The ielation of bacterial virulence and avirulence to crown-gall tumor induction under otheriwise suitalle conditions of host suseeptibility, temperature, etc., is one of the more interesting aspects of the "erown gall" problem. There is little doubt that elucidation of this problem wAill aidl in undcerstanding the nmechanisms Nhereby virulent bacteria affect host cells. This paper deals with experimenits designed to deteImine whethe rvirulence, as determined by ability to induce tumor formation, ean be transmitte(d to nonvirulent species in the genus Agrobacterium and other bacteriai. MATERIAL AND METHODS The organisms used in this study were: Agrobacterium tuntefaciens, stIrain S5-6, which is highly virulent for tomato and Bryophyllutni but aviIrulent for bean (Department of Botany, University of Chicago); A. tuniefaciens, strain NAv-1, which is an avirulent isolate (A. J. Riker, Department of Plant Pathology, University of Wisconsin); A. tumefaciens, strain IIBNV6, which is an avirulent isolate (A.