Validity and application of immunoturbidimetric and enzyme‐linked immunosorbent assays for the measurement of adiponectin concentration in ponies

Menzies‐Gow, N. J.; Knowles, E. J.; Rogers, Ian Munro; Rendle, David

doi:10.1111/evj.12960

Cited by 10 publications

(13 citation statements)

References 19 publications

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“…Therefore, the ELISA was revalidated as part of this study. Equine samples were separately pooled from ponies with expected high and low adiponectin concentrations based on their post-prandial insulin concentration [30]. Assay precision was determined by the coefficient of variation (CV) among six replicates of the pooled ‘high’ sample.…”

Section: Methodsmentioning

confidence: 99%

The cresty neck score is an independent predictor of insulin dysregulation in ponies

et al. 2019

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Generalized obesity, regional adiposity, hyperinsulinemia and hypertriglyceridemia are all potential indicators of equine metabolic syndrome (EMS). This study aimed to assess the relationship between morphometric measurements of body condition and metabolic hormone concentrations in ponies, with and without a neck crest or generalised obesity. Twenty-six ponies were assigned a body condition score (BCS) and cresty neck score (CNS). Height, girth, and neck measurements were taken. An oral glucose test (OGT; 0.75g dextrose/kg BW) was performed and blood samples collected prior to and 2 hours post dosing. Basal blood samples were analysed for blood glucose, serum insulin, triglyceride and leptin, and plasma HMW adiponectin concentrations. Post-prandial samples were analysed for serum insulin concentration. The ponies were grouped as having a) a normal to fleshy body status (BCS ≤7 and CNS ≤2; n = 10); b) having a high CNS, but without generalised obesity (BCS ≤7 and CNS ≥3; n = 11), or c) being obese (BCS ≥8 and CNS ≥1; n = 5). Responses to the OGT indicated that both normal and insulin-dysregulated ponies were included in the cohort. Post-prandial serum insulin was positively associated with CNS (P<0.035) and ponies with a CNS ≥ 3 had 5 times greater odds of being insulin-dysregulated. The high CNS group had a greater insulin response to the OGT than those in the normal/fleshy group (P = 0.006), whereas obese ponies did not differ from the other two groups. Basal HMW adiponectin was negatively correlated with post-prandial insulin concentrations (r = -0.5, P = 0.009), as well as being decreased in the group with a high CNS, compared to the obese group (P = 0.05). Cresty neck score was more predictive of insulin dysregulation than BCS, and this may be relevant to the diagnosis of EMS. Adiponectin may also be a measure of insulin dysregulation that is independent of body condition.

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Section: Methodsmentioning

confidence: 99%

The cresty neck score is an independent predictor of insulin dysregulation in ponies

et al. 2019

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“…In horses, both HMW and total adiponectin are associated with obesity and ID, and low total concentrations are identified as a risk factor for future laminitis. An immunoturbidimetric assay has recently been validated for total adiponectin in equine samples . Leptin concentrations are useful in the prediction of laminitic episodes in ponies although they appear better associated with fat mass rather than ID in horses and therefore have the potential to mislead in EMS investigation …”

Section: Laboratory Diagnosismentioning

confidence: 99%

ECEIM consensus statement on equine metabolic syndrome

Durham¹,

Frank

McGowan

et al. 2019

Veterinary Internal Medicne

183

227

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Equine metabolic syndrome (EMS) is a widely recognized collection of risk factors for endocrinopathic laminitis. The most important of these risk factors is insulin dysregulation (ID). Clinicians and horse owners must recognize the presence of these risk factors so that they can be targeted and controlled to reduce the risk of laminitis attacks. Diagnosis of EMS is based partly on the horse's history and clinical examination findings, and partly on laboratory testing. Several choices of test exist which examine different facets of ID and other related metabolic disturbances. EMS is controlled mainly by dietary strategies and exercise programs that aim to improve insulin regulation and decrease obesity where present. In some cases, pharmacologic aids might be useful. Management of an EMS case is a long‐term strategy requiring diligence and discipline by the horse's carer and support and guidance from their veterinarians.

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“…Although serum leptin concentration correlates well with adipose tissue mass, there is little correlation with ID and laminitis risk, making its value over clinical assessment limited (Buff et al, 2002;Bamford et al, 2016a), and it is not currently offered commercially in the UK. Adiponectin production typically decreases with increasing adiposity, though this is breed and diet dependent (Bamford et al, 2016b), and low total adiponectin concentration is associated with risk of laminitis (Wray et al, 2013;Schultz et al, 2014;Menzies-Gow et al, 2018), such that adiponectin is a potentially useful marker of pathological adiposity. Of the tests that are offered in the UK, only an immunoturbidometric assay for total adiponectin has been shown to be reliable and to correlate with laminitis risk; other methods generate inconsistent results (Menzies-Gow et al, 2018).…”

Section: Laboratory Testsmentioning

confidence: 99%

Equine obesity: current perspectives

Rendle¹,

Argo

Bowen³

et al. 2018

UK-Vet Equine

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and splits his time between leading the internal medicine and critical care services and running the referral laboratory. Since graduating from The University of Bristol in 2001 he has worked in universities in both the UK and Australia but has spent most of his career in private equine practice in the UK. He is actively involved in all fields of equine medicine and has published on a range of topics, but has particular interests in endocrinology, gastroenterology and respiratory disease. He holds the RCVS certificate in equine medicine and was awarded a masters degree from The University of Glasgow.

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Validity and application of immunoturbidimetric and enzyme‐linked immunosorbent assays for the measurement of adiponectin concentration in ponies

Abstract: The IT method is suitable for measurement of equine TAC. TAC is lower in ponies with previous or future laminitis. The ELISA methods are not suitable for measurement of equine HMWAC or TAC.

Cited by 10 publications

References 19 publications

The cresty neck score is an independent predictor of insulin dysregulation in ponies

The cresty neck score is an independent predictor of insulin dysregulation in ponies

ECEIM consensus statement on equine metabolic syndrome

Equine obesity: current perspectives

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