Distributed fibre optical sensing (DFOS) is increasingly used in civil engineering research. For reinforced concrete structures, almost continuous information concerning the deformations of embedded reinforcing bars can be obtained. This information enables the validation of basic and conventional assumptions in the design and modelling of reinforced concrete, particularly regarding the interaction of concrete and reinforcing bars. However, this relatively new technology conceals some difficulties, which may lead to erroneous interpretations. This paper (i) discusses the selection of sensing fibres for reinforced concrete instrumentation, accounting for strain gradients and local anomalies caused by stress concentrations due to the reinforcing bar ribs; (ii) describes suitable methods for sensor installation, strain acquisition and post-processing of the data, as well as determining and validating structurally relevant entities; and (iii) presents the results obtained by applying DFOS with these methods in a variety of experiments. The analysed experiments comprise a reinforced concrete tie, a pull-out test under cyclic load, and a flexural member in which the following mechanical relevant quantities are assessed: the initial strain state in reinforcing bars, normal and bond shear stresses, deflections as well as forces. These applications confirm the benefit of DFOS to better understand the bond behaviour, but also demonstrate that its application is intricate and the results may lead to erroneous conclusions unless evaluated meticulously.