2.5; 95% CI 0.9 to 6.5, p = 0.07), and negatively associated with use of DepoProvera (OR = 0.1; 95% CI 0.03 to 0.6, p = 0.003) and recent onset (,12 months) of sexual activity (OR = 0.2; 95% CI 0.1 to 0.7, p = 0.004). Among 23 adolescents not using hormonal contraceptives, a high pH occurred more often in abnormal compared to normal menstrual cycles (OR = 10.8; 95% CI 1.4 to 85.4; p = 0.026). E3G concentrations were inversely correlated with vaginal pH in the follicular phase (Spearman: r = 0.51; p = 0.024). Conclusions: Ectopy and abnormal menstrual cycles are common features of adolescence. Their presence is associated with increased risk of abnormal pH, and may also predispose to BV.

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Section: Analysis Of Specimensmentioning

confidence: 99%

Factors affecting vaginal pH levels among female adolescents attending genitourinary medicine clinics

Brabin

Roberts²,

Fairbrother³

et al. 2005

Sexually Transmitted Infections

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“…We assayed urinary LH using a commercial noncompetitive, two-site, timeresolved immunofluorometric assay (Kesner et al 1998(Kesner et al , 1994a. Detailed characteristics of these assays, including specificity, sensitivity, and precision, are described in their respective references (Jaffe 1886;Kesner et al 1994aKesner et al , 1994bKesner et al , 1998. We measured creatinine spectrophotometrically (Jaffe 1886) and divided each urinary endocrine value by the creatinine concentration to adjust for urine dilution.…”

Section: Methodsmentioning

confidence: 99%

“…We assayed the major urinary metabolites of estradiol and progesterone (i.e., E 1 3G and Pd3G) using competitive, double-antibody time-resolved fluoroimmunoassays (Kesner et al 1994b). We assayed urinary LH using a commercial noncompetitive, two-site, timeresolved immunofluorometric assay (Kesner et al 1998(Kesner et al , 1994a.…”

Section: Methodsmentioning

confidence: 99%

Evidence of reproductive endocrine effects in women with occupational fuel and solvent exposures.

Reutman

LeMasters

Knecht

et al. 2002

Environ Health Perspect

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Hydrocarbons (HCs) found in fuels and solvents are ubiquitous in the environment, yet we know little about their effects on the endocrine system. The objective of this study was to assess the potential reproductive endocrine effects of low-dose HCs encountered by female U.S. Air Force personnel with fuel (primarily JP-8 jet fuel) and solvent exposures (n = 63). We estimated the internal dose of HCs in fuels and solvents by measuring their levels in exhaled breath, including the sum of aliphatic HCs (C6H14-C16H34) and the sum of aromatic HCs (benzene, ethylbenzene, toluene, and m,p,o-xylenes). Adverse outcome measures included urinary endocrine markers that have been associated with nonconceptive (vs. conceptive) menstrual cycles in ovulatory women: lower preovulatory luteinizing hormone (LH) and mid-luteal phase pregnanediol 3-glucuronide (Pd3G) and estrone 3-glucuronide, and higher follicle phase Pd3G. We also obtained reproductive and exposure information from baseline questionnaires and daily diaries. Toluene was the most frequently found analyte in the breath, with values up to 52.0 ppb, and benzene breath levels were up to 97.5 ppb. Regression analysis revealed that preovulatory LH levels were significantly lower (p = 0.007) among women whose total aliphatic HC levels were above the median. The relationship between elevated aliphatic HC exposure and lowered preovulatory LH levels in the present study suggests that compounds in fuels and some solvents may act as reproductive endocrine disruptors. Confirmation of these findings is needed, not only to determine if fuel and solvent exposure may impact other LH-dependent physiologic functions but also to examine effects of fuels and solvents on conception.

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“…Our first objective was to evaluate the performance of urinary PDG and E1C EIAs using monoclonal antibodies. The anti-E1C monoclonal antibody used in the present report has been well characterized (20 ) and used in a fluoroimmunoassay (10,11 ).…”

mentioning

confidence: 99%

Urinary Estrone Conjugate and Pregnanediol 3-Glucuronide Enzyme Immunoassays for Population Research

O’Connor

Brindle²,

Holman³

et al. 2003

Clinical Chemistry

102

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Background:Monitoring of reproductive steroid hormones at the population level requires frequent measurements, hormones or metabolites that remain stable under less than ideal collection and storage conditions, a long-term supply of antibodies, and assays useful for a range of populations. We developed enzyme immunoassays for urinary pregnanediol 3-glucuronide (PDG) and estrone conjugates (E1Cs) that meet these criteria. Methods: Enzyme immunoassays based on monoclonal antibodies were evaluated for specificity, detection limit, parallelism, recovery, and imprecision. Paired urine and serum specimens were analyzed throughout menstrual cycles of 30 US women. Assay application in different populations was examined with 23 US and 42 Bangladeshi specimens. Metabolite stability in urine was evaluated for 0 -8 days at room temperature and for 0 -10 freeze-thaw cycles. Results: Recoveries were 108% for the PDG assay and 105% for the E1C assay. Serially diluted specimens exhibited parallelism with calibration curves in both assays. Inter-and intraassay CVs were <11%. Urinary and serum concentrations were highly correlated: r ‫؍‬ 0.93 for E1C-estradiol; r ‫؍‬ 0.98 for PDG-progesterone.

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Validations of time-resolved fluoroimmunoassays for urinary estrone 3-glucuronide and pregnanediol 3-glucuronide

Cited by 42 publications

References 26 publications

Factors affecting vaginal pH levels among female adolescents attending genitourinary medicine clinics

Factors affecting vaginal pH levels among female adolescents attending genitourinary medicine clinics

Evidence of reproductive endocrine effects in women with occupational fuel and solvent exposures.

Urinary Estrone Conjugate and Pregnanediol 3-Glucuronide Enzyme Immunoassays for Population Research

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